My home TC lab is open, Finally!

Chironex · 10-04-2008, 08:40 PM

Just finished setting up a few things and starting my first culture today, an embryo rescue is first on my agenda.

The flow hood is in my photo gallery along with some pics of the recently received starts.

Will continue to post progress reports as more gets going.

xavierdlc61887 · 10-04-2008, 08:43 PM

nice man hey did u ever send the seeds cause i never got anything...if not well its cool thanks anyway

chong · 10-04-2008, 08:53 PM

Congratulations, Scot!!!!

TomK · 10-04-2008, 09:19 PM

Very cool. Looking forward to watching your progress as I slowly accumulate my lab.

Gabe15 · 10-04-2008, 09:28 PM

Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare.

Chironex · 10-04-2008, 09:33 PM

Quote:

Originally Posted by Gabe15

Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare.

Good advice, Gabe. Will do. I set up some petri dishes with M&S medium in them just for that purpose since reading your post.

Chironex · 10-04-2008, 11:01 PM

OMG, I just roasted some petri dishes. Check out my gallery. Well now I know.

JCDerrick · 10-05-2008, 01:30 AM

LOL, I saw those at the top of the forum and was like "Uh oh"

Your set up looks great, I wish I had the time to try my hand at TC's. For now I'll have to settle for the pups.

Chironex · 10-05-2008, 07:13 PM

Gabe, the agar is clear so far, so I am hoping to have aseptic conditions in the hood. Wish me luck!!! I just went ahead and did 5 seeds for embryo rescue - man those little buggers are hard to hold and see. Hopefully the embryos weren't mangled too much. We will have to see if anything grows besides contamination. I tried to be sooooo careful about that, but it's a crap shoot.
If they get something going I will post some pics. I placed each GA7 jar into a zip-loc baggie afterwards (done in the hood) so I am hoping to avoid contamination there, too. I hope at least one of them is a good rescue, but they are so difficult to see, much less to slice the seed. The first one, the scalpel blade broke and the seed went flying. I found it, resterilized everything and tried again, but the embryo looked rotten. It was all brown - perhaps oxidized from leaving the seeds in 10% bleach solution overnight! Oops!
How do you hold onto the seeds and cut them? I found it very difficult using forceps and a surgical scalpel. Is there another, easier way to do this? When you do ER, do you use a macroscope to see better? I was thinking of doing that, but after the difficulty cutting the seed, there is no way I can imagine doing this on a macroscope stage without knocking it over. Maybe one of the reverse viewing types with the stage as part of the base????
Anyway, the first cultures are going now. I have a Saba corm that's not doing anything after 3-4 weeks, so I might grab it to TC it. It has one more week to show me signs of life, or it's going under the knife!!!

mskitty38583 · 10-06-2008, 10:04 AM

congrats scot! im not that ambitious yet.....i want to try growing from seed first but i have a lot more to learn before i even start that. congrats again!!!!!

Tog Tan · 10-06-2008, 12:12 PM

Way to go Scot! This is something I have always been dreaming about, now that you are onto to it, its gonna be 'look n learn' from your updates. All the best mate.

MediaHound · 10-06-2008, 12:42 PM

Yea, way to go! Sounds awesome!

Bananaman88 · 10-06-2008, 01:48 PM

Good luck, Scot! Sounds like you're having fun now!

Bob · 10-06-2008, 02:14 PM

Sounds really cool, once you have it down the sky's the limit I guess. The petri dishes look like Frankenstein got to them though!

Gabe15 · 10-10-2008, 04:09 AM

Perhaps you had seen this post awhile back, but this was my first couple attempts at embryo rescue, I also briefly detail some tips on opening the seeds.

Germination the easy way

Chironex · 10-10-2008, 04:10 AM

Yes, I saw and learned from it. Thank you Gabe.

Chironex · 10-21-2008, 02:50 AM

Just put Manzano and another cold hardy banana into culture. The Manzano was last week, the cold hardy banana this evening. The one this evening was my best apical meristem dissection yet. It was textbook, except the media was still a bit soft when I put it in, so it sunk in a bit deeper than I wanted it, but that means more exposure to the media.
I was going to TC the Saba corm, but as I was lifting it out of the pot, I noticed that roots were growing like crazy and pups were forming, so I left it alone.
I will have some pictures tomorrow in my gallery. My camera isn't that great, so the resolution is so so. But a couple turned out ok.
The Manzano explant is elongating and no signs of contamination.
As for the Embryo rescue, despite being on the wrong media, it looks like there are some changes going on with one of the cultures, so I am optimistic that at least one will take. (cross your fingers!)

10-04-2008, 08:40 PM	#1 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,155 BananaBucks : 448 Feedback: 5 / 100% Thanks: 1,771 Thanked 300 Times in 209 Posts Welcomes: 244	My home TC lab is open, Finally! Just finished setting up a few things and starting my first culture today, an embryo rescue is first on my agenda. The flow hood is in my photo gallery along with some pics of the recently received starts. Will continue to post progress reports as more gets going. __________________ Scot "Grow 'em if ya got 'em!" Last edited by Chironex : 10-04-2008 at 10:59 PM.

