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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 06-19-2008, 11:41 AM   #1 (permalink)
 
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Default Banana Tissue Culture

Hello all

I joined the Banana.Org Forum primarily for information on Bananas - growing bananas for a living. I have read a vast amount of information that is available on the web and would like to start my retirement enterprise from a scratch - that is from seed to a banana plantation.

I have been taken in by propagation of bananas by way of tissue culture and would like to try my hand at it but the laboratory-type regime does not suit the environment I am going to be operating in.

Firstly, at present there is no electricity but have a small generator and solar power to charge batteries, lights, etc.

I would like to hear from members who have had experience with tissue culture to help with information and suggestions on how this can be done in a rural environment in Zambia devoid of mod-cons.

I can set up a workable rudimentary laboratory to do the work required and get the hands-on experience to see if tissue culture would be a viable solution to propagating bananas that are disease-free.

A lot of work is being done in East Africa, West Africa but nothing is being done in Zambia although some commercial enterprises have been talking about it but is not viable proposition as yet.

Help will be much appreciated.
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Old 06-19-2008, 12:51 PM   #2 (permalink)
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Default Re: Banana Tissue Culture

Hey! A Big Welcome from Texas!!! It looks like you some very interesting work ahead of you!! I hope you will keep us updated on your progress!!! I can't answer your questions BUT I do look forward to seeing you around!!
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Old 06-19-2008, 12:55 PM   #3 (permalink)
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welcome! you have got a long road and a lot of work ahead of you. glad you joined us here at the org!!!
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Old 06-19-2008, 06:57 PM   #4 (permalink)
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Default Re: Banana Tissue Culture

Good luck with your plans. There are several threads here for tissue culture and I am sure you will find abundant information on these pages. Everyone here is eager to help you learn.
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Old 06-19-2008, 07:12 PM   #5 (permalink)
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Default Re: Banana Tissue Culture

welcome to Bananas.org. Gabe is are most educated member. hes talked at length regarding this subject. if you do a such I'm sure you will find at least something to get you started there are some vary good books on this topic but again Gabe would probably know better then i which periodicals and papers to direct you to.
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Old 06-19-2008, 09:44 PM   #6 (permalink)
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Gabe is are most educated member.
amen to that!!!!!
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Old 06-20-2008, 07:18 PM   #7 (permalink)
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By the way ...

Quote:
Originally Posted by malikopo39 View Post
I joined the Banana.Org Forum primarily for information on Bananas - growing bananas for a living. I have read a vast amount of information that is available on the web and would like to start my retirement enterprise from a scratch - that is from seed to a banana plantation.
edible seedless bananas cannot be grown from seed ... they are seedless.
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Old 10-30-2008, 09:45 PM   #8 (permalink)
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Default Re: Banana Tissue Culture

Quote:
Originally Posted by malikopo39 View Post
Hello all

I joined the Banana.Org Forum primarily for information on Bananas - growing bananas for a living. I have read a vast amount of information that is available on the web and would like to start my retirement enterprise from a scratch - that is from seed to a banana plantation.

I have been taken in by propagation of bananas by way of tissue culture and would like to try my hand at it but the laboratory-type regime does not suit the environment I am going to be operating in.

Firstly, at present there is no electricity but have a small generator and solar power to charge batteries, lights, etc.

I would like to hear from members who have had experience with tissue culture to help with information and suggestions on how this can be done in a rural environment in Zambia devoid of mod-cons.

I can set up a workable rudimentary laboratory to do the work required and get the hands-on experience to see if tissue culture would be a viable solution to propagating bananas that are disease-free.

A lot of work is being done in East Africa, West Africa but nothing is being done in Zambia although some commercial enterprises have been talking about it but is not viable proposition as yet.

Help will be much appreciated.
You really don't need an elaborate laboratory. Tissue culture can be done in a cardboard box lined with foil or plastic. The main thing that you will need to do is to sterilize the equipment. 10-25% bleach and some 70% alcohol will help, but you will need a pressure cooker of some kind to be able to properly sterilize the jars, knives and most importantly the medium.
A small microwave oven could be used if you can get one. Boiling water won't kill all of the possible contaminants, so rely heavily on bleach water and alcohol.
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Old 10-31-2008, 01:09 AM   #9 (permalink)
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Welcome malikopo39, I sure admire your grit in your project. All the best!
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Old 10-31-2008, 01:41 AM   #10 (permalink)
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...................................Boiling water won't kill all of the possible contaminants, so rely heavily on bleach water and alcohol.
Scot
I believe you. A lot of nurses and doctors use, and highly recommend alcohol for sterilization. So, I know that my insides are fairly sterile, what with the all the single malt scotch that I consume. Heavy, you say?? Don't know about bleach though, I don't use it myself. (LOL, I couldn't help this one!)
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