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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 07-09-2008, 12:16 AM   #41 (permalink)
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Default Re: My home tissue culture progress

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Originally Posted by harveyc View Post
My 10 year old son enjoyed the video too. But it makes me wonder what your parents think of this. Are the neighbors talking about the strange kid down the block growing bananas in test tubes????
The neighbors have been saying that for years, haha. My parents are really very supportive with everything. When I first started growing plants, they would kinda oversee everything and limit the amount of plants I kept, but as time went on they realized I could handle it all better then they, and just kinda let me do whatever it is I was doing and left me alone with it. Now with the TC stuff, they are just amazed and confused enough with the whole thing they don't even care to say anything when I'm lighting torches and wielding knives in the back of the house, they just figure I must know what I'm doing (which I do), and that's good enough for them.
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Old 07-09-2008, 12:22 AM   #42 (permalink)
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Default Re: My home tissue culture progress

I just recently found this video, it has to do with what I was talking about earlier (in another thread perhaps) about the real advantages of TC and why its an important science. This is where bananas really matter, and what drives me to learn and study them more and more.

[yt]25OIKGzZZIY[/yt]
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Old 07-09-2008, 06:21 AM   #43 (permalink)
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Default Re: My home tissue culture progress

Gabe,

Just wanted to say thanks to you for taking the time to share all of this with us. I don't plan on trying it at home myself, but being a horticulturist, I've always been interested in the whole TC process. Great job!
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Old 07-09-2008, 07:44 AM   #44 (permalink)
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Default Re: My home tissue culture progress

Gabe,
thank you so much for all the fine information regarding TC. It almost makes me want to try. if i only had the space and time maybe after i get the green house built all start a lab its allot less water intensive then growing out pups at least in the beginning. have you giving any thought to are conversation regarding the contamination sources? perhaps there are people that could be contacted regarding this subject whom mite have come across this in there own Tc labs, or whom mite have discussed this with other Technions? on a slightly different note. I was thinking about how one could Tc Ae Ae. As has been discussed in other threads that it cant be done do to the fact that the variegation is not constant throw the entire meristem. I was thinking about that. As long as you got some of the two different tissues in the sample wouldn't it come true? i would think this would involve knowing exactly were the line of deviation is. and always making sure that when you divide that you get both types of tissues in the Varese samples. dos that make any sense? so in assents one would have to develop a technique for showing the two different tissues in the sample. has any one tried this? Perhaps a dye of some sort that sticks to the two in different intestacy delineating the different types of cells. Or using a microscope to see the varying lines of demarcation when cutting the sample up. Just some thoughts im sure this has been tryed but mabe not you never know!
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Old 07-09-2008, 10:02 AM   #45 (permalink)
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Default Re: My home tissue culture progress

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have you giving any thought to are conversation regarding the contamination sources? perhaps there are people that could be contacted regarding this subject whom mite have come across this in there own Tc labs, or whom mite have discussed this with other Technions?
I emailed someone who has tried it before, and also failed, but am waiting to hear back on their experiences. I also know a few other experts in the field, and will see what they think about it.

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I was thinking about how one could Tc Ae Ae.
I have pictures of TC Aeae and have talked in person with the lady who did them. She claimed she didn't do anything special and treated them like all the other Musa. My boss thinks she knows how to do it to keep the variegation, and we would love to experiment with it, but don't have a source for any plants on the island right now. Its actually easier to get them on the mainland than in Hawaii, and banana pups cannot be taken between islands, so they would need to be growing on Oahu already. Perhaps if I can get my hands on a few one day we will try.
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Old 07-09-2008, 10:10 AM   #46 (permalink)
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I emailed someone who has tried it before, and also failed, but am waiting to hear back on their experiences. I also know a few other experts in the field, and will see what they think about it.


I have pictures of TC Aeae and have talked in person with the lady who did them. She claimed she didn't do anything special and treated them like all the other Musa. My boss thinks she knows how to do it to keep the variegation, and we would love to experiment with it, but don't have a source for any plants on the island right now. Its actually easier to get them on the mainland than in Hawaii, and banana pups cannot be taken between islands, so they would need to be growing on Oahu already. Perhaps if I can get my hands on a few one day we will try.
thanks for the speedy reply well the Ae Ae would by far be one of the most profitable Tc projects do to the high cost of the naturally grown plants id like to see thees rare plants proliferate so the price could moderate letting more people have the joy of growing these vary beautiful musa
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Old 07-09-2008, 10:19 AM   #47 (permalink)
 
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Default Re: My home tissue culture progress

Another great video, Gabe! I find it very interesting. I wish you well w/ your studies. The banana world can use more experts.
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Old 07-09-2008, 11:52 PM   #48 (permalink)
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Default Re: My home tissue culture progress

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Originally Posted by Gabe15 View Post
I have pictures of TC Aeae and have talked in person with the lady who did them. She claimed she didn't do anything special and treated them like all the other Musa. My boss thinks she knows how to do it to keep the variegation, and we would love to experiment with it, but don't have a source for any plants on the island right now. Its actually easier to get them on the mainland than in Hawaii, and banana pups cannot be taken between islands, so they would need to be growing on Oahu already. Perhaps if I can get my hands on a few one day we will try.
Maybe you can get together with someone here in the domestic states and teach them your boss's hypothetical method! I'll volunteer!!!! Too bad I don't have an AeAe YET!
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Old 07-10-2008, 11:41 AM   #49 (permalink)
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Default Re: My home tissue culture progress

