Bananas.org

Welcome to the Bananas.org forums.

You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today!

If you have any problems with the registration process or your account login, please contact us.

Go Back   Bananas.org > Banana Forum > Tissue Culturing & Other Propagation Techniques of Banana Plants
The Facebook Platform
Register Photo Gallery Classifieds Wiki Chat Map Search Today's Posts Mark Forums Read

Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


Members currently in the chatroom: 0
The most chatters online in one day was 17, 09-06-2009.
No one is currently using the chat.

Reply   Email this Page Email this Page
 
LinkBack Thread Tools
Old 10-22-2008, 01:22 AM   #21 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

I have to say, I am having a blast doing this so far. I keep a journal to log all of the details, so if I make a mistake, it is noted. (Like roasting the 2 culture jars in the pressure cooker!!! LOL)

It is amazing to me that that tiny piece of apical meristem can create so many banana plants. After I feel like i have it down pat, I am going to attempt to induce some mutant variegates.

Thank you for all of your kind words and support.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 10-22-2008, 08:49 AM   #22 (permalink)
Moderator

 
bigdog's Avatar
 
Location: Smyrna, TN
Zone: 7a
Name: Frank
Join Date: Aug 2005
Posts: 1,986
BananaBucks : 474,303
Feedback: 7 / 100%
Said "Thanks" 195 Times
Was Thanked 1,630 Times in 455 Posts
Said "Welcome to Bananas" 131 Times
Send a message via AIM to bigdog Send a message via MSN to bigdog
Default Re: My home TC lab is open, Finally!

Scot, I've found the best way to rescue embryos from Musa seeds is with a small vice, mounted on a table. You just slowly tighten the vice until you hear a crack, then remove the seed from the vice. Find the crack, and pry it open with a scalpel, knife, something sharp. I haven't yet mangled the first embryo with this method.

Regarding contamination...it really is ridicuously easy to grow mold, lol. We did some cultures the other day, and 3 out of our 9 cultures got some contamination. I didn't feel so bad though, seeing the next group get every single one of theirs contaminated! This was with a professional setup, but most of the students had very little or no tc experience. Even with a constant flame, wiping down with ethyl alcohol, 10% bleach soak, rinsing with distilled water many times, and working as close to the hood as possible, contamination is still possible. It only takes one little particle! Good luck with your setup there.

Frank
bigdog is offline   Reply With Quote Send A Private Message To bigdog
Said thanks:
Old 10-23-2008, 05:41 PM   #23 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Quote:
Originally Posted by bigdog View Post
Scot, I've found the best way to rescue embryos from Musa seeds is with a small vice, mounted on a table. You just slowly tighten the vice until you hear a crack, then remove the seed from the vice. Find the crack, and pry it open with a scalpel, knife, something sharp. I haven't yet mangled the first embryo with this method.

Regarding contamination...it really is ridicuously easy to grow mold, lol. We did some cultures the other day, and 3 out of our 9 cultures got some contamination. I didn't feel so bad though, seeing the next group get every single one of theirs contaminated! This was with a professional setup, but most of the students had very little or no tc experience. Even with a constant flame, wiping down with ethyl alcohol, 10% bleach soak, rinsing with distilled water many times, and working as close to the hood as possible, contamination is still possible. It only takes one little particle! Good luck with your setup there.

Frank
Thanks! I think I will buy a C - clamp to do the same thing. That way I can sterilze it in the pressure cooker, wipe it with alcohol and put in in the hood to crack the seeds. I try to do as much under the hood as I can.
So far, only one contaminated out of 7 cultures. It was one of the Ingens embryo rescues, and we know that they seem to be likely to have endocarpic contaminates, no matter how much I sterilize them. But, I will say that out of the 5 I did, 1 or 2 look like there is some promise. The other 2 have no contamination, but are doing nothing, so I may have injured the embryo upon removal. The 5th one was the contaminated.

