Bananas.org

Welcome to the Bananas.org forums.

You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today!

If you have any problems with the registration process or your account login, please contact us.

Go Back   Bananas.org > Banana Forum > Tissue Culturing & Other Propagation Techniques of Banana Plants
Register Photo Gallery Classifieds Wiki Chat Map Search Today's Posts Mark Forums Read

Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


Members currently in the chatroom: 0
The most chatters online in one day was 17, 09-06-2009.
No one is currently using the chat.

Reply   Email this Page Email this Page
 
LinkBack Thread Tools
Old 08-27-2019, 05:49 PM   #21 (permalink)
 
mcfly's Avatar
 
Location: Northern California - East Bay
Zone: PST
Join Date: Aug 2018
Posts: 36
BananaBucks : 10,710
Feedback: 0 / 0%
Said "Thanks" 38 Times
Was Thanked 44 Times in 20 Posts
Said "Welcome to Bananas" 0 Times
Default Re: First Macropropagation experiments - Sddarkman619

Excellent writeup and pictures Sddarkman619.

What is your conclusion now after all your testing? Did you have better results using Coco noir than the sawdust?

I want to try this method as well. This last week I became incredibly fascinated with this method. After refining this method, I heard that you can get ~64 plants from one corm.

Thanks!

mcfly
__________________


Currently growing:
Basjoo
Blue Java "Ice Cream"

Recently started:
Black Thai
Saba
Mekong Giant
Ensete Glaucum “Snow Banana”
Variegated Florida - non AEAE
?Mystery banana?
Maybe Senorita
Maybe Namwa
Dwarf Brazillian
Red Zebrina
Ensete ventricosum 'Maurelii'
mcfly is offline   Reply With Quote Send A Private Message To mcfly
Old 08-27-2019, 07:27 PM   #22 (permalink)
 
mcfly's Avatar
 
Location: Northern California - East Bay
Zone: PST
Join Date: Aug 2018
Posts: 36
BananaBucks : 10,710
Feedback: 0 / 0%
Said "Thanks" 38 Times
Was Thanked 44 Times in 20 Posts
Said "Welcome to Bananas" 0 Times
Default Re: First Macropropagation experiments - Sddarkman619

Sddarkman619,

When you pared down the corm, did you cut an 'x' on each bud that you saw? I heard that helps to get more sprouts from that location.

mcfly
__________________


Currently growing:
Basjoo
Blue Java "Ice Cream"

Recently started:
Black Thai
Saba
Mekong Giant
Ensete Glaucum “Snow Banana”
Variegated Florida - non AEAE
?Mystery banana?
Maybe Senorita
Maybe Namwa
Dwarf Brazillian
Red Zebrina
Ensete ventricosum 'Maurelii'
mcfly is offline   Reply With Quote Send A Private Message To mcfly
Old 08-27-2019, 09:50 PM   #23 (permalink)
Banana Man
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 1,148
BananaBucks : 188,606
Feedback: 14 / 100%
Said "Thanks" 340 Times
Was Thanked 1,219 Times in 535 Posts
Said "Welcome to Bananas" 1 Times
Default Re: First Macropropagation experiments - Sddarkman619

I did have a nice outcome with the coco coir, much better than saw dust or rice hulls. For me at least.
Yes you have to basically kill/damage the bud down to it's merristem in order to get it to put pups out rather than 1 pseudostem.
I have now started using a drill bit instead. seeing how that works.
__________________
Ca Licensed Nursery - Snail/Disease Free and Inspected:
Bountiful Garden Nursery - A1469.001

Banana Plants for Sale
https://www.bananatreesforsale.com


Sign up to the Newsletter for Updates:
Sign Up Here
sddarkman619 is offline   Reply With Quote Send A Private Message To sddarkman619
Said thanks:
Old 08-30-2019, 07:07 AM   #24 (permalink)
Commercial Grower
 
PR-Giants's Avatar
 
Location: Florida & Greater Antilles
Join Date: Feb 2012
Posts: 4,544
BananaBucks : 2,436
Feedback: 16 / 100%
Said "Thanks" 2,900 Times
Was Thanked 12,461 Times in 3,620 Posts
Said "Welcome to Bananas" 3,204 Times
Default Re: First Macropropagation experiments - Sddarkman619

Quote:
Originally Posted by sddarkman619 View Post

Yes you have to basically kill/damage the bud down to it's merristem in order to get it to put pups out rather than 1 pseudostem.
Most propagation manuals will say to kill or damage the Growing Point in order to remove apical dominance. That's mainly because plants are plentiful and inexpensive and the technique is quick and easy, but obviously the Growing Point can be removed without killing it and that will also remove apical dominance from the remaining rhizome.

Here's a variegated banana that is being used for macropropagation. After the Growing Point was removed it was replanted to start process over again. This probably can go on for eternity using the same growing point.