Thanks for all the info Gabe. I'm wondering- can you take a diseased plant and tissue culture it, and end up with disease free plantlets?
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Old 07-10-2008, 12:38 PM   #50 (permalink)
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Default Re: My home tissue culture progress

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Originally Posted by damaclese View Post
thanks for the speedy reply well the Ae Ae would by far be one of the most profitable Tc projects do to the high cost of the naturally grown plants id like to see thees rare plants proliferate so the price could moderate letting more people have the joy of growing these vary beautiful musa
Wow,that would be great! Look what it did for Siam Ruby. I see them as low as $9.95 on Ebay now.
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Old 07-10-2008, 03:47 PM   #51 (permalink)
 
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WOW that video is awesome ;D
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Old 07-10-2008, 07:11 PM   #52 (permalink)
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Thanks for all the info Gabe. I'm wondering- can you take a diseased plant and tissue culture it, and end up with disease free plantlets?
I would imagine it depends what disease it is, especially what kind (virus, fungus, insect etc...). I have read somewhere that bunchy top (a virus) does not transfer in culture, it may just be with some varieties under special circumstances though, Im not sure. I also know that other viruses definitely carry over, but I dont know any specifics.
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Old 07-11-2008, 12:16 AM   #53 (permalink)
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There is no excuse for still having grass. I haven't mowed in 20 years. With all that space, I could plant another 100 bananas.
My new hero...

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Old 01-21-2009, 10:55 AM   #54 (permalink)
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Default Re: My home tissue culture progress

plant patent #15,255, Little prince musa...you should get rid of those or pay the royalties. Watch what you culture unless you get appropriate licenses. Sorry to rain on your parade...
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Old 01-21-2009, 11:46 AM   #55 (permalink)
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I believe he already killed them anyways....

On a related matter: Do we also need to pay royalties if we plant pups from a purchased plant?
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Old 01-21-2009, 12:46 PM   #56 (permalink)
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As I understood it, its only patent infringement if you plan on selling patented plants. I don't know how they would catch you propagating your own plants for yourself. If they are really that concerned about it then they can come after me, but its not going to stop me from creating mats of miniature bananas to plant around the dorms! I don't plan on selling any 'Little Prince' plants, they are easy to find elsewhere and I have things in TC that I think people will be much more interested in that are under no restrictions.

As Harvey mentioned, all of my TC experiments from last summer died anyway (issues with shipping...I gave my mom directions but sometimes you just gotta do things yourself! haha). However, after a successful collecting mission at the Denver Botanic Gardens over winter break I got a happy number of them back and they are back in TC. If anyone has things they would like for me to try, I'm better prepared to do that now than I was over the summer since I'm back at my lab again.
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Old 01-21-2009, 01:05 PM   #57 (permalink)
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Over the summer I had prepared tissue cultures of the following and was able to get back the ones listed as "recovered".

Some cool little red thing from China- recovered
Little Prince- recovered
"Red Raja" (my own temporary name for an unknown)- recovered
M. balbisiana x M. dasycarpa #1- recovered
Musella lasiocarpa- recovered

Siam Ruby- lost, but can get it here now so not a problem
Goldfinger- lost, but can get it here

Truly Tiny- lost
M. cheesmanii- lost
California Gold- lost
Ensete perrieri- lost
Ensete ventricosum "Mozambique form"- lost, might be able to get back someday

Hua Moa Freakazoid sport- lost...probably forever
probably interesting unknown seedling- lost...never to return
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Old 01-21-2009, 01:27 PM   #58 (permalink)
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Why is it that when we buy stone fruit trees, roses, etc. they all have a plant patent label on them. However, I've seen Little Prince without such a tag?

Sandy, when you buy a flat of TC plants of something like Little Prince, do they each come with a tag for you to include when you sell them? Also, I'm not familiar with the patenting of something that self propagates such as bananas. How does the patent laws apply to such things?
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Old 01-21-2009, 04:42 PM   #59 (permalink)
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When you buy a patented plant to resell, you pay a royalty on the plant that goes back to the developer of the plant. That's why people want to patent them...
They should come with a tag that states it's a patented plant. If you want to sell those plants, you have to pay the royalty. You're not authorized to reproduce the plant and sell it. I guess for yourself it would be ok, I'm not really clear on that.
I just know I can't do it and then sell them or pass them on as named varieties. Big no no! Since Gabe wants to go further into this, possibly for $$, I thought he might want to know to confine himself to non-patented varieties.
I have a friend who purchased propagation licenses on several plants--it was expensive. Then he has to collect the royalties on the plants he produces and sells and forward it back to the owner. Crazy huh? And he's supposed to keep tabs on how many he produces vs how many sells in case they want to audit him.
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Old 01-21-2009, 10:36 PM   #60 (permalink)
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Since Gabe wants to go further into this, possibly for $$, I thought he might want to know to confine himself to non-patented varieties.
Thank you for the info, but as stated, I am not selling any of the patented varieties, or for that matter, any varieties at this point. Really all I want is to do is have a Little Prince desktop banana in my dorm, and to try a few outside in my garden. I put as much as I can into TC backup because it is very likely the plants outside will contract Bunchy Top Virus at some point. With the backups from the lab I can generate more, but if I only have one small plant and I lose it, then I will no longer have that variety.
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