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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside. |
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07-09-2008, 12:16 AM | #41 (permalink) |
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Re: My home tissue culture progress
The neighbors have been saying that for years, haha. My parents are really very supportive with everything. When I first started growing plants, they would kinda oversee everything and limit the amount of plants I kept, but as time went on they realized I could handle it all better then they, and just kinda let me do whatever it is I was doing and left me alone with it. Now with the TC stuff, they are just amazed and confused enough with the whole thing they don't even care to say anything when I'm lighting torches and wielding knives in the back of the house, they just figure I must know what I'm doing (which I do), and that's good enough for them.
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07-09-2008, 12:22 AM | #42 (permalink) |
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Re: My home tissue culture progress
I just recently found this video, it has to do with what I was talking about earlier (in another thread perhaps) about the real advantages of TC and why its an important science. This is where bananas really matter, and what drives me to learn and study them more and more.
[yt]25OIKGzZZIY[/yt]
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07-09-2008, 06:21 AM | #43 (permalink) |
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Re: My home tissue culture progress
Gabe,
Just wanted to say thanks to you for taking the time to share all of this with us. I don't plan on trying it at home myself, but being a horticulturist, I've always been interested in the whole TC process. Great job! |
07-09-2008, 07:44 AM | #44 (permalink) |
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Re: My home tissue culture progress
Gabe,
thank you so much for all the fine information regarding TC. It almost makes me want to try. if i only had the space and time maybe after i get the green house built all start a lab its allot less water intensive then growing out pups at least in the beginning. have you giving any thought to are conversation regarding the contamination sources? perhaps there are people that could be contacted regarding this subject whom mite have come across this in there own Tc labs, or whom mite have discussed this with other Technions? on a slightly different note. I was thinking about how one could Tc Ae Ae. As has been discussed in other threads that it cant be done do to the fact that the variegation is not constant throw the entire meristem. I was thinking about that. As long as you got some of the two different tissues in the sample wouldn't it come true? i would think this would involve knowing exactly were the line of deviation is. and always making sure that when you divide that you get both types of tissues in the Varese samples. dos that make any sense? so in assents one would have to develop a technique for showing the two different tissues in the sample. has any one tried this? Perhaps a dye of some sort that sticks to the two in different intestacy delineating the different types of cells. Or using a microscope to see the varying lines of demarcation when cutting the sample up. Just some thoughts im sure this has been tryed but mabe not you never know!
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07-09-2008, 10:02 AM | #45 (permalink) | |
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Re: My home tissue culture progress
Quote:
I have pictures of TC Aeae and have talked in person with the lady who did them. She claimed she didn't do anything special and treated them like all the other Musa. My boss thinks she knows how to do it to keep the variegation, and we would love to experiment with it, but don't have a source for any plants on the island right now. Its actually easier to get them on the mainland than in Hawaii, and banana pups cannot be taken between islands, so they would need to be growing on Oahu already. Perhaps if I can get my hands on a few one day we will try.
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07-09-2008, 10:10 AM | #46 (permalink) | |
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Re: My home tissue culture progress
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07-09-2008, 10:19 AM | #47 (permalink) |
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Re: My home tissue culture progress
Another great video, Gabe! I find it very interesting. I wish you well w/ your studies. The banana world can use more experts.
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07-09-2008, 11:52 PM | #48 (permalink) | |
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07-10-2008, 11:41 AM | #49 (permalink) |
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Re: My home tissue culture progress
Thanks for all the info Gabe. I'm wondering- can you take a diseased plant and tissue culture it, and end up with disease free plantlets?
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07-10-2008, 12:38 PM | #50 (permalink) | |
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Re: My home tissue culture progress
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07-10-2008, 03:47 PM | #51 (permalink) |
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Re: My home tissue culture progress
WOW that video is awesome ;D
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07-10-2008, 07:11 PM | #52 (permalink) |
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Re: My home tissue culture progress
I would imagine it depends what disease it is, especially what kind (virus, fungus, insect etc...). I have read somewhere that bunchy top (a virus) does not transfer in culture, it may just be with some varieties under special circumstances though, Im not sure. I also know that other viruses definitely carry over, but I dont know any specifics.