__________________



PR-Giants Photo Gallery



15,000+ Varieties

PR-Giants is offline   Reply With Quote Send A Private Message To PR-Giants
Said thanks:
Old 08-30-2019, 08:19 AM   #25 (permalink)
 
mcfly's Avatar
 
Location: Northern California - East Bay
Zone: PST
Join Date: Aug 2018
Posts: 36
BananaBucks : 10,710
Feedback: 0 / 0%
Said "Thanks" 38 Times
Was Thanked 44 Times in 20 Posts
Said "Welcome to Bananas" 0 Times
Default Re: First Macropropagation experiments - Sddarkman619

Hi PR-Giants,

Thanks for your reply as well. I read that on top of removing/killing the main growth point, for maximum output, to identify growth points on the sides of the pared corn and slicing an ‘x’ on each one to obtain multiple growths out of each individual growth point. Not too deep as you don’t want to kill that growth point. However, I have not seen any close up pics to identify those growth points. It was referred to it as the PIF technique.

Then as the pups start shooting off, the bigger ones you are supposedly able to cut off (killing) and then you x in that growth point to get more pups from there. Like you mentioned, this process could go on for eternity.

Do you have a picture of your prepared corn, all pared down and ready to go? I am going to try with and expendable Basjoo I have in order to test the methods discussed.

Mcfly
__________________


Currently growing:
Basjoo
Blue Java "Ice Cream"

Recently started:
Black Thai
Saba
Mekong Giant
Ensete Glaucum “Snow Banana”
Variegated Florida - non AEAE
?Mystery banana?
Maybe Senorita
Maybe Namwa
Dwarf Brazillian
Red Zebrina
Ensete ventricosum 'Maurelii'
mcfly is offline   Reply With Quote Send A Private Message To mcfly
Old 08-30-2019, 10:11 AM   #26 (permalink)
Banana Man
 
sddarkman619's Avatar
 
Name: Larry
Join Date: Jul 2012
Posts: 1,148
BananaBucks : 188,606
Feedback: 14 / 100%
Said "Thanks" 340 Times
Was Thanked 1,219 Times in 535 Posts
Said "Welcome to Bananas" 1 Times
Default Re: First Macropropagation experiments - Sddarkman619

Watch this video:

Macropropagation from ProMusa Video Bank on Vimeo.

__________________
Ca Licensed Nursery - Snail/Disease Free and Inspected:
Bountiful Garden Nursery - A1469.001

Banana Plants for Sale
https://www.bananatreesforsale.com


Sign up to the Newsletter for Updates:
Sign Up Here
sddarkman619 is offline   Reply With Quote Send A Private Message To sddarkman619
Old 08-30-2019, 10:27 AM   #27 (permalink)
banana cereal killer
 
beam2050's Avatar
 
Location: middleburg fl.
Zone: 9b-8a
Name: walkinbeam
Join Date: Sep 2016
Posts: 2,423
BananaBucks : 572
Feedback: 0 / 0%
Said "Thanks" 10,695 Times
Was Thanked 3,349 Times in 1,543 Posts
Said "Welcome to Bananas" 816 Times
Default Re: First Macropropagation experiments - Sddarkman619

excellent video. makes you want to try it.
__________________
...................................................

npk of wood ash 0/1/3 to 0/3/7

npk of banana leaf ash 1.75/0.75/0.5
beam2050 is offline   Reply With Quote Send A Private Message To beam2050
Old 08-30-2019, 12:39 PM   #28 (permalink)
Commercial Grower
 
PR-Giants's Avatar
 
Location: Florida & Greater Antilles
Join Date: Feb 2012
Posts: 4,544
BananaBucks : 2,436
Feedback: 16 / 100%
Said "Thanks" 2,900 Times
Was Thanked 12,461 Times in 3,620 Posts
Said "Welcome to Bananas" 3,204 Times
Default Re: First Macropropagation experiments - Sddarkman619

There are many different techniques and understanding the basics will help in choosing what works best in each situation.

One plant can produce thousands of new plantlets but for us maximum output is less desirable than speed.

The variegated rhizome in the top photo will basically activate each those buds in a specific order and allow us to remove a large pup about every 6 days. This is the advantage of having it focus it's resources on just a few buds at a time.

The bottom photo is what happens by mutilating one of those buds.

My first post was to point out that the main plant can be removed and replanted instead of killing it.

I prefer to use clean coarse sand as the medium because it'll last forever, it drains well, has great aeration, and it's easy to keep the humidity perfect.

There are many options with macropropagation and understanding how apical dominance changes is interesting and useful.






Quote:
Originally Posted by mcfly View Post
Hi PR-Giants,

Thanks for your reply as well. I read that on top of removing/killing the main growth point, for maximum output, to identify growth points on the sides of the pared corn and slicing an ‘x’ on each one to obtain multiple growths out of each individual growth point. Not too deep as you don’t want to kill that growth point. However, I have not seen any close up pics to identify those growth points. It was referred to it as the PIF technique.

