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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 09-05-2018, 03:18 PM   #1 (permalink)
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Default First Macropropagation experiments - Sddarkman619

So I'm giving this a shot as well. There are some plants that I've been wanting to propagate and sell for a while so that people get what they are buying and not some misnamed plants, namely Blue Java and a few others.
The plants in this post are all blue java acquired from a reputable source on here. I had donated 1 blue java plant to my daughters school as I had no room to plant more on my property, and I liked the idea of having a "Plant Bank" I could draw from. The 1 plant turned into a mat of about 15 plants. Some fruit was growing but it needed thinning.
So I spent a few hours one day thinning and culling nice corms and pups for my experiments.
Some of the corms probably won't make it as they are probably too small and have actually been killed. The larger ones seem to be doing ok and I had left roots on those and effectively killed the merristem so that shoots would form off the side of the damaged corms. These are in soil and are doing well as you will see.

A new corm was taken today, pared down and cleaned. It is now being "Seasoned", left out in the air to dry the other layer for 2 days. I will then soak it in BAP and then plant in a bin of sawdust. I cut some buds off of this corm and am soaking them in BAP solution and will plant these in soil for more plants.

The monster mat I had to thin:


the fruit:


corms for growing and experiments in Macropropagation:


after thinning. After checking on these after 2 weeks the fruit had doubled in size and one plant put out a new flower:


one of the soil grown corms that had effectively been "killed" to produce shoots form the side, there are 3 shoots after 2 weeks and the circle is the damaged top of the corm. This corm I left some root shoots on it when planting:


another one that was showing merristem growth. Happy to know it was alive and well, I "killed" the merristem to initiate side shoots:


Same thing with above here:


Plants for future experiments, all blue javas:


The temperature controlled bin with 3 small corms that are doing nothing after 3 weeks:


bin with temp sensor in the sawdust to thermostat for controlling temps:


My new corm which is a nice size, pared down and effectively killing the merristem to initiate side shoots. Currently being "Seasoned" by leaving it out for 2 days to dry the outer layer:






The buds pared off the new corm, soaking in a solution of BAP and other nutrients, will be planted later today to grow new plants for further abuse:
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Old 09-05-2018, 09:02 PM   #2 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Not so long ago in a place not to far away ... circa 2007 in Penasquitos ... I was purchasing TC's by the tray and growing them out in 1 gallon, then 5 gallon, and the few that didn't sell, to 15 gallon. I noticed that if I didn't stay on top of upsizing the pots the individual would get cramped and the rhizomes would start forming corms, sending up pups.

Also I noticed that if I took a 2' pup from a full size plant and place it in a tall 15 gallon, its going to start pupping in a year. So I would transplant 2' pups from mother plants at the end of summer into 15gal pots. Then in the spring when they started putting on some growth, cut the pseudostem about 5 inches above the soil line. By summer, lots of pups would be present.
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Old 09-05-2018, 09:18 PM   #3 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Thanks Richard. Going to do many experiments. This may be one of them the crowding technique.
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Old 09-06-2018, 03:01 AM   #4 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Looks like a nice rack of Namwa.
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Old 09-06-2018, 10:25 AM   #5 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Quote:
Originally Posted by Juicy Bananas View Post
Looks like a nice rack of Namwa.
Those aren't Namwah, they are Blue Javas.
The base plant was received from a reputable member here who have the real Blue Javas, which is what these are.
Not Namwah.

Take another look:






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Old 09-06-2018, 11:45 AM   #6 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Hey Sddarkman,

Which woods can be used for the sawdust in propagation? Both you and Louis used sawdust and I saw that you asked Louis about soft vs. hard woods but I didn't see a definitive answer.

If I cleared a plot I would have oak (hard) and pine (soft) as the material to use as sawdust or mulch. Are these woods safe/suitable? I thought I would just run a few logs through a chipper/duster.

Thanks for any info!
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Old 09-06-2018, 12:42 PM   #7 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Quote:
Originally Posted by Akula View Post
Hey Sddarkman,

Which woods can be used for the sawdust in propagation? Both you and Louis used sawdust and I saw that you asked Louis about soft vs. hard woods but I didn't see a definitive answer.

If I cleared a plot I would have oak (hard) and pine (soft) as the material to use as sawdust or mulch. Are these woods safe/suitable? I thought I would just run a few logs through a chipper/duster.

Thanks for any info!
I honestly don't know the answer to that question. I'm going to use a white/soft wood this go around as the others were in a dark/hardwood last time.

Once I get some more corms going I'll be trying each every other time and see how they go.

All the videos I've watched and all the papers I've read, don't specify any type. They just say...sawdust. Or rice hulls.
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Old 09-07-2018, 10:34 AM   #8 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

I have rice hulls 4 sale
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Old 09-11-2018, 08:55 PM   #9 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

If you have it available in your area get rice hulls! For macro! For potting mix! For in ground backfill! For anything banana.

Perlite and ricehulls are the 2 best best things for Musa! You can’t go wrong!
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Old 09-13-2018, 11:27 AM   #10 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

So I got the rice hulls and popped the Corms in there, but what happened is that they are so light and air, they don't hold heat like the sawdust. Even with a heating mat they got too cold, so I'm going to mix some sawdust back into the hulls to hold more water and heat. Also noticed they don't hold moisture like the sawdust does. But this will be good for my soil mix.
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Old 09-13-2018, 11:40 AM   #11 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Ty, are you using parboiled rice hulls or from the feed store(bedding)?
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Old 10-13-2018, 10:25 PM   #12 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

I just may try this over the winter. Instead if sawdust I will try sand.
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Old 10-14-2018, 02:51 PM   #13 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

I’m using parboiled and just normal dry rice hulls. Not bedding. I have rice mills 2 hours away. They charge $10 for a truckload. Used to be free.

I will try to upload a few of my macro pics to my Flickr. I’m making a folder in Flickr named “macro”. I use normal pine sawdust. The more powdery the better.
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Old 10-17-2018, 11:06 AM   #14 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Update on the Macro experiment. It's going well. However the corm in the soil is producing bigger faster growing plants.
Plant in the pots:
These two were harvested for member last night:






This is my sawdust bin. The pups are coming but they are slower to grow I imagine from lack of nutrients, so I mixed in some long term release fertilizer into the sawdust. These pups in the sawdust are about as old as the ones from the soil I just harvested.





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Old 10-17-2018, 06:26 PM   #15 (permalink)
 
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Default Re: First Macropropagation experiments - Sddarkman619

Looking good!!! Thank you for the updates and great photos!
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Old 11-01-2018, 12:35 PM   #16 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Here is the bud that forms at the "V" of the leaf sheaths.
One would damage the Apical Merristem to get this to pup out and form more pups. off of this one point.


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Old 11-09-2018, 07:55 PM   #17 (permalink)
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Default Re: First Macropropagation experiments - Sddarkman619

Thanks to a member on this board, I now have a Raja Puri to torture! MAUAHAHAHAHAHAhahahahaha.....




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