10-04-2008, 08:43 PM	#2 (permalink)
xavierdlc61887 xavierdlc61887's Photo Gallery Location: Texas Find me on the map! Zone: 10 Name: Fernie Join Date: Sep 2007 Posts: 472 BananaBucks : 9,846 Feedback: 14 / 100% Thanks: 60 Thanked 57 Times in 49 Posts Welcomes: 31	Re: My home TC lab is open, Finally! nice man hey did u ever send the seeds cause i never got anything...if not well its cool thanks anyway

10-04-2008, 08:53 PM	#3 (permalink)
chong Senior Member chong's Photo Gallery Location: Seattle, WA Find me on the map! Zone: 8-9 Name: Chong Join Date: Nov 2006 Posts: 916 BananaBucks : 6,507 Feedback: 3 / 100% Thanks: 163 Thanked 370 Times in 218 Posts Welcomes: 225	Re: My home TC lab is open, Finally! Congratulations, Scot!!!!

10-04-2008, 09:19 PM	#4 (permalink)
TomK Location: Boca Raton, Florida Find me on the map! Zone: 10 Name: Tom Join Date: Jul 2008 Posts: 20 BananaBucks : 612 Feedback: 0 / 0% Thanks: 5 Thanked 9 Times in 9 Posts Welcomes: 9	Re: My home TC lab is open, Finally! Very cool. Looking forward to watching your progress as I slowly accumulate my lab.

10-04-2008, 09:28 PM	#5 (permalink)
Gabe15 MC Banana Commander Gabe15's Photo Gallery Location: Honolulu, HI Find me on the map! Zone: 11 Join Date: Jul 2005 Posts: 1,536 BananaBucks : 16,388 Feedback: 4 / 100% Thanks: 1 Thanked 767 Times in 322 Posts Welcomes: 1	Re: My home TC lab is open, Finally! Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare. __________________ The only hemp Im growing is Manila.

Members currently in the chatroom: 0
	The most chatters online in one day was 14, 11-13-2007. No one is currently using the chat.

10-04-2008, 11:01 PM	#7 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,155 BananaBucks : 448 Feedback: 5 / 100% Thanks: 1,771 Thanked 300 Times in 209 Posts Welcomes: 244	Re: My home TC lab is open, Finally! OMG, I just roasted some petri dishes. Check out my gallery. Well now I know. __________________ Scot "Grow 'em if ya got 'em!"

10-05-2008, 01:30 AM	#8 (permalink)
JCDerrick KeoniKale JCDerrick's Photo Gallery Location: Lexington, SC Find me on the map! Zone: 8b Name: John Join Date: Sep 2008 Posts: 229 BananaBucks : 2,923 Feedback: 2 / 100% Thanks: 57 Thanked 48 Times in 43 Posts Welcomes: 8	Re: My home TC lab is open, Finally! LOL, I saw those at the top of the forum and was like "Uh oh" Your set up looks great, I wish I had the time to try my hand at TC's. For now I'll have to settle for the pups. __________________ Green fingers are the extension of a verdant soul.

10-05-2008, 07:13 PM	#9 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,155 BananaBucks : 448 Feedback: 5 / 100% Thanks: 1,771 Thanked 300 Times in 209 Posts Welcomes: 244	Re: My home TC lab is open, Finally! Gabe, the agar is clear so far, so I am hoping to have aseptic conditions in the hood. Wish me luck!!! I just went ahead and did 5 seeds for embryo rescue - man those little buggers are hard to hold and see. Hopefully the embryos weren't mangled too much. We will have to see if anything grows besides contamination. I tried to be sooooo careful about that, but it's a crap shoot. If they get something going I will post some pics. I placed each GA7 jar into a zip-loc baggie afterwards (done in the hood) so I am hoping to avoid contamination there, too. I hope at least one of them is a good rescue, but they are so difficult to see, much less to slice the seed. The first one, the scalpel blade broke and the seed went flying. I found it, resterilized everything and tried again, but the embryo looked rotten. It was all brown - perhaps oxidized from leaving the seeds in 10% bleach solution overnight! Oops! How do you hold onto the seeds and cut them? I found it very difficult using forceps and a surgical scalpel. Is there another, easier way to do this? When you do ER, do you use a macroscope to see better? I was thinking of doing that, but after the difficulty cutting the seed, there is no way I can imagine doing this on a macroscope stage without knocking it over. Maybe one of the reverse viewing types with the stage as part of the base???? Anyway, the first cultures are going now. I have a Saba corm that's not doing anything after 3-4 weeks, so I might grab it to TC it. It has one more week to show me signs of life, or it's going under the knife!!! __________________ Scot "Grow 'em if ya got 'em!" Last edited by Chironex : 10-05-2008 at 07:20 PM. Reason: added