Then as the pups start shooting off, the bigger ones you are supposedly able to cut off (killing) and then you x in that growth point to get more pups from there. Like you mentioned, this process could go on for eternity.

Do you have a picture of your prepared corn, all pared down and ready to go? I am going to try with and expendable Basjoo I have in order to test the methods discussed.

Mcfly
__________________



PR-Giants Photo Gallery



15,000+ Varieties

PR-Giants is offline   Reply With Quote Send A Private Message To PR-Giants
Old 08-30-2019, 03:32 PM   #29 (permalink)
 
mcfly's Avatar
 
Location: Northern California - East Bay
Zone: PST
Join Date: Aug 2018
Posts: 36
BananaBucks : 10,710
Feedback: 0 / 0%
Said "Thanks" 38 Times
Was Thanked 44 Times in 20 Posts
Said "Welcome to Bananas" 0 Times
Default Re: First Macropropagation experiments - Sddarkman619

Quote:
Originally Posted by sddarkman619 View Post
Watch this video:

Macropropagation from ProMusa Video Bank on Vimeo.

Yes, this is one of the videos I saw. Great video. Great solution for that part of the world too. It really solved a problem.
__________________


Currently growing:
Basjoo
Blue Java "Ice Cream"

Recently started:
Black Thai
Saba
Mekong Giant
Ensete Glaucum “Snow Banana”
Variegated Florida - non AEAE
?Mystery banana?
Maybe Senorita
Maybe Namwa
Dwarf Brazillian
Red Zebrina
Ensete ventricosum 'Maurelii'
mcfly is offline   Reply With Quote Send A Private Message To mcfly
Old 08-30-2019, 03:48 PM   #30 (permalink)
 
mcfly's Avatar
 
Location: Northern California - East Bay
Zone: PST
Join Date: Aug 2018
Posts: 36
BananaBucks : 10,710
Feedback: 0 / 0%
Said "Thanks" 38 Times
Was Thanked 44 Times in 20 Posts
Said "Welcome to Bananas" 0 Times
Default Re: First Macropropagation experiments - Sddarkman619

Great info PR-Giants. Thanks for the pics too. I just need to get this started. Perhaps sometime during this three day weekend I'll have some time.

I don't have much in the way of a controlled environment ie no greenhouse but I have some areas that get less sun. A rubbermaid bin (or other) with some holes in the bottom, bedding cloth, then I'll try once with Coco coir and one with course sand. Place a plastic sheet on top and give it a run.


Quote:
Originally Posted by PR-Giants View Post
There are many different techniques and understanding the basics will help in choosing what works best in each situation.

One plant can produce thousands of new plantlets but for us maximum output is less desirable than speed.

The variegated rhizome in the top photo will basically activate each those buds in a specific order and allow us to remove a large pup about every 6 days. This is the advantage of having it focus it's resources on just a few buds at a time.

The bottom photo is what happens by mutilating one of those buds.

My first post was to point out that the main plant can be removed and replanted instead of killing it.

I prefer to use clean coarse sand as the medium because it'll last forever, it drains well, has great aeration, and it's easy to keep the humidity perfect.

There are many options with macropropagation and understanding how apical dominance changes is interesting and useful.




__________________


Currently growing:
Basjoo
Blue Java "Ice Cream"

Recently started:
Black Thai
Saba
Mekong Giant
Ensete Glaucum “Snow Banana”
Variegated Florida - non AEAE
?Mystery banana?
Maybe Senorita
Maybe Namwa
Dwarf Brazillian
Red Zebrina
Ensete ventricosum 'Maurelii'

Last edited by mcfly : 08-30-2019 at 04:57 PM.
mcfly is offline   Reply With Quote Send A Private Message To mcfly
Said thanks:
Old 02-13-2020, 06:01 PM   #31 (permalink)
 
Backyard Banana Joe's Avatar
 
Location: Orlando FL
Zone: 9B
Join Date: Jul 2019
Posts: 1,428
BananaBucks : 95,267
Feedback: 3 / 100%
Said "Thanks" 6,976 Times
Was Thanked 1,984 Times in 935 Posts
Said "Welcome to Bananas" 184 Times
Smile Re: First Macropropagation experiments - Sddarkman619

I think I will try this as well....... I will make a new thread of I do it
Backyard Banana Joe is offline   Reply With Quote Send A Private Message To Backyard Banana Joe
Said thanks:

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Reply   Email this Page Email this Page

Previous Thread: Malt Extract Agar?
Next Thread: Banana stump sprout.





Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Growing hormones, stimulators experiments. A lill bit of practical knowledge. tomekmwa Banana Plant Soil, Additives, and Fertilizer 10 08-22-2018 04:19 PM
Macropropagation for basjoo? H2O Tissue Culturing & Other Propagation Techniques of Banana Plants 3 05-21-2012 01:58 AM


All times are GMT -5. The time now is 01:44 AM.





All content © Bananas.org & the respective author.