The Manzano and the California Cold hardy banana cultures appear to be aseptic so far. I will be doing 2 more cultures tomorrow or this weekend.

I appreciate your encouragement.
__________________
Scot


Click for Jakarta, Indonesia Forecast

Last edited by Chironex : 10-23-2008 at 05:43 PM. Reason: spellin' an spacin'
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 10-23-2008, 05:53 PM   #24 (permalink)
Retired.
 
Richard's Avatar
 
Location: Vista, CA
Zone: USDA 10b
Name: Richard
Join Date: Jan 2008
Posts: 9,211
BananaBucks : 1,360
Feedback: 9 / 100%
Said "Thanks" 3,291 Times
Was Thanked 11,292 Times in 4,328 Posts
Said "Welcome to Bananas" 1,667 Times
Default Re: My home TC lab is open, Finally!

Go Scot, go! I see from your avatar that your cortex stem has gone banana.
__________________


8 million banana bucks and counting.
Frequently found at GrowingFruit.org
Richard is offline   Reply With Quote Send A Private Message To Richard
Said thanks:
Old 10-23-2008, 05:58 PM   #25 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Quote:
Originally Posted by Richard View Post
Go Scot, go! I see from your avatar that your cortex stem has gone banana.
The way I see it, if Jimmy Buffett can have Parrotheads, I can have BananaHeads, right?
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 10-23-2008, 08:22 PM   #26 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Here is one of 3 pics posted in my gallery of a Manzano TC. It is beginning to shoot now.

__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 10-23-2008, 11:03 PM   #27 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Here is one that was just put into TC today for one of our members. The surgery went well, and the prognosis is excellent.

__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 10-24-2008, 10:14 AM   #28 (permalink)
Living in Exile
 
damaclese's Avatar
 
Location: Henderson NV
Zone: 9 Mediterranean climate
Name: Paulo
Join Date: Apr 2008
Posts: 1,767
BananaBucks : 215,756
Feedback: 0 / 0%
Said "Thanks" 1,352 Times
Was Thanked 1,615 Times in 703 Posts
Said "Welcome to Bananas" 101 Times
Default Re: My home TC lab is open, Finally!

Scot you are so cool dude thanks for sharing this with us congrats looking forwerd to more info and pics keep them coming! from the pics they defanatly look good to me! how are the Engen seeds doing? can't wate till you can Tc those im sure you will have no problims selling them to the Org rember im in line to LOL
__________________
Helping to foster understanding for the learning disabled

damaclese is offline   Reply With Quote Send A Private Message To damaclese
Said thanks:
Old 10-24-2008, 12:20 PM   #29 (permalink)
Been nuts, gone bananas
 
harveyc's Avatar
 
Location: Isleton, Calif
Zone: 9b
Name: Harvey
Join Date: May 2007
Posts: 5,101
BananaBucks : 48,731
Feedback: 5 / 100%
Said "Thanks" 5,982 Times
Was Thanked 4,321 Times in 1,851 Posts
Said "Welcome to Bananas" 1,784 Times
Default Re: My home TC lab is open, Finally!

Scot, thanks for all the photos, comments, etc.

Whey you refer to "California Cold Hardy", are you referring to the one sold by Shawn in Lodi, CA? Or is there something else referred to as that?

Harvey
__________________
harveyc is offline   Reply With Quote Send A Private Message To harveyc
Said thanks:
Old 10-24-2008, 02:36 PM   #30 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Quote:
Originally Posted by harveyc View Post
Scot, thanks for all the photos, comments, etc.

Whey you refer to "California Cold Hardy", are you referring to the one sold by Shawn in Lodi, CA? Or is there something else referred to as that?

Harvey
Yes, I believe that is the one. Thanks for your kind remarks.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 10-25-2008, 06:15 AM   #31 (permalink)
Bob
Orang Puteh
 
Bob's Avatar
 
Location: Morris plains N.J.
Zone: 6a
Name: bob
Join Date: Sep 2008
Posts: 3,507
BananaBucks : 150,100
Feedback: 12 / 100%
Said "Thanks" 9,472 Times
Was Thanked 3,984 Times in 1,668 Posts
Said "Welcome to Bananas" 562 Times
Default Re: My home TC lab is open, Finally!

