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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 11-11-2008, 04:14 PM   #1 (permalink)
 
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Default Photoautotrophic Micropropagation

Yet another way to micropropagate plants, this is all about activating the plants own ability to generate all necesary PGRs and let it grow in sugar-hormone free media under high levels of Co2 strong illumination and a low relative humidity. no sugar is needed because the plant is stimulated by the raised illumination to photosynthezise and use the Co2 as a carbon source. by eliminating sugar you eliminate most contamination problems. the explants will be single nodes with 1 or 2 leaves attached to allow photosythesis. All this happens in a closed large vessel with hundreds of plants in regular trays using fibrous substrates such as cellulose vermiculite mixtures, the sytems stimulates root growth and plants need not aclimatize!! they come out fully hardened and rooted !! the most important factor is the C02 level, values of 1500 to 3000 ppm are discussed, the vessel needs to be force ventilated to keep these levels at a constant.

it is a very elegant way of producing plantlets, by lowering the humidity the plant will "sweat" away moisture and suck up nutrients, there is no longer a need to discover the perfect medium nor do you need to invest in PGRs or sugar, it will fix Co2 into plant matter along the way grow roots and be hardened by the forced ventilation, all you need to do is ventilate efficiently keep Co2 at the right level and pump up the PPF (light)

Bananas do great in this system pioneered by a japanese scientist named Toyoki Kozai.

I have very detailed discriptions on how to build these vessels, have sofar had no use for such a system but would wilingly suply info for people interested as long as experiences are shared. i will attach a few publications.
one on coffee and banana and one more general explaining it in more detail

regards
Dave
Attached Images
File Type: pdf photoautotrophic banana-coffee.pdf (188.6 KB, 55 views)
File Type: pdf photoautotrophic microprop.pdf (332.5 KB, 49 views)
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Old 11-11-2008, 04:34 PM   #2 (permalink)
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Default Re: Photoautotrophic Micropropagation

If I understand this right, you still need to micropropagate them with standard in vitro techniques to produce the plantlets to be grown in the photoautotrophic system. I suppose it may help if you cannot harden off the plantlets (which is very easy though) after you have cultured them. I can see this as valuable in generating rooted cuttings from some dicots, but it doesn't seem to be too valuable for bananas in my opinion, or at least as I understand it...unless I'm missing something. Thanks for sharing though!
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Old 11-12-2008, 01:11 AM   #3 (permalink)
 
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Default Re: Photoautotrophic Micropropagation

Gabe, the difference lies in the scale up, you can turn this into a closed system as large as you want it to be. we have working facilities in the Netherlands right now that cut costs by 40 % and culture times by half.
i will try to get more detailed publications. as to how good it will be for banana ... im not quite sure yet. To me not having to work elaborate protocols seems very apealing .. dont it to you?
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Old 11-12-2008, 02:29 AM   #4 (permalink)
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Default Re: Photoautotrophic Micropropagation

I can see the value in it for some other plants, but for bananas they need to be tissue cultured in some way prior to putting them into the photoautotropic system. For bananas, it seems that if you made it that far then you are at the point where you could also just put them into soil and grow them without any more lab work. Even in the paper by Kozai, it states the bananas were cultured on MS media prior to induction into the photoautotrophic system. For example, just today I transferred 36 banana explants (Senorita for those curious) from TC vessels to soil where they will continue growth into normal plants (on my dorm room window sill no less, haha), as I understand it, this is the stage at which you could also transfer to the photoautotrophic system if you wanted them to grow faster after culturing I suppose.

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Old 11-12-2008, 08:01 AM   #5 (permalink)
 
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Default Re: Photoautotrophic Micropropagation

Yu are probably right, it only seems worthwhile when you are producing huge amounts of plants, then cutting growing times in half, and the added dry weight, starts to make you serious money, nevertheless i like the theorie for its elegance! (simplicity rules)
nice windowsill (how much is that nana in the window).
ps. sometime next month i will visit the laboratory of prof. Rony Swennen from the Leuven University (belgium) he specializes in bananas and has a facility with 1500 trees all species hybids etc. you can name, he has em. looking forward to that! (ill take pictures)
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Old 11-12-2008, 06:28 PM   #6 (permalink)
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Default Re: Photoautotrophic Micropropagation

the dutch have always been very good at cloning herbaceous plant material
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Old 01-19-2009, 01:08 AM   #7 (permalink)
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Default Re: Photoautotrophic Micropropagation

