Bananas.org

Welcome to the Bananas.org forums.

You're currently viewing our message boards as a guest which gives you limited access to participate in discussions and access our other features such as our wiki and photo gallery. By joining our community, you'll have access to post topics, communicate privately with other members (PM), respond to polls, upload photos, and access many other special features. Registration is fast and simple, so please join our community today!

If you have any problems with the registration process or your account login, please contact us.

Go Back   Bananas.org > Banana Forum > Tissue Culturing & Other Propagation Techniques of Banana Plants
The Facebook Platform
Register Photo Gallery Classifieds Wiki Chat Map Search Today's Posts Mark Forums Read

Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


Members currently in the chatroom: 0
The most chatters online in one day was 17, 09-06-2009.
No one is currently using the chat.

Reply   Email this Page Email this Page
 
LinkBack Thread Tools
Old 01-31-2009, 08:43 PM   #1 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default explant size?

What size plant can you start with for an explant for TC?

I have a few plants inside for the winter. My girlfriend noticed a sport popping up next to the main stalk...couple days later....another. So....I want to use one...or both...of these pups as explants for tc. The variety is Rajapuri.

http://www.bananas.org/gallery/uploa...plants_015.JPG

http://www.bananas.org/gallery/uploa...plants_013.JPG
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 01-31-2009, 10:34 PM   #2 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

Generally, I ask for them to be at least 16" tall, I have done smaller, but dividing the tissue becomes more difficult.



For reference, this is a baby food jar cap.
__________________
Scot


Click for Jakarta, Indonesia Forecast

Last edited by Chironex : 02-02-2009 at 12:57 PM.
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Said thanks:
Old 02-01-2009, 06:23 PM   #3 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

wow that large!

At the lab i work at it's all about the smallest piece possible. would it be possible to take a pic of a "starting plant" and how much you actually use?
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Old 02-01-2009, 09:22 PM   #4 (permalink)
Moderator

 
Gabe15's Avatar
 
Location: Oahu, Hawaii
Zone: 12
Name: Gabe
Join Date: Jul 2005
Posts: 3,531
BananaBucks : 313,884
Feedback: 5 / 100%
Said "Thanks" 1 Times
Was Thanked 6,699 Times in 1,815 Posts
Said "Welcome to Bananas" 8 Times
Default Re: explant size?

The bigger the plant, the easier it is to keep it sterile. With a large plant, you can do surface sterilization multiple times while removing layers from around the meristem, but with a plant as small as the ones you want to use, you would have to get it very sterile on the first try and would probably be able to only remove one layer.
__________________
Growing bananas in Colorado, Hawaii and Washington since 2004.
Gabe15 is offline   Reply With Quote Send A Private Message To Gabe15
Said thanks:
Old 02-01-2009, 10:18 PM   #5 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

Quote:
Originally Posted by MurCurY View Post
wow that large!

At the lab i work at it's all about the smallest piece possible. would it be possible to take a pic of a "starting plant" and how much you actually use?
There is a photo in my gallery.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Sponsors

Old 02-02-2009, 07:33 AM   #6 (permalink)
Bananaculturist
 
Bananaman88's Avatar
 
Location: Pearland, TX
Zone: 9
Name: Brent
Join Date: Jun 2007
Posts: 2,997
BananaBucks : 150,109
Feedback: 22 / 100%
Said "Thanks" 1,289 Times
Was Thanked 2,205 Times in 1,150 Posts
Said "Welcome to Bananas" 187 Times
Send a message via Skype™ to Bananaman88
Default Re: explant size?

MurCurY,

I'm just trying to make sure that I understand you. When you say "sport" do you mean an actual sport where what you see coming up is distinctively different from the parent plant or do you simply mean a "pup"? Thanks for the clarification.
Bananaman88 is offline   Reply With Quote Send A Private Message To Bananaman88
Old 02-02-2009, 06:27 PM   #7 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

i found the post with that pic chironex....but when i went to your gallery....couldn't find it.

I have a cavendish super dwarf that's at leaf 12/13 on 2 pups....the main plant died off a while ago. Maybe i'll sacrifice one of these plants and give it a try.
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Old 02-02-2009, 06:30 PM   #8 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

a pup....


and chironex...that tip in that baby food jar lid came from a 16" or so plant?

How hard is it to find? are there layers leading to the tip....like an onion?
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Old 02-02-2009, 10:22 PM   #9 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

Quote:
Originally Posted by MurCurY View Post
a pup....


and chironex...that tip in that baby food jar lid came from a 16" or so plant?

How hard is it to find? are there layers leading to the tip....like an onion?
It is similar to an onion, yes. The closer you get to this tissue, the more compact it becomes. Don't peel away too much before you sterilize the surface though. As Gabe has said, you will lose some of the outer layers of tissue in the process.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 02-03-2009, 06:54 PM   #10 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

and you use standard MS media......right?
any hormones?
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Old 02-03-2009, 08:06 PM   #11 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

As Gabe mentioned before, we generally use 1/2 strength M&S medium, with BA or BAP during initiation. I have been experimenting with other media too, including kinetin and activated charcoal. Although, these are primarily to promote embryo rescue.

