Can someone post a tc guide?

imclueless17 · 07-09-2010, 05:30 PM

Hi I really want to get into tissue culturing bananas. So my question is can someone post a complete guide to tissue culturing bananas in particular, Including at least some the following: materials needed, what media (or media's) to use to properly grow bananas, how to do all of the work including cutting down the meristem, and how to keep everything sterile. Big thanks to all u guys on the org!

Abnshrek · 07-09-2010, 05:45 PM

Info:Tissue Culturing - Bananas Wiki :^) Search the T/C threads as well or contact Novi.. via pm.. :^)

Gabe15 · 07-10-2010, 12:44 AM

Tissue culture is a rather complex process. It's not difficult if you know what you are doing, but to explain every detail in writing would take a very long time, and still be very confusing. More importantly, tissue culture is not unique to bananas at all, so any guides you find about tissue culturing bananas will be more on the specifics of banana in tissue culture, but it will not explain all of the really basic tissue culture things. I recommend you do some research on tissue culture in general, do not even think about bananas at first, and once you understand what it involves and get an idea of how it works, then focus on what is needed specifically for bananas.

My personal feeling is that unless you need to make over 1000 plants, tissue culture is not the best option for the hobbyist looking to propagate. It's fun and interesting and has many non-propagation uses, but if you are just looking to multiply the plants you have, it can be expensive, time consuming, confusing and might not meet your needs.

I have a great tissue culture alternative which I plan to share with the group soon. I am making a fully illustrated guide on techniques that can allow the hobbyist to produce many (not as much as tissue culture but more than enough for the average hobbyist) cloned banana plants in a much cheaper, quicker and easier fashion than with tissue culture.

chasbear · 07-10-2010, 04:09 AM

Quote:

Originally Posted by Gabe15

I have a great tissue culture alternative which I plan to share with the group soon. I am making a fully illustrated guide on techniques that can allow the hobbyist to produce many (not as much as tissue culture but more than enough for the average hobbyist) cloned banana plants in a much cheaper, quicker and easier fashion than with tissue culture.

I for one will be anxiously awaiting your guide, as I have also toyed with the idea of starting my own tissue culture lab for my expansion here. Yes, I could use more than 1000 plants at a time, but this also has its own hassles. However, if its "cheaper, quicker and easier," then I'm in!

jmoore · 07-10-2010, 07:22 AM

Wow Gabe I'm greatly interested in that.

oakshadows · 07-31-2011, 07:01 PM

Quote:

Originally Posted by Gabe15

Tissue culture is a rather complex process. It's not difficult if you know what you are doing, but to explain every detail in writing would take a very long time, and still be very confusing. More importantly, tissue culture is not unique to bananas at all, so any guides you find about tissue culturing bananas will be more on the specifics of banana in tissue culture, but it will not explain all of the really basic tissue culture things. I recommend you do some research on tissue culture in general, do not even think about bananas at first, and once you understand what it involves and get an idea of how it works, then focus on what is needed specifically for bananas.

My personal feeling is that unless you need to make over 1000 plants, tissue culture is not the best option for the hobbyist looking to propagate. It's fun and interesting and has many non-propagation uses, but if you are just looking to multiply the plants you have, it can be expensive, time consuming, confusing and might not meet your needs.

I have a great tissue culture alternative which I plan to share with the group soon. I am making a fully illustrated guide on techniques that can allow the hobbyist to produce many (not as much as tissue culture but more than enough for the average hobbyist) cloned banana plants in a much cheaper, quicker and easier fashion than with tissue culture.

Very interestng, did it happen yet ? Following most of your posts I think this will be one to surely want to see.
Thanks

Gabe15 · 07-31-2011, 09:59 PM

I'm still working on it. The problem is I had many photos of the process, but due to some unfortunate hard drive accidents I lost many of them. I will be working on it more in the next few months as I will be using the technique for my masters project.

funboy30189 · 07-12-2012, 07:35 PM

gabe any more progress?

