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Tissue Culturing Banana Plants This forum is for discussing tissue culturing of banana plants. Tissue culturing is the process of creating clones from a source plant.

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Old 04-11-2007, 06:13 PM   #1 (permalink)
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Default Ensete Maurelii propagation?

I read the best way to do this (apart from tissue culture) is at the peak of growth (mid July maybe) to chop the top off close to the ground, cover it with sand and wait nervously for pups.

Has anyone actually tried it? or have any suggestions? Mine's gonna be big this year and I'd like to get a few pups on the go if poss.
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Old 04-11-2007, 08:28 PM   #2 (permalink)
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Default Re: Ensete Maurelii propagation?

Yes I have...It all started after a week of -7c in a pot.My basjoo and maurelii's trunks turned to mush, so I brought them inside and cut the trunks down to 8-10 inches...about two weeks later they began to push out the old frozen leaf.In January I decided to try propagatin the Maurelii so I cut it down to 2 inches above the soil.Cut it down the center and cut a v in it.I have pictures in my gallery.
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Old 04-12-2007, 12:48 AM   #3 (permalink)
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Default Re: Ensete Maurelii propagation?

..and here are a few of those photos:









More info about this banana is in the wiki
http://www.bananas.org/wiki/Ensete_ventricosum_'Maurelii'
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Old 04-12-2007, 08:10 AM   #4 (permalink)
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Default Re: Ensete Maurelii propagation?

"V" for victory then!

Thanks for litterarly taking the sword in your hands and trying this theory out! I have allways been too chicken to try this with my ensetes, but this fall I will put one "under the knife"!

Erlend
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Old 04-12-2007, 08:40 AM   #5 (permalink)
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Default Re: Ensete Maurelii propagation?

Hi Guys here is another old post from me with pics of maurelii pups. What happens in Europe often is a maurelii will get the straggles and we cut the top down to try and free up the leaf growth again, this works about half the time for me. If cutting does not work then often the main stem is lost and what you get is a load of pups if you wait long enough. Anyway here is the old thread with pics of pups.
Ensete Ventricosum Maurelii follow up pics

Later
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Old 04-12-2007, 07:54 PM   #6 (permalink)
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Default Re: Ensete Maurelii propagation?

That is really neat! These may be dumb questions but, how do you seperate all the pups? Do you need to wait until they're larger or do you take a knife to them when young? Does this work with musa?
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Old 04-12-2007, 08:10 PM   #7 (permalink)
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Default Re: Ensete Maurelii propagation?

Also I thought pups must have roots in order to survive.
Must you dig down deep and separate the main corm or devide the main corm?
As I am new to this.
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Old 04-14-2007, 02:05 AM   #8 (permalink)
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Default Re: Ensete Maurelii propagation?

I am also new to this....I was hoping someone would have information on this aswell, as I am going to attemp this part in the next week. I saw as the "callus"[ which at first I thought was a mushroom] ,was growing up from the left side of the V , had bumps-warty growths. From these ,out came the "pups" .On some of these pups you could see small roots running between these bumps, so I put potting mix onto the whole plant.Now because I did that ,dividing the pups is going to be a real chore...I might rince the dirt off before I divide them...any ideas?
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Old 04-14-2007, 02:15 AM   #9 (permalink)
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Default Re: Ensete Maurelii propagation?

My advice would be to let them grow to about 1-2ft, then dig up the mass and quarter it and replant each section. Then continue that process as they grow and you should end up with many plants when you narrow it down to the nice vigorous shoots.
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Old 04-14-2007, 04:07 AM   #10 (permalink)
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Hiya Re: Ensete Maurelii propagation?

I saw a programme on TV a while back showing commercial Tulip culture in Holland. what they were doing was to get a bulb, quarter it or chop it into eighths vertically like you would a tomato. the 'segments' were then put on damp vermiculite in a warm dark propagation room. A few weeks later roots and sprouts and away you go.
It might work just the same with the Ensete. A good way to go from 1 to 8. the 'key' was to have the tiniest bit of rooting tissue on each segment
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Old 04-15-2007, 01:19 PM   #11 (permalink)
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Default Re: Ensete Maurelii propagation?

So has anyone tried this with a musa variety. It seems like it'd work just the same, but before I go all lumber jack...
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Old 06-22-2007, 01:33 PM   #12 (permalink)
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Default Re: Ensete Maurelii propagation?

Just curious - should the "stress" be induced before the plant flowers - or does it matter?
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Old 06-23-2007, 12:27 AM   #13 (permalink)
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Default Re: Ensete Maurelii propagation?

Quote:
Originally Posted by D' Andra View Post
So has anyone tried this with a musa variety. It seems like it'd work just the same, but before I go all lumber jack...
http://www.ceniap.gov.ve/ceniaphoy/a.../gmartinez.htm
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Old 06-23-2007, 02:28 AM   #14 (permalink)
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Default Re: Ensete Maurelii propagation?