10-06-2008, 10:04 AM	#10 (permalink)
mskitty38583 banana junkie mskitty38583 's Photo Gallery Location: sparta, tn. Find me on the map! Zone: 6-7 Name: mskitty Join Date: Nov 2007 Posts: 3,598 BananaBucks : 16,506 Feedback: 24 / 100% Thanks: 23 Thanked 462 Times in 393 Posts Welcomes: 59	Re: My home TC lab is open, Finally! congrats scot! im not that ambitious yet.....i want to try growing from seed first but i have a lot more to learn before i even start that. congrats again!!!!! __________________ WELCOME TO THE GATOR NATION "kokomo" beach boys:

10-06-2008, 12:12 PM	#11 (permalink)
Tog Tan Ensete nut Tog Tan's Photo Gallery Location: Kuala Lumpur, Malaysia Name: Tog Join Date: Jul 2008 Posts: 157 BananaBucks : 1,369 Feedback: 0 / 0% Thanks: 9 Thanked 52 Times in 26 Posts Welcomes: 24	Re: My home TC lab is open, Finally! Way to go Scot! This is something I have always been dreaming about, now that you are onto to it, its gonna be 'look n learn' from your updates. All the best mate. __________________

10-06-2008, 12:42 PM	#12 (permalink)
MediaHound Tally-man MediaHound's Photo Gallery Location: South Florida Find me on the map! Zone: 10b Name: Jarred Join Date: Jul 2005 Posts: 4,020 BananaBucks : 53,477 Feedback: 58 / 100% Thanks: 927 Thanked 1,098 Times in 382 Posts Welcomes: 243	Re: My home TC lab is open, Finally! Yea, way to go! Sounds awesome!

10-06-2008, 01:48 PM	#13 (permalink)
Bananaman88 Bananaculturist Bananaman88's Photo Gallery Location: Pearland, TX Find me on the map! Zone: 9 Name: Brent Join Date: Jun 2007 Posts: 1,092 BananaBucks : 8,493 Feedback: 15 / 100% Thanks: 112 Thanked 302 Times in 207 Posts Welcomes: 8	Re: My home TC lab is open, Finally! Good luck, Scot! Sounds like you're having fun now!

10-06-2008, 02:14 PM	#14 (permalink)
Bob Location: Morris plains N.J. Find me on the map! Zone: 6a Name: bob Join Date: Sep 2008 Posts: 179 BananaBucks : 1,828 Feedback: 3 / 100% Thanks: 191 Thanked 51 Times in 41 Posts Welcomes: 51	Re: My home TC lab is open, Finally! Sounds really cool, once you have it down the sky's the limit I guess. The petri dishes look like Frankenstein got to them though!

10-10-2008, 04:09 AM	#15 (permalink)
Gabe15 MC Banana Commander Gabe15's Photo Gallery Location: Honolulu, HI Find me on the map! Zone: 11 Join Date: Jul 2005 Posts: 1,536 BananaBucks : 16,388 Feedback: 4 / 100% Thanks: 1 Thanked 767 Times in 322 Posts Welcomes: 1	Re: My home TC lab is open, Finally! Perhaps you had seen this post awhile back, but this was my first couple attempts at embryo rescue, I also briefly detail some tips on opening the seeds. Germination the easy way __________________ The only hemp Im growing is Manila.

10-10-2008, 04:10 AM	#16 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,155 BananaBucks : 448 Feedback: 5 / 100% Thanks: 1,771 Thanked 300 Times in 209 Posts Welcomes: 244	Re: My home TC lab is open, Finally! Yes, I saw and learned from it. Thank you Gabe. __________________ Scot "Grow 'em if ya got 'em!"

10-21-2008, 02:50 AM	#17 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,155 BananaBucks : 448 Feedback: 5 / 100% Thanks: 1,771 Thanked 300 Times in 209 Posts Welcomes: 244	Re: My home TC lab is open, Finally! Just put Manzano and another cold hardy banana into culture. The Manzano was last week, the cold hardy banana this evening. The one this evening was my best apical meristem dissection yet. It was textbook, except the media was still a bit soft when I put it in, so it sunk in a bit deeper than I wanted it, but that means more exposure to the media. I was going to TC the Saba corm, but as I was lifting it out of the pot, I noticed that roots were growing like crazy and pups were forming, so I left it alone. I will have some pictures tomorrow in my gallery. My camera isn't that great, so the resolution is so so. But a couple turned out ok. The Manzano explant is elongating and no signs of contamination. As for the Embryo rescue, despite being on the wrong media, it looks like there are some changes going on with one of the cultures, so I am optimistic that at least one will take. (cross your fingers!) __________________ Scot "Grow 'em if ya got 'em!"

10-21-2008, 12:57 PM	#18 (permalink)
Bananaman88 Bananaculturist