Quote:
Originally Posted by damaclese View Post
Scot you are so cool dude thanks for sharing this with us congrats looking forwerd to more info and pics keep them coming! from the pics they defanatly look good to me! how are the Engen seeds doing? can't wate till you can Tc those im sure you will have no problims selling them to the Org rember im in line to LOL
Ditto for me.
__________________
Click for Morris Plains, New Jersey Forecast
Bob is offline   Reply With Quote Send A Private Message To Bob
Said thanks:

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 10-25-2008, 09:30 AM   #32 (permalink)
gopher
 
ursula's Avatar
 
Location: va
Zone: 7
Name: ursula
Join Date: Oct 2008
Posts: 17
BananaBucks : 2,017
Feedback: 0 / 0%
Said "Thanks" 7 Times
Was Thanked 2 Times in 2 Posts
Said "Welcome to Bananas" 7 Times
Default Re: My home TC lab is open, Finally!

with all the experience you all have you make me feel very insecure!
cant you discuss technical theatre for a while?
ursula
ursula is offline   Reply With Quote Send A Private Message To ursula
Said thanks:
Old 10-25-2008, 10:34 AM   #33 (permalink)
Tally-Man

 
MediaHound's Avatar
 
Location: Davie, Florida
Zone: 10
Name: Jarred
Join Date: Jul 2005
Posts: 5,241
BananaBucks : 1,180,470
Feedback: 66 / 100%
Said "Thanks" 3,771 Times
Was Thanked 4,583 Times in 1,233 Posts
Said "Welcome to Bananas" 2,059 Times
Default Re: My home TC lab is open, Finally!

Good work Scot!
__________________
Apologies in advance if I am slow to reply to your PM. I suggest posting in the forums for support if you need something urgent.
MediaHound is offline   Reply With Quote Send A Private Message To MediaHound
Said thanks:
Old 10-25-2008, 07:00 PM   #34 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Today put 6 E. perrieri embryos into culture along with 1 laterita and 1 itinerans embryo. Also took one ingens seed from germination tray and rescued the embryo onto culture media. Gabe was very helpful while I was doing this and Frank's idea of using a small vise to crack the seed hull worked like a dream! It's the only way to go from now on. Look how small an itinerans seed is and imagine trying to split it without damaging an embryo the size of a pin head! Using the vise, the embryos just fell out, or were easily removed if they were stubborn. Note: this method works best on dry seeds, if soaked, they just split open slightly. Embryos were easier to extract from the dry seeds.

We will see how they look as time passes. No pic's yet, my camera resolution is not that great, so they would be blurred, if visible at all.
Seeds were first sterilized in Physan 20 with some Tween 20 as a surfactant. Then they were rinsed for 20 minutes in 10% bleach water solution. The seeds were then cracked, as aseptically as possible, and put into a quick rinse in 10% bleach solution, rinsed 3 times in sterile water, then placed upright onto M&S media.
I hope to get my hands on some better media for embryo rescue at some point, but this was all that was available for now.
Now we wait...
__________________
Scot


Click for Jakarta, Indonesia Forecast

Last edited by Chironex : 10-25-2008 at 07:03 PM. Reason: edited for television
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 10-25-2008, 07:04 PM   #35 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Quote:
Originally Posted by ursula View Post
with all the experience you all have you make me feel very insecure!
cant you discuss technical theatre for a while?
ursula
Technical theatre - ummm, project your voice from the diaphram, that's the extent of my knowledge - you got me there. lol
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Sponsors

Old 10-26-2008, 12:59 PM   #36 (permalink)
many 'naners, little time
 
51st state's Avatar
 
Location: salisbury, UK
Zone: 8b ish
Name: Kev
Join Date: Nov 2006
Posts: 465
BananaBucks : 195,876
Feedback: 3 / 100%
Said "Thanks" 202 Times
Was Thanked 245 Times in 119 Posts
Said "Welcome to Bananas" 0 Times
Default Re: My home TC lab is open, Finally!