07-09-2010, 05:30 PM	#1 (permalink)
imclueless17 Never Look Down... imclueless17 's Photo Gallery Zone: 6-7 Name: Jamie Join Date: Apr 2010 Posts: 138 BananaBucks : 83,763 Feedback: 1 / 100% Said "Thanks" 262 Times Was Thanked 59 Times in 47 Posts Said "Welcome to Bananas" 8 Times	Can someone post a tc guide? Hi I really want to get into tissue culturing bananas. So my question is can someone post a complete guide to tissue culturing bananas in particular, Including at least some the following: materials needed, what media (or media's) to use to properly grow bananas, how to do all of the work including cutting down the meristem, and how to keep everything sterile. Big thanks to all u guys on the org! __________________ E Pluribus Unum

07-09-2010, 05:45 PM	#2 (permalink)
Abnshrek Happy Growing Abnshrek 's Photo Gallery Location: Beaumont Texas Find me on the map! Zone: 8b, but 9b weather.. Name: Migael / Michael Join Date: Aug 2009 Posts: 9,492 BananaBucks : 196,486 Feedback: 45 / 100% Said "Thanks" 10,447 Times Was Thanked 16,440 Times in 5,237 Posts Said "Welcome to Bananas" 2,318 Times	Re: Can someone post a tc guide? Info:Tissue Culturing - Bananas Wiki :^) Search the T/C threads as well or contact Novi.. via pm.. :^) __________________ I'm a Nannerhead :^)

07-10-2010, 12:44 AM	#3 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,892 BananaBucks : 13,339,501 Feedback: 5 / 100% Said "Thanks" 1 Times Was Thanked 8,237 Times in 2,200 Posts Said "Welcome to Bananas" 8 Times	Re: Can someone post a tc guide? Tissue culture is a rather complex process. It's not difficult if you know what you are doing, but to explain every detail in writing would take a very long time, and still be very confusing. More importantly, tissue culture is not unique to bananas at all, so any guides you find about tissue culturing bananas will be more on the specifics of banana in tissue culture, but it will not explain all of the really basic tissue culture things. I recommend you do some research on tissue culture in general, do not even think about bananas at first, and once you understand what it involves and get an idea of how it works, then focus on what is needed specifically for bananas. My personal feeling is that unless you need to make over 1000 plants, tissue culture is not the best option for the hobbyist looking to propagate. It's fun and interesting and has many non-propagation uses, but if you are just looking to multiply the plants you have, it can be expensive, time consuming, confusing and might not meet your needs. I have a great tissue culture alternative which I plan to share with the group soon. I am making a fully illustrated guide on techniques that can allow the hobbyist to produce many (not as much as tissue culture but more than enough for the average hobbyist) cloned banana plants in a much cheaper, quicker and easier fashion than with tissue culture. __________________ Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties. Last edited by Gabe15 : 07-10-2010 at 04:54 AM.

07-10-2010, 07:22 AM	#5 (permalink)
jmoore jmoore 's Photo Gallery Location: Lowestoft, UK Zone: 8a Name: James Join Date: Aug 2008 Posts: 766 BananaBucks : 118,897 Feedback: 4 / 100% Said "Thanks" 192 Times Was Thanked 799 Times in 373 Posts Said "Welcome to Bananas" 71 Times	Re: Can someone post a tc guide? Wow Gabe I'm greatly interested in that.

07-31-2011, 09:59 PM	#7 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,892 BananaBucks : 13,339,501 Feedback: 5 / 100% Said "Thanks" 1 Times Was Thanked 8,237 Times in 2,200 Posts Said "Welcome to Bananas" 8 Times	Re: Can someone post a tc guide? I'm still working on it. The problem is I had many photos of the process, but due to some unfortunate hard drive accidents I lost many of them. I will be working on it more in the next few months as I will be using the technique for my masters project. __________________ Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.

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07-12-2012, 07:35 PM	#8 (permalink)
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