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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 11-17-2014, 01:05 PM   #1 (permalink)
 
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Default ascorbic acid /lemon juice in xplant final rinse

I noticed rapid browning on the tissue after the final rinse while sectioning the xplant material prior to placing it on the media so on my last attempt at tissue culture # 1 was a failure 2 was some what of a success and 3 is looking great ) I decide to add a small amount of lemon juice to the rinse before sterilizing the water I am sure asorbic acid is what is doing the job but I did,nt have any on hand so lemon-juice was what I used rinsed half of the material in plain sterile water and half in sterile water with lemon juice the lemon treated xplants look much nicer with almost no discoloring and after a week are beginning to show green and starting to grow I would post some pics but saw that attaching photos to the post directly isn't recommended when I get time I will upload some to my gallery if any one tries this let me know how it works for you
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