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07-11-2008, 12:16 AM | #53 (permalink) |
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01-21-2009, 10:55 AM | #54 (permalink) |
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Re: My home tissue culture progress
plant patent #15,255, Little prince musa...you should get rid of those or pay the royalties. Watch what you culture unless you get appropriate licenses. Sorry to rain on your parade...
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01-21-2009, 11:46 AM | #55 (permalink) |
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Re: My home tissue culture progress
I believe he already killed them anyways....
On a related matter: Do we also need to pay royalties if we plant pups from a purchased plant? |
01-21-2009, 12:46 PM | #56 (permalink) |
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Re: My home tissue culture progress
As I understood it, its only patent infringement if you plan on selling patented plants. I don't know how they would catch you propagating your own plants for yourself. If they are really that concerned about it then they can come after me, but its not going to stop me from creating mats of miniature bananas to plant around the dorms! I don't plan on selling any 'Little Prince' plants, they are easy to find elsewhere and I have things in TC that I think people will be much more interested in that are under no restrictions.
As Harvey mentioned, all of my TC experiments from last summer died anyway (issues with shipping...I gave my mom directions but sometimes you just gotta do things yourself! haha). However, after a successful collecting mission at the Denver Botanic Gardens over winter break I got a happy number of them back and they are back in TC. If anyone has things they would like for me to try, I'm better prepared to do that now than I was over the summer since I'm back at my lab again.
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01-21-2009, 01:05 PM | #57 (permalink) |
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Re: My home tissue culture progress
Over the summer I had prepared tissue cultures of the following and was able to get back the ones listed as "recovered".
Some cool little red thing from China- recovered Little Prince- recovered "Red Raja" (my own temporary name for an unknown)- recovered M. balbisiana x M. dasycarpa #1- recovered Musella lasiocarpa- recovered Siam Ruby- lost, but can get it here now so not a problem Goldfinger- lost, but can get it here Truly Tiny- lost M. cheesmanii- lost California Gold- lost Ensete perrieri- lost Ensete ventricosum "Mozambique form"- lost, might be able to get back someday Hua Moa Freakazoid sport- lost...probably forever probably interesting unknown seedling- lost...never to return
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01-21-2009, 01:27 PM | #58 (permalink) |
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Re: My home tissue culture progress
Why is it that when we buy stone fruit trees, roses, etc. they all have a plant patent label on them. However, I've seen Little Prince without such a tag?
Sandy, when you buy a flat of TC plants of something like Little Prince, do they each come with a tag for you to include when you sell them? Also, I'm not familiar with the patenting of something that self propagates such as bananas. How does the patent laws apply to such things? |
01-21-2009, 04:42 PM | #59 (permalink) |
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Re: My home tissue culture progress
When you buy a patented plant to resell, you pay a royalty on the plant that goes back to the developer of the plant. That's why people want to patent them...
They should come with a tag that states it's a patented plant. If you want to sell those plants, you have to pay the royalty. You're not authorized to reproduce the plant and sell it. I guess for yourself it would be ok, I'm not really clear on that. I just know I can't do it and then sell them or pass them on as named varieties. Big no no! Since Gabe wants to go further into this, possibly for $$, I thought he might want to know to confine himself to non-patented varieties. I have a friend who purchased propagation licenses on several plants--it was expensive. Then he has to collect the royalties on the plants he produces and sells and forward it back to the owner. Crazy huh? And he's supposed to keep tabs on how many he produces vs how many sells in case they want to audit him.
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01-21-2009, 10:36 PM | #60 (permalink) |
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Re: My home tissue culture progress
Thank you for the info, but as stated, I am not selling any of the patented varieties, or for that matter, any varieties at this point. Really all I want is to do is have a Little Prince desktop banana in my dorm, and to try a few outside in my garden. I put as much as I can into TC backup because it is very likely the plants outside will contract Bunchy Top Virus at some point. With the backups from the lab I can generate more, but if I only have one small plant and I lose it, then I will no longer have that variety.
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