Then no hormones for rooting, or some use NAA, IBA or IAA (auxins) but I haven't tried them yet, as all of my cultures are still in stage 2 sub-culturing.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 02-27-2009, 03:12 PM   #12 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

1/2 strength MS, with what concentrations of BAP and...do you not use NAA in the initial mix?
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Old 02-27-2009, 03:26 PM   #13 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

Quote:
Originally Posted by MurCurY View Post
1/2 strength MS, with what concentrations of BAP and...do you not use NAA in the initial mix?
Generally, 1 mg/ml is adequate although I have seen much lower concentrations of BAP. I believe that this will vary based upon which banana cultivar is being cultured.

I do not use NAA until the rooting phase as it is a rooting auxin. Some add no NAA and just leave out the plant growth regulators altogether in phase 3 - rooting. Try using some in a few cultures and none in others until you find which one works best for your banana plant selection.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 02-27-2009, 03:40 PM   #14 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

so....1mg per liter of agar? I'm using BAP dissolved in dmso.
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Old 02-27-2009, 03:49 PM   #15 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

No, 1 mg/ml in water. If you are dissolving it in DMSO use about 25 mg/ml. You will use about 5 mg of this BAP solution in your agar.
Note: It's quite alkaline, so be sure to adjust your pH.
This link might be of help to you: Micropropagation of Musa
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Said thanks:
Old 02-27-2009, 04:19 PM   #16 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

excellent site!

One last question....for the moment....
He lists gelrite as his "stiffener"
What amount of agar would you use for musa?
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY

Join Bananas.org Today!

Are you a banana plant enthusiast? Then we hope you will join the community. You will gain access to post, create threads, private message, upload images, join groups and more.

Bananas.org is owned and operated by fellow banana plant enthusiasts. We strive to offer a non-commercial community to learn and share information. Receive all three issues from Volume 1 of Bananas Magazine with your membership:
   

Join Bananas.org Today! - Click Here


Sponsors

Old 02-27-2009, 04:38 PM   #17 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

I use about 5-7 grams per vessel. I put about 3 tablespoons of medium per vessel and add 5-7 grams of agar. (This is about a third of a teaspoon of agar per vessel.) Gelrite is used instead of agar by some people. I like it more because it is colorless.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 03-01-2009, 08:39 AM   #18 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

i've been really sick past few days. i'm feeling way better today so....i think i'm going to make up my agar and start disinfecting this afternoon/evening.

5-7g of agar in approx. 40ml of nutrient.......isn't that rock hard? when i make agar for my mushrooms i use 20g/l and it's firm enough i can't press into it but my finger makes a print when pressed.

I already mixed my 6-BA at 1mg/ml in dmso and do not have an accurate enough scale at home to add up the mix...my work lets me use their lab scale and i've been out sick the past few days.
Would using 5ml of dmso in a liter of agar be harmful to the explant? The lab manager says she "thinks a little helps the plant take up stuff better"....although i don't really know. and i've never asked her if there's an amount at which it might be detrimental. I do know if you touch it...your mouth tastes kind of like garlic in seconds.

I have some strawberry plants from work i might try to tc while i'm at it.

Last edited by MurCurY : 03-01-2009 at 08:45 AM.
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Old 03-01-2009, 09:06 AM   #19 (permalink)
The causasian Asian!
 
Chironex's Avatar
 
Location: Jakarta, Indonesia
Zone: I have no idea
Name: Scot
Join Date: Apr 2008
Posts: 2,788
BananaBucks : 60,777
Feedback: 9 / 100%
Said "Thanks" 4,544 Times
Was Thanked 1,375 Times in 793 Posts
Said "Welcome to Bananas" 609 Times
Send a message via MSN to Chironex Send a message via Yahoo to Chironex
Default Re: explant size?

You can use less agar if you wish, I was going from memory and I use a pre-sized measuring spoon when I use agar. I use 7 g of gelrite.
Not sure about DMSO as I have not used it. Good luck.
__________________
Scot


Click for Jakarta, Indonesia Forecast
Chironex is offline   Reply With Quote Send A Private Message To Chironex
Old 03-01-2009, 09:50 AM   #20 (permalink)
 
Join Date: Jun 2008
Posts: 13
BananaBucks : 5,857
Feedback: 0 / 0%
Said "Thanks" 0 Times
Was Thanked 23 Times in 3 Posts
Said "Welcome to Bananas" 0 Times
Default Re: explant size?

thank you very much! pictures to follow.
MurCurY is offline   Reply With Quote Send A Private Message To MurCurY
Sponsors

Reply   Email this Page Email this Page

Previous Thread: Getting started soon
Next Thread: Re: Tissue Culture





Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)
 
Thread Tools



All times are GMT -5. The time now is 04:13 AM.





Follow us:
Twitter YouTube

All content © Bananas.org & the respective author.