Quote:
Originally Posted by rohsen View Post
Yet another way to micropropagate plants, this is all about activating the plants own ability to generate all necesary PGRs and let it grow in sugar-hormone free media under high levels of Co2 strong illumination and a low relative humidity. no sugar is needed because the plant is stimulated by the raised illumination to photosynthezise and use the Co2 as a carbon source. by eliminating sugar you eliminate most contamination problems. the explants will be single nodes with 1 or 2 leaves attached to allow photosythesis. All this happens in a closed large vessel with hundreds of plants in regular trays using fibrous substrates such as cellulose vermiculite mixtures, the sytems stimulates root growth and plants need not aclimatize!! they come out fully hardened and rooted !! the most important factor is the C02 level, values of 1500 to 3000 ppm are discussed, the vessel needs to be force ventilated to keep these levels at a constant.

it is a very elegant way of producing plantlets, by lowering the humidity the plant will "sweat" away moisture and suck up nutrients, there is no longer a need to discover the perfect medium nor do you need to invest in PGRs or sugar, it will fix Co2 into plant matter along the way grow roots and be hardened by the forced ventilation, all you need to do is ventilate efficiently keep Co2 at the right level and pump up the PPF (light)

Bananas do great in this system pioneered by a japanese scientist named Toyoki Kozai.

I have very detailed discriptions on how to build these vessels, have sofar had no use for such a system but would wilingly suply info for people interested as long as experiences are shared. i will attach a few publications.
one on coffee and banana and one more general explaining it in more detail

regards
Dave
Dave,
Will you please repost these articles, or email them to me? I can't seem to get them to download.
PM me for my email address. Thanks!
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Old 01-19-2009, 03:14 AM   #8 (permalink)
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Default Re: Photoautotrophic Micropropagation

Quote:
Originally Posted by Chironex View Post
Dave,
Will you please repost these articles, or email them to me? I can't seem to get them to download.
PM me for my email address. Thanks!
Here you go Chironex ...

Thanks for sharing rohsen, seems interesting, when I get enough banana varieties, I will surely try micropropagation and TC.
Attached Images
File Type: pdf photoautotrophic banana-coffee.pdf (188.6 KB, 20 views)
File Type: pdf photoautotrophic microprop.pdf (332.5 KB, 18 views)
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Old 01-19-2009, 03:45 AM   #9 (permalink)
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Default Re: Photoautotrophic Micropropagation

Quote:
Originally Posted by Jack Daw View Post
Here you go Chironex ...

Thanks for sharing rohsen, seems interesting, when I get enough banana varieties, I will surely try micropropagation and TC.
Thanks for trying, but apparently the files are damaged. I get an Adobe error message that they can't be downloaded.
I have tried from 2 different computers, too.
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Old 01-19-2009, 06:23 AM   #10 (permalink)
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Default Re: Photoautotrophic Micropropagation

Quote:
Originally Posted by Chironex View Post
Thanks for trying, but apparently the files are damaged. I get an Adobe error message that they can't be downloaded.
I have tried from 2 different computers, too.
Hell no, I can read them, they are completely correctly pdf formatted.

Try these:
Code:
http://www.mediafire.com/?z4fn1j2hd4j
http://www.mediafire.com/?y25wihzdndq
If you use that Adobe crap, dispose of it quickly and replace it with NitroPDF, it's the best (and about 1GB smaller).

If I could ask you, please, could you upload in the same manner ALL your materials to propagation, tissue culturing and mail me (or pm me) the link?

Would appreciate it soooo much, thx.


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Last edited by Jack Daw : 01-19-2009 at 06:24 AM. Reason: Correction
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Old 01-19-2009, 08:34 AM   #11 (permalink)
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Default Re: Photoautotrophic Micropropagation

Scot they opend and saved for me mabe you adoby is brokin theres an fix file or repair option some were on adoby try that mabey your files have becom corputed in some way or perhaps if you are uesing Vista your socurity settings have been corupted
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Old 01-19-2009, 02:40 PM   #12 (permalink)
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Default Re: Photoautotrophic Micropropagation

Quote:
Originally Posted by Chironex View Post
Thanks for trying, but apparently the files are damaged. I get an Adobe error message that they can't be downloaded.
I have tried from 2 different computers, too.
Hi Scot,
If you're still having problems, I can send them to you via email. I just now downloaded them. I can zip them before uploading. Or, maybe I can just try to do that here.

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Old 01-19-2009, 02:59 PM   #13 (permalink)
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Default Re: Photoautotrophic Micropropagation

Thank you all. I downloaded the zip file and will try that. Thanks for the alternate codes and the Nitropdf suggestion. I will try that as well.
I can send some of the TC things to you, but it will take several hours in a single sitting, or a few days in normal time. PM your email address to me and I will send some when I have a bit of free time. Right now is very busy for me. I left the lab last night at 2:15 am.
Once again, thanks to all for your help.
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