Oye! No hace falta buscar Mas. Muchas Gracias Lagniappe!

I hope the above gets everyone ready to read that link. Really cool info in it though.

That does it! I'm taking the axe to a couple of plants.
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Old 06-23-2007, 02:54 AM   #15 (permalink)
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Default Re: Ensete Maurelii propagation?

Quote:
Originally Posted by AS in SA View Post
Just curious - should the "stress" be induced before the plant flowers - or does it matter?
It needs to be before the plant has flowered, really before it has even begun to make to make the flower because the whole point of it is to damage the meristem but if it is flowering then the meristem would have already moved on and is in the infloresence.
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Old 06-23-2007, 09:13 AM   #16 (permalink)
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Default Re: Ensete Maurelii propagation?

Very nice link indeed!:2190:
Thank you so much, Lagniappe!
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Old 06-23-2007, 10:03 AM   #17 (permalink)
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Default Re: Ensete Maurelii propagation?

YVW ! I tried to post this site with google translater already in the link ....Guess it was too advanced for me .
You guys that understand the flux capacitors and such should post it in a seperate thread .
Pete


http://64.233.179.104/translate_c?hl.../gmartinez.htm
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Last edited by Lagniappe : 06-23-2007 at 10:09 AM.
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Old 06-23-2007, 03:01 PM   #18 (permalink)
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Default Re: Ensete Maurelii propagation?

Great Scott!

