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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 08-05-2012, 09:37 PM   #1 (permalink)
 
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Default Phytotech is Autoclavable TC media?

I was reading somewhere that some things in TC culture media are not autoclavable. I ordered my phytotech stuff the other day and was wondering if this is true or not. I just don't want to be wasting my media.
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Old 08-06-2012, 01:55 AM   #2 (permalink)
 
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Default Re: Phytotech is Autoclavable TC media?

It's supplied as a ready made media with everything you need for TC, everything will be autoclavable, don't worry.
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Old 08-06-2012, 11:29 AM   #3 (permalink)
 
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Default Re: Phytotech is Autoclavable TC media?

One of the mediums doesn't have the gelling agent or sugar correct? I think Gabe mentioned this recently so I will have to add that though correct?
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Old 08-06-2012, 02:59 PM   #4 (permalink)
 
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Default Re: Phytotech is Autoclavable TC media?

Yes, but they are both autoclavable.
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Old 08-07-2012, 03:40 PM   #5 (permalink)
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Default Re: Phytotech is Autoclavable TC media?

Thirty some odd years ago, when I did my first TC experiments, the media was not available pre-mixed. You had to mix up about 26 chemicals yourself. After I had done the mixing, I learned that a couple of the ingredients were "Heat labile", liable to change when heated. The experiments, however, worked anyway.
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