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-   -   Multiplication in TC (http://www.bananas.org/f260/multiplication-tc-22968.html)

fortuemuf 12-30-2015 07:44 AM

Multiplication in TC
 
Hi
l am going bananas, l have been trying to culture Williams and dwarfs and all l get is a single shoot. How do l make my initiates multiply-media suggestions that work?

Gabe15 12-30-2015 04:30 PM

Re: Multiplication in TC
 
What is your medium formulation, especially hormones?

Normally for shoot elongation very little or no BA is present, but for multiplication you need to have BA present in high quantity. In the lab I used to work at, for shoot proliferation we would use 1ml/L of 4mg/ml BA solution in the medium. You can multiply on this medium for up to 5 subculture cycles, at which point everything should be transferred to a medium with no BA and should elongate and root. After 5 cycles, you increase the risk perpetuating undesirable off-type somatic mutations, so you should restart with healthy, normal field grown stock.

siege2050 12-30-2015 11:27 PM

Re: Multiplication in TC
 
While we are on the subject, can anyone recommend a decent kit etc. to learn how to TC? What is the best way to learn?

fortuemuf 12-31-2015 01:43 AM

Re: Multiplication in TC
 
Thanks a million , have recently bought BAP and will be trying it, will keep you posted on the results

Gabe15 01-08-2016 12:30 AM

Re: Multiplication in TC
 
Quote:

Originally Posted by siege2050 (Post 267547)
While we are on the subject, can anyone recommend a decent kit etc. to learn how to TC? What is the best way to learn?

A lot of resources here: Home - KITCHEN CULTURE KITS INC

fortuemuf 04-10-2017 06:57 AM

Re: Multiplication in TC
 
Am still to get a break through, had stopped but started again today so will be posting progress and challenges


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