http://babelfish.altavista.com/babel...Fgmartinez.htm

Digital magazine of the National Center of Farming Investigations of Venezuela

CENIAP TODAY
Not 4. January-April 2004
Technical manual for the Propagation of Musáceas
Gustavo Martinez*, Omar Tremont ** and Julitt Hernandez ***
* CENIAP/INIA, Maracay
** UNEFM, Faculty of Agronomy, Choir
*** INIA/CIAE Yaracuy, San Felipe
gmartinez@inia.gov.ve
Introduction The cultures of banana, banana tree and topocho, pertaining to the family of the musáceas, have significant an economic importance for the agro-alimentary economy of the country, and constitute a basic component in the diet of great part of the population. They have the singular and individual incapacity to produce viable seeds and single is possible the reproduction and perpetuación of the species through the vegetative or asexual propagation (agámicas plants).
Therefore, the "used seeds" for seedtime correspond to vegetative parts: sprouts, cormos or children who, once separated of the plant mother, can make their cycle of growth and production.
The selection of the propagation material is the first step to initiate commercial seedtime of the culture, and most of the producers uses "originating seeds" of deshije (necessary basic work and in these cultures) reason why does not represent a significant increase in the production costs and being considered as the plus I practice and simple at field level.
Nevertheless, it exists a high probability of dissemination of plagues or other harmful agents, within the plantation when not existing the cares and necessary fitosanitarias precautions (Nava, 1980; Tezenas, 1985; Sandoval ET to., 1991) between which they are possible to be mentioned the complex of nematodes (Radopholus similis, Helycotlenchus, Pratylenchus spp.), the weevil or picudo (Cosmopolites sordidus), the Marasmiellus fungi troyanus and Fusarium oxysporum sp. Cubense, bactaerias caratovora Erwinia and Pseudomonas solanacearum (Fenjves; Nava and Sosa; Ordosgoytty ET to.; Yépez ET to., mentioned by Nava and Villarreal, 1980) and, finally, seeds of weeds, between which are species like corocillo (Cyperus diffusus), straw pelua (exaltata Rotiobollia), false Jonson (halepense Sorghum), gamelote (Panicum maximun) (Navas and Villarreal, 1980).
All of them can go with the seed, mainly in the conditions of the zone of production of the South of the Lake of Maracaibo, where the agriculturist seeds seeds without selection nor treatment of no type, which facilitates the dispersion of such harmful agents in the new plantations (Nava and Villarreal, 1980).
Most recommendable it is than the agriculturist selects the sowing material or "seed" from plants vigorous mothers, without visual signs of attacks of plagues and diseases, making cleaning and disinfection of same the selected children must be type sword, avoiding the use of those catalogued like orejones or water, which had that are lost their vitality for different reasons like desbalance nutricional, hydric stress, among others.
Similarly, it is possible to emphasize that generally these "seeds" present/display sizes and variable ages, that they originate different plants with vigor, causing differences marked at the time of maturation and harvests. It implies the necessity to use greater amount of wages and insumos, as well as adjustments in the activities related to the commercialization of the product.
One of the main factors of success in the commercial operation of these cultures is the obtaining and availability of "seeds" or material of propagation in sufficient amount and adapted quality, without it implies an increase disturbed in the costs; and less important it is not the establishment in field of homogenous plantations, with vigorous growth, that facilitate the workings of maintenance and uniform fruition.
The availability of a material of sowing in optimal conditions (desirable appearance, excellent fitosanitario aspect, among others) that facilitates the installation of production systems with uniform plants in its rate of physiological development, and that allows to make workings of harvest of the most efficient way, single has been possible through the use of vitroplantas (originating of biotechnologies as the weave culture in vitro), but to a relatively high cost, limiting the small and medium producer for its acquisition.
However, through an exhaustive selection of plants in the breeding ground stage, put under a strict control, also it has been possible to obtain this objective; for as the application of fitosanitarias measures is necessary, together with the practices normally used in the technified handling of the plantation, where those can be considered applied to the seeds: bare, chemical and physical treatments (heat), establishment of rank of size (small, medium, great) among others (Nava, 1980; Nava ET to., 1980).
Diverse methods and forms of propagation exist, with different variants (Adelaja, 1995; Alves and Oliveira, 1993; Belalcazar, 1991; Bonte ET to., 1995; Haddad ET to., 1994), but no of these systems of propagation, including those related to the biotechnology, totally guarantees the production of free materials of some virus and bacteria.
The objective of this article is to disclose the conceptual bases of the propagation of musáceas and to give to know the foundations the used techniques more, as well as to characterize some alternatives that facilitate this work. It is indicated in addition, the use of organic installments like tool before seedtime in field, with the purpose of obtaining vigorous plants, of so large uniform and excellent quality, increasing the possibilities of managing the establishment of homogenous plantations and consequently to improve the yields. The information is based on obtained results in tests of field, the experiences of the producers and an extensive bibliographical revision.
Morphology of the musáceas
From the taxonomic point of view the bananas, banana trees and topochos are located within the botanical family of the Musáceas, I generate Musa, considered like perennial estolonÃ*feras grass, (Belalcazar, 1991; Simmonds, 1973); with absence of viable seeds in most of the cases, that they allow his sexual propagation. Due to this, its reproduction is strictly vegetative, through the use of children or sprouts; which implies that the obtaining of "seed" of quality is difficult and requires of greater time and effort.
The musáceas are characterized to present/display two important stages during their development:
1) Stage of vegetative development: where the true stem is short and underground, with extremely short entrenudos, and small knots in the interception of the bases of the pecÃ*olos of the leaves, where the vegetative yolks are located that will give to origin to the sprouts or children. The rate of development of these is affected by the existing apical dominancia in the plant mother.
2) Stage of floral differentiation and production: where the vegetative development stops, with evident culmination of the emission to foliar, and physiological changes are originated that allow the rupture of the apical dominancia. The formation of floral structures begins and consequent formation of the cluster, with emergency or elongación of the underground stem that it will become aerial stem. In this stage greater development of the children or sprouts is observed.
Figure 1. Cross section of cormos of musáceas
In both stages one defines the origin and conformation of the children, who could be used as replacement of the plant mother or like new units of production. In this sense, to include/understand better the principle of the propagation techniques it is necessary to know the basic structure cormo.
Figure 1 presents/displays the longitudinal section of cormo of banana, being appraised in the superior part how the bases of the cases of the leaves are arranged one on another one, forming a vault that covers and protects the central or apical yolk (meristemo), person in charge of the vegetative and productive activity of the plant mother.
In the interception point of each base of the cases, located in the knot, the lateral yolk is originated that can be physiologically active or latent, and its development will allow the emission of the children. The number of these yolks and the physiological state that present/display (activate or latent), will depend on the type of clone to use (banana, banana tree or topocho), size and age of cormo, among others.
Its development is controlled by a set of physiological processes, that it possibly includes regulating of the growth, able to inhibit the activation, development and growth of these yolks, while the apical yolk is in active growth, which have been denominated "effect of apical dominancia", and stops at the time of taking place the harvest (Belalcazar, 1991), or when the apical yolk is destroyed by the man or natural phenomena (floods, droughts), among others.
Systems of propagation
With base in the previous considerations, it is evident that the commercial propagation of the musáceas obeys only to asexuales methods, existing systems or techniques that vary essentially in agreement with the type and disposition of infrastructure, costs and necessary technical qualification. Next a description of the used systems will be made more of simple way, taking in consideration that even though the mentioned technique is based on very simple foundations, will have to be taken into account the elementary norms from asepsis for its execution, with the purpose of as far as possible avoiding the contamination of other materials and new areas of sowing. In order to facilitate the compression and applicability of the systems, in Picture 1 a comparative summary of such appears, emphasizing the most important characteristics of each one.
Picture 1. - Qualitative characteristics of the different methods from propagation Characteristics
Sistema
Traditional Division cormos Division I appear