Looking good there Scot. keep it up.

Once you've got it nailed I'll try get some unusual stuff over to you
__________________
a>
51st state is offline   Reply With Quote Send A Private Message To 51st state
Said thanks:
Old 10-26-2008, 01:12 PM   #37 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Kev, that would be fun. Wait until I get more trials in with different media. Some variances occur with different banana varieties. Everything is being logged so that the formulas that work on certain bananas will already be known. Need to get the medium for embryo rescue, as the normal M&S is not as robust.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 11-20-2008, 04:27 AM   #38 (permalink)
 
Join Date: May 2008
Posts: 45
BananaBucks : 13,167
Feedback: 0 / 0%
Said "Thanks" 1 Times
Was Thanked 10 Times in 7 Posts
Said "Welcome to Bananas" 0 Times
Default Re: My home TC lab is open, Finally!

Wow that's so cool :-) Good luck with it.
edd82 is offline   Reply With Quote Send A Private Message To edd82
Said thanks:
Old 12-25-2008, 03:01 PM   #39 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,757
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: My home TC lab is open, Finally!

Yesterday 8 more ingens embryos were dissected and placed into embryo rescue. Still waiting for chemicals to use with the new medium using modified Nitsch & Nitsch.

The Viente Cohol was sub-divided again into 12 plantlets. The California Cold-hardy banana has not done anything since induction. Can't figure out why, as there does not appear to be any contamination. It just seems to be happy taking its time.

The Manzano culture is also taking its sweet time to do anything. Next cultures I do I will split up using charcoal in the medium and citrate. I have also been doing some reading about alternative media such as straight peat with some hormones. Others are cotton balls, perlite, vermiculite and gellan gum. I got some of each to test the differences in cultures.

Phytotech Labs has a Banana culture medium that also shows some promise, so I am going to set-up some trials with it, too.

The hope is to aggregate banana TC data and construct a "best culture environment" database for each banana type, including Ensete and Musella. Sometimes the strength of the medium including the additives can vary the results dramatically.

There is also an antibiotic that has been shown to induce variegation that remains true to type. This may be a key to use in AeAe culturing.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Said thanks:
Old 12-26-2008, 12:32 PM   #40 (permalink)
Been nuts, gone bananas
 
harveyc's Avatar
 
Location: Isleton, Calif
Zone: 9b
Name: Harvey
Join Date: May 2007
Posts: 5,101
BananaBucks : 48,731
Feedback: 5 / 100%
Said "Thanks" 5,982 Times
Was Thanked 4,321 Times in 1,851 Posts
Said "Welcome to Bananas" 1,784 Times
Default Re: My home TC lab is open, Finally!

Wow, Scot, you've really got into this! What are your objectives in producing plants? Are these for your own use, to give away to friends and family, or do you plan on selling plants?

While I have no plans to for commercial applications, I thought I might like to learn TC so that I could produce more plants for my own use. For instance, a fast maturing variety like Viente Cohol might work well being grown as an annual so I wouldn't have to worry about cold hardiness. I'm thinking I could TC some plants in the fall or winter and grow them in pots in my greenhouse until spring and hope for ripe fruit by the end of summer or early fall from several plants. I have lots of relatives and friends to share the fruit with.

I'm not sure I have the discipline necessary to carry out the TC process, though. I just need to focus my attention, perhaps.

Thanks for sharing!
__________________
harveyc is offline   Reply With Quote Send A Private Message To harveyc
Said thanks:
Reply   Email this Page Email this Page

Previous Thread: tissue culture kits
Next Thread: Then and now.





Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools



All times are GMT -5. The time now is 11:52 PM.





Follow us:
Twitter YouTube

All content © Bananas.org & the respective author.