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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 02-04-2009, 03:33 PM   #61 (permalink)
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Default Re: My home tissue culture progress

I hate to seem like I'm not letting this go, but it's not just if you're going to sell them. It's if you're propagating them at ALL. It's just a no-no, no matter how tempting it is. Even if you have "warm fuzzy feelings for the plant and just don't want to lose it". If you lose it, you buy another. Sorry, I didn't make up the rules, I just play within them.
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Rights Conveyed by a Plant Patent

Grant of a patent for a plant precludes others from asexually reproducing or selling or using the patented plant. A plant patent is regarded as limited to one plant, or genome. A sport or mutant of a patented plant would not be considered to be of the same genotype, would not be covered by the plant patent to the parent plant, and would, itself, be separately patentable, subject to meeting the requirements of patentability. A plant patent expires 20 years from the filing date of the patent application. As with utility applications, when the plant patent expires, the subject matter of the patent becomes public domain.
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Old 02-04-2009, 05:23 PM   #62 (permalink)
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Default Re: My home tissue culture progress

Sandy, I wish you would drop this or start a new thread and this is taking on the character of "flaming". First you assumed Gabe was "going further with this" and now you're criticizing something which in just your last post you had guessed "would be okay".

Quote:
Originally Posted by sandy0225 View Post
When you buy a patented plant to resell, you pay a royalty on the plant that goes back to the developer of the plant. That's why people want to patent them...
They should come with a tag that states it's a patented plant. If you want to sell those plants, you have to pay the royalty. You're not authorized to reproduce the plant and sell it. I guess for yourself it would be ok, I'm not really clear on that.
I just know I can't do it and then sell them or pass them on as named varieties. Big no no! Since Gabe wants to go further into this, possibly for $$, I thought he might want to know to confine himself to non-patented varieties.
I have a friend who purchased propagation licenses on several plants--it was expensive. Then he has to collect the royalties on the plants he produces and sells and forward it back to the owner. Crazy huh? And he's supposed to keep tabs on how many he produces vs how many sells in case they want to audit him.
Now, I'm sure you are aware, that some of your own online customers should be paying "use" taxes for their online purchases from you (though plants grown for food are not subject to California residents they are in some other states). Do you take the same steps to enforce these laws and reminding your customers about their obligations?

Sorry...end of rant.

Moving on,

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Old 02-04-2009, 05:49 PM   #63 (permalink)
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Default Re: My home tissue culture progress

Sorry, I wasn't trying to flame anybody. I just thought this stuff was interesting. I didn't know the answers so I researched it more, and updated with what I found out. I thought people might want to know. But I guess we really don't...
Sorry to offend anyone....
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Old 04-19-2009, 03:52 AM   #64 (permalink)
 
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Hiya Re: My home tissue culture progress

Quote:
Originally Posted by Gabe15 View Post
Here is a short video of the actual tissue culture initiation. It starts out with a plant that has been whittled down to a piece of tissue with some pseuodstem above the meristem and some corm below the meristem. The two clips are of two different plants, but nothing is missed in the process. This is part of a video I'm working on detailing the full process.

[yt]p4DUwSGJYNE[/yt]

hi,
if you have a full video or procedure for banana plant tissue culture
then please send me on jigar1231@yahoo.com
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Old 04-20-2009, 08:32 AM   #65 (permalink)
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Default Re: My home tissue culture progress

Considering that you are experts with quite many experience, I would like to ask you some details.
I have been wondering for quite some time:

- What is the precise list of tools, liquids and materials needed for succesful tissue culturing process?
- How much would it cost to make a smaller lab for not large amounts of plants?
- Did you use someone's advice/help on fitting your lab with tools...?

As I'm growing more and more interested about this topic, I would be really glad, if you could sum up your experience and knowledge into a list of all the necessary things, materials and stuff and their apx. costs
What are the special requirements for the room (future labs), meaning like sun/no sun, wet/dry, cold/medium/hot and so on... just everything that crosses your mind. That estimate would be lovely, just to see how much it would be.

