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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 06-30-2008, 06:36 AM   #21 (permalink)
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Default Re: My home tissue culture progress

Gabe, please put me on the list for Ingens. Still trying to find some seeds or plants, but no luck so far.
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Old 06-30-2008, 11:34 AM   #22 (permalink)
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Red face Re: My home tissue culture progress

Gabe,

They are looking great. I have a question I received some TC of Siam Ruby this winter, one of which stayed green. Does this mean any pups produced by the TC will also be green or is there a chance of the pups being red?
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Old 06-30-2008, 02:10 PM   #23 (permalink)
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Default Re: My home tissue culture progress

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Originally Posted by Chironex View Post
Gabe, please put me on the list for Ingens. Still trying to find some seeds or plants, but no luck so far.
Hiya
How many do you want (seeds that is) I supplied gabe with his a couple months back. $2 per seed though I'm afraid, includes shippping though
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Old 06-30-2008, 02:14 PM   #24 (permalink)
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Default Re: My home tissue culture progress

Good for you Gabe, you are in the forefront of the hobby.
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Old 06-30-2008, 03:17 PM   #25 (permalink)
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Default Re: My home tissue culture progress

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Originally Posted by tophersmith View Post
Gabe,

They are looking great. I have a question I received some TC of Siam Ruby this winter, one of which stayed green. Does this mean any pups produced by the TC will also be green or is there a chance of the pups being red?
They will likely all stay green.
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Old 07-08-2008, 04:27 PM   #26 (permalink)
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Default Re: My home tissue culture progress

UPDATE:
Things are going fairly well. The M. ingens seeds did not work (as expected) so I'm still working on that one. Pretty much everything else worked and is growing nicely. I had one unexpected contamination in my Musella vessel, but I may have corrected it (I'll know in a few days).

This 'Little Prince' culture may look like the creature from the black lagoon, but it is actually growing perfectly and is ready to be split into more plants.


This Musella lasiocarpa is growing great, but unfortunately there is some bacterial contamination (the off-white goo creeping around the edges). I made up a way to save these kinds of contaminations, but it doesn't work all the time so we shall see if it can be saved.
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Old 07-08-2008, 04:35 PM   #27 (permalink)
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Default Re: My home tissue culture progress

Looks good Gabe! Gabe, I have a request for you, next time you go cutting into a plant to get the meristem would you mind taking pictures to share?!?!? Thanks if you can!
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Old 07-08-2008, 06:10 PM   #28 (permalink)
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Default Re: My home tissue culture progress

Here is a short video of the actual tissue culture initiation. It starts out with a plant that has been whittled down to a piece of tissue with some pseuodstem above the meristem and some corm below the meristem. The two clips are of two different plants, but nothing is missed in the process. This is part of a video I'm working on detailing the full process.

[yt]p4DUwSGJYNE[/yt]
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Old 07-08-2008, 06:38 PM   #29 (permalink)
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Default Re: My home tissue culture progress

Great work, Gabe. I'm constantly amazed by your banana skills.
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Old 07-08-2008, 06:56 PM   #30 (permalink)
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Default Re: My home tissue culture progress

That's awsome Gabe... Now I'm curious though... the last part of the video you had taken the meristem and put it into a type of mason jar, but then you took out something else that you seemed to have been culturing, and put two of the other pieces that you had removed into that one... And what happened with the one you removed? LOL
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Old 07-08-2008, 07:15 PM   #31 (permalink)
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Default Re: My home tissue culture progress

Quote:
Originally Posted by jpfloors View Post
That's awsome Gabe... Now I'm curious though... the last part of the video you had taken the meristem and put it into a type of mason jar, but then you took out something else that you seemed to have been culturing, and put two of the other pieces that you had removed into that one... And what happened with the one you removed? LOL
In that vessel was an explant of another variety that was growing fine for a few weeks and had no problems, however, instead of using a new vessel, I just took out that explant and put the new ones in since the media was still good and it had no visible contamination. In our lab, if a vessel is contaminated and the plants are large enough, we plant them directly into soil without the normal transfer to the rooting medium to grow roots and leaves while in culture. This doesn't always work, and this explant is on the very smallest end of explants to be used like this, but I figured it was worth a shot to see if it will root in soil.

