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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 10-23-2013, 06:23 PM   #1 (permalink)
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Joy I just might try my hand at TCing. Advice?

Hey everyone. I've been interested in TCing for a while now, and now I just might have the opportunity to try it. I'm looking at purchasing a basic kit from Kitchen Culture Kits, and then propagating a few hundred banana plants, mostly for either donation to low income families through the local food bank or for selling at the campus garden's weekly garden sale. In particular, I'm looking to TC Misi Luki Namwah, Manzano, Orinoco, Blue Java (the real deal), and possibly Pisang Klotek. I have a few questions:
  • I am on a budget. Is there a cheap way I can prepare a clean space so that I can minimize contamination?
  • The Kitchen Culture Kit comes with a few plant hormones, but it's designed for African Violet plants. Will these hormones work fine on banana plants too? (In particular it says that BAP induces shoot formation in African Violets.)
  • Any other tips on minimizing contamination? I feel like this would be the biggest issue that I would have, especially given that I'm going to be working in a space that has a lot of other plants around it, particularly seedlings, as well as honey bees.
  • I will be TCing in a large unheated greenhouse, most likely. Here, generally speaking, during the winter time the lowest temperature that the greenhouse gets (I think) is in the 40s at night. During the day it will get to be in the 80s and 90s. Would this be too cold and/or hot for the plantlets? I could bring them to a more temperate space when I am waiting for them to propagate. Or possibly insulate them.

Any advice is greatly appreciated. I will probably have more questions later so stay tuned!
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Old 10-24-2013, 02:07 AM   #2 (permalink)
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Default Re: I just might try my hand at TCing. Advice?

I am curious about this stuff as well.
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Old 10-24-2013, 02:13 PM   #3 (permalink)
 
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Default Re: I just might try my hand at TCing. Advice?

You will need a sterile transfer hood. I once used a plastic box with holes cut in the lid so I could put my arms in. It will need to be sterilised with bleach and ethanol before and after each use.
The hormones supplied with your kit would be fine.
Greenhouse is too hot/cold they need to be kept at an even temperature around 22 degrees under lights.
You really need to read all the posts on TC in the forum, and I mean all of them, there is no short cut. They are all very good.
I tried it but was never any good at it. It's very difficult and time consuming to do it properly.
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Old 10-24-2013, 06:26 PM   #4 (permalink)
 
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Default Re: I just might try my hand at TCing. Advice?

Quote:
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Greenhouse is too hot/cold they need to be kept at an even temperature around 22 degrees under lights.
Heck, I don't think I could hold that temp in the barn up in Ohio when the snow fell as it did quite often. Did you really mean 22 degrees? If my kitchen was ever that cold...well...it just would never be.
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Old 10-24-2013, 06:27 PM   #5 (permalink)
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Default Re: I just might try my hand at TCing. Advice?

I'll try and find a way to keep them warm during the night then. The temperature of the greenhouse can be somewhat adjusted by opening and closing the ventilation. Looks like I'll also need grow lights. Thanks for the advice!
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Old 10-24-2013, 06:28 PM   #6 (permalink)
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Default Re: I just might try my hand at TCing. Advice?

Quote:
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Heck, I don't think I could hold that temp in the barn up in Ohio when the snow fell as it did quite often. Did you really mean 22 degrees?
22 degrees Celsius, which is around 72 F.
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Old 10-24-2013, 06:44 PM   #7 (permalink)
 
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Default Re: I just might try my hand at TCing. Advice?

Quote:
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22 degrees Celsius, which is around 72 F.
How a letter changes things! 72 F is doable--never had my thermostat above 65 F up north ever--layers are your friend there--inside or outside.
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Old 11-25-2013, 08:10 AM   #8 (permalink)
 
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Default Re: I just might try my hand at TCing. Advice?

it is really very nice and informative forum ,thanks for sharing .i really appreciate this ....
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Old 01-27-2015, 08:21 PM   #9 (permalink)
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Default Re: I just might try my hand at TCing. Advice?

Since I'm new here, perhaps someone could point me to a thread that discusses how to do TCing; exactly what it is, how it all works, etc? Many thanks, in advance.
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Old 01-27-2015, 08:34 PM   #10 (permalink)
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Default Re: I just might try my hand at TCing. Advice?

I am thinking the best way would be to buy a kit to learn with, I am fixing to purchase one, I dont know if the one in the link below is good or not but there are a few online to purchase. It would be easier than trying to guess what you need. After you learn how to to it, then you would know what to use later on your own.

http://youtu.be/nRXU_AWmRU4
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Last edited by siege2050 : 01-27-2015 at 08:36 PM.
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