If there's been a post like this already, I apologize, but I didn't find it. Just link me. Thanks
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Old 04-20-2009, 09:06 AM   #66 (permalink)
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Default Re: My home tissue culture progress

That the spirit Jack. Do some TC for Slavic members
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Old 04-20-2009, 09:12 AM   #67 (permalink)
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Default Re: My home tissue culture progress

Quote:
Originally Posted by Dalmatiansoap View Post
That the spirit Jack. Do some TC for Slavic members
That wasn't the point, I'm just curious in knowing, how much would it cost. I'm not about to start a lab, at least not until I fully understand what goes with it, what are the monthly/annual costs, what can I expect of building problems etc.

I also take in consideration going to our university of Medicine or Natural Sciences and ask them, if it would be possible to use the laboratory, considering some "rent" and of course, bringing my own material. It all depends on how much the total costs are and how much material and technology is needed. And the point was "European", not just "Slavic" ... There are lots of eager and pashionate collectors in the EU, the User Map shows that as well.
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Old 04-20-2009, 09:18 AM   #68 (permalink)
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Default Re: My home tissue culture progress

I was just joking Jack and have to admit that I would also like that we in Europe have a few naner TC experts.
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Old 04-20-2009, 09:44 AM   #69 (permalink)
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Default Re: My home tissue culture progress

Jack, all of this information can be found at: Home Tissue Culture* Group
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Old 04-20-2009, 09:50 AM   #70 (permalink)
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Default Re: My home tissue culture progress

Anyone? I know guys you TC a lot... Chironex? Gabe?
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Old 04-20-2009, 09:54 AM   #71 (permalink)
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Default Re: My home tissue culture progress

The lab can be set-up for under $50. It is not expensive for a home user to do. Once you purchase the chemicals and culture vessels, then all you need are somewhere to keep them and some lights.
As they start to grow and be sub-cultured, that's when the costs increase. You have to buy more media, more containers and it takes more space, so you need more lights. It is best to keep it small production to prevent the cost escalation.
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Old 04-20-2009, 10:47 AM   #72 (permalink)
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Quote:
Originally Posted by Chironex View Post
The lab can be set-up for under $50. It is not expensive for a home user to do. Once you purchase the chemicals and culture vessels, then all you need are somewhere to keep them and some lights.
As they start to grow and be sub-cultured, that's when the costs increase. You have to buy more media, more containers and it takes more space, so you need more lights. It is best to keep it small production to prevent the cost escalation.
Thank you very much Scot, I'll do some more studying. If I have questions, is it OK just to pm you?
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Old 04-20-2009, 08:15 PM   #73 (permalink)
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Default Re: My home tissue culture progress

You can PM me, but you may have to wait for a reply. I have been getting lots of PM's and I have become very busy at work, so I don't always get to the PM's right away.
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Old 12-18-2009, 09:07 AM   #74 (permalink)
 
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Default Re: My home tissue culture progress

hi Gabe,
Do u mind to tell ime wats de media dat u used to culture banana at home?
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Old 02-16-2010, 05:37 PM   #75 (permalink)
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Default Re: My home tissue culture progress

Hi Gabe
Glad to hear you work in Tc also like me. can you post your tc plant? and what type you tc now?
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Old 08-03-2012, 12:14 AM   #76 (permalink)
 
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Default Re: My home tissue culture progress

Quote:
Originally Posted by Gabe15 View Post
Over the summer I had prepared tissue cultures of the following and was able to get back the ones listed as "recovered".

Some cool little red thing from China- recovered
Little Prince- recovered
"Red Raja" (my own temporary name for an unknown)- recovered
M. balbisiana x M. dasycarpa #1- recovered
Musella lasiocarpa- recovered

Siam Ruby- lost, but can get it here now so not a problem
Goldfinger- lost, but can get it here

Truly Tiny- lost
M. cheesmanii- lost
California Gold- lost
Ensete perrieri- lost
Ensete ventricosum "Mozambique form"- lost, might be able to get back someday

Hua Moa Freakazoid sport- lost...probably forever
probably interesting unknown seedling- lost...never to return

Did you ever reculture a california gold?
and why do you think you lost this one?
Thanks
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Old 08-03-2012, 12:44 AM   #77 (permalink)
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Default Re: My home tissue culture progress

That was quite awhile ago, I don't remember exactly, but I think I lost the cultures because I was transporting them in the TC bags and some of them broke. I never tried that one again.
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