Here is that explant.

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Old 07-08-2008, 08:05 PM   #32 (permalink)
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Default Re: My home tissue culture progress

Quote:
Originally Posted by Gabe15 View Post
This Musella lasiocarpa is growing great, but unfortunately there is some bacterial contamination (the off-white goo creeping around the edges). I made up a way to save these kinds of contaminations, but it doesn't work all the time so we shall see if it can be saved.
Gabe, Curious to know what the contamination is and also the way you are trying to save it.
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Old 07-08-2008, 08:20 PM   #33 (permalink)
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Default Re: My home tissue culture progress

I noticed that the vessels were not ventilated. Do you find that there is less contamination risk this way, or do you transfer to ventilated vessels later on?
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Old 07-08-2008, 08:27 PM   #34 (permalink)
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Default Re: My home tissue culture progress

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Gabe, Curious to know what the contamination is and also the way you are trying to save it.
Its a bacteria. A few times before, I've dipped the explant into the bleach solution, then into the alcohol and burned off all the alcohol and transfered to a new, clean vessel. If done "lightly" enough, I'm sure it damages the outer layers but the inner tissue (where the meristem is) seems to stay protected. I haven't done this very many times, so figured some more experimentation was in order.

Quote:
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I noticed that the vessels were not ventilated. Do you find that there is less contamination risk this way, or do you transfer to ventilated vessels later on?
The box ones allow for gas exchange, normally we seal off the opening with 3M micropore surgical tape (which allows for filtered gas exchange, very useful), however since I forgot to add it to my order, I instead just try to close the lid tightly and then put it in a plastic baggie. The mason jars are probably pretty well sealed (though I do break the tight seal formed by pressure cooking), however, since I'm only leaving these in culture short term, I'm not too worried about it. But then again, its all part of the home TC experimenting, so I'll find out soon enough.
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Old 07-08-2008, 08:38 PM   #35 (permalink)
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Default Re: My home tissue culture progress

Wow, kinda like roasting marshmallows, eh? What dilution of bleach solution, 10% or 20%? I also read where you can use some round band-aids that have a antiseptic gauze to cover a nail hole in the mason jar lids. I think Carol Stiff suggests these as a cheaper alternative than the 3M micropore tape. She says that they will remain in place after autoclaving a few times, too.
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Old 07-08-2008, 08:41 PM   #36 (permalink)
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Quote:
Originally Posted by Chironex View Post
Wow, kinda like roasting marshmallows, eh? What dilution of bleach solution, 10% or 20%? I also read where you can use some round band-aids that have a antiseptic gauze to cover a nail hole in the mason jar lids. I think Carol Stiff suggests these as a cheaper alternative than the 3M micropore tape. She says that they will remain in place after autoclaving a few times, too.
Hmmm...interesting on the bandaid bit, I'll add it to my things to test. Thanks for the tip!

I use a ~25% bleach solution, but I just sorta eyed it out to 1/4, no exact measuring.
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Old 07-08-2008, 10:39 PM   #37 (permalink)
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Default Re: My home tissue culture progress

gabe i have to say this is interesting stuff. but i also do have to say it looks like something my kitty ate. lol. i really like this genetic stuff.
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Old 07-08-2008, 10:50 PM   #38 (permalink)
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Default Re: My home tissue culture progress

And to think of the money I have spent on graduated cylinders, pipettes and such....oh well. Just my addiction again.
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Old 07-08-2008, 10:53 PM   #39 (permalink)
 
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Default Re: My home tissue culture progress

Great video, intresting to see banana plants being made.
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Old 07-09-2008, 12:02 AM   #40 (permalink)
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Default Re: My home tissue culture progress

My 10 year old son enjoyed the video too. But it makes me wonder what your parents think of this. Are the neighbors talking about the strange kid down the block growing bananas in test tubes????
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