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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 06-24-2008, 11:46 PM   #1 (permalink)
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Default My home tissue culture progress

These are some photos of my first home grown tissue cultures, which have been growing for 6 days. You can see they have turned green and are starting to grow tiny leaves. When they grow large enough and start to form real leaves, pseudostems and pups (in a few more weeks), I can separate them and put them back into culture to form more plants.




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Old 06-25-2008, 12:06 AM   #2 (permalink)
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Default Re: My home tissue culture progress

Looking good Gabe! How long has it been since you started them up to this point? And what variety(ies) is (are) this?
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Old 06-25-2008, 12:14 AM   #3 (permalink)
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Default Re: My home tissue culture progress

They are 6 days old. The close up ones are of Little Prince, and on the shot of the vessels the front left 4 are Little Prince (from the same plant), the right one is Musella lasiocarpa, and the stacked ones in the back are M. ingens and E. perrieri embryos, M. laterita and an unknown Musa.
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Old 06-25-2008, 01:09 AM   #4 (permalink)
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Default Re: My home tissue culture progress

Gabe,
Thats fascinating stuff, i know very little about tissue culture, and have always assumed it would be vey scientific technical as well as expensive to set up, is there such a thing as a home TC kit? and is it expensive to set up, i am looking forward to seeing how your TC bananas come on.


Thanks Tony
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Old 06-25-2008, 03:00 AM   #5 (permalink)
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Smile Re: My home tissue culture progress

Sorry Gabe I have just seen Kevís post on the subject.
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Old 06-25-2008, 03:29 AM   #6 (permalink)
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Default Re: My home tissue culture progress

Nice one Gabe. If the Ingens take off you will be very popular with all the members on here ( not that you arn't anyway)

Good luck with them. I shall follow this thread with interest
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Old 06-25-2008, 05:43 AM   #7 (permalink)
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Default Re: My home tissue culture progress

Interesting Gabe!
Is it a lot of work to do, and could you tell me something about the hormone-works?
Do you forst add hormones to invoce rootgrowth, and next different hormones to invoke leaf and stem growth or how does that work?
Kind regards,
Remko.
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Old 06-25-2008, 06:17 AM   #8 (permalink)
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Default Re: My home tissue culture progress

Looking good, Gabe! Our resident Mad Scientist! Keep up the good work!
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Old 06-25-2008, 08:25 AM   #9 (permalink)
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Default Re: My home tissue culture progress

Hey, way to go Gabe! Where did you find that hydrometer/thermometer? Looks like it would be handy for my TC lab - once I get started.

"Dibs" on the Ingens! lol
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Last edited by Chironex : 06-25-2008 at 08:25 AM. Reason: because I forgot to claim dibs
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Old 06-25-2008, 10:33 AM   #10 (permalink)
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Default Re: My home tissue culture progress

gabe is this your first try with the home tc? i think that is so very interesting! congrats on your new babies.
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Old 06-25-2008, 10:41 AM   #11 (permalink)
 
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Default Re: My home tissue culture progress

Way to go!
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Old 06-25-2008, 11:31 AM   #12 (permalink)
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Default Re: My home tissue culture progress

Very cool! Was it difficult?
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Old 06-25-2008, 12:30 PM   #13 (permalink)
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Default Re: My home tissue culture progress

super sweet Gabe, thats awsome, i bought the book i think you were talking about, PLANTS FROM TESTTUBES, just started reading it, very interesting stuff.
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Old 06-25-2008, 04:34 PM   #14 (permalink)
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Default Re: My home tissue culture progress

thanks Gabe ones again you continue to impress me thank you for sharing that and please don't hesitate to keep informing us i cant tell you how fascinating it is to read about TC maybe some day we can send you some TC of ares!
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Old 06-25-2008, 08:30 PM   #15 (permalink)
 
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Default Re: My home tissue culture progress

Quote:
Originally Posted by Chironex View Post
Hey, way to go Gabe! Where did you find that hydrometer/thermometer? Looks like it would be handy for my TC lab - once I get started.

"Dibs" on the Ingens! lol
You can find them in pet shops in the reptile section.
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Old 06-25-2008, 08:31 PM   #16 (permalink)
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Default Re: My home tissue culture progress

I am almost finished with the book and have all of the chemicals ordered. We just need to make a laminar flow hood. Gabe sent me the instructions on how he made his, so we will be ready to go once I get there.
If you can round up couple of quart sized jars, wide-mouthed, (like Mason canning jars or a mayonnaise jar). Also, need some 4 or 6 oz baby food jars we should have just about everything we need. I have about 30 of them, but we will need a bunch eventually.


I am so excited to begin, I can't stand it!
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Old 06-25-2008, 08:42 PM   #17 (permalink)
 
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Default Re: My home tissue culture progress

that looks very intriguing! I'm stupid when it comes to tissue culturing. I've no clue what so ever of how to. can anyone explain please?
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Old 06-26-2008, 06:20 AM   #18 (permalink)
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Default Re: My home tissue culture progress

Berserker,

Use the search feature and you'll find several threads on this subject. Gabe has a really good diagram illustrating how you start with one plant and end up with many. It's in another post. You can also search his photo gallery, which contains that diagram as well.
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Old 06-26-2008, 02:29 PM   #19 (permalink)
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Default Re: My home tissue culture progress

excel there Gabe, keep 'em going. you got the Ingens to work then . I'd better think of some more exotic varieties for you to play with, 'Xishuangbannensis' maybe? or Musa Sp. Yangtse (whatever it is?).
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Old 06-26-2008, 08:06 PM   #20 (permalink)
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Default Re: My home tissue culture progress

Quote:
Originally Posted by momoese View Post
Very cool! Was it difficult?
Not difficult if you know what you are doing, but without experience may take awhile to get right.
Quote:
Originally Posted by mskitty38583 View Post
gabe is this your first try with the home tc? babies.
This is my first time doing it at home, but its my job in Hawaii, so I just took what I learned from there and modified it all to work without a lab and expensive equipment.
Quote:
Originally Posted by griphuz View Post
Interesting Gabe!
Is it a lot of work to do, and could you tell me something about the hormone-works?
Do you forst add hormones to invoce rootgrowth, and next different hormones to invoke leaf and stem growth or how does that work?
Kind regards,
Remko.
These cultures have in the media the hormone BA (benzyladenine), this suppresses root growth and shoot elongation but encourages shoot proliferation, so you can make multiple meristems on a single tissue mass. Eventually, I will use a very similar media except the BA will be absent, this will allow roots to grow, shoots to elongate, and no longer encourage multiplication, this will allow the growth of single rooted plants ready for transfer to soil.
Quote:
Originally Posted by 51st state View Post
excel there Gabe, keep 'em going. you got the Ingens to work then . I'd better think of some more exotic varieties for you to play with, 'Xishuangbannensis' maybe? or Musa Sp. Yangtse (whatever it is?).
regards
Kev
I don't know if they ingens will work or not, I just put them in culture and am in California for a week, so when I get back home next week I will be able to tell if it worked or not.
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