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Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. Tissue culturing is the popular process of creating clones from a source plant. There are other techniques to propagate banana plants however, such as nicking corms or dividing corms. Learn more inside.


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Old 09-23-2009, 09:56 PM   #1 (permalink)
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Party 100 Bottle variegated bananas

Dear all
see 100 bottle variegated bananas in my gallery

(just comeback from hometown at west java to celebrate great day Idul Fitri 1430 H. happy Idul Fitri for all Moeslim all over the world that celebrate it. and in the way back I have seen a group black banana from high way. but i can not stop to take some photos)

regards

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Old 09-23-2009, 10:07 PM   #2 (permalink)
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Default Re: 100 Bottle variegated bananas

Nice work! And happy holiday(s) ...

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Old 09-24-2009, 01:57 AM   #3 (permalink)
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Default Re: 100 Bottle variegated bananas

Do you have a photo of a full grown plant? What is the name for this variety? Can you send me a or a friend one of these plants???
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Old 09-24-2009, 06:36 AM   #4 (permalink)
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Default Re: 100 Bottle variegated bananas

Dear Harvey, you can see in my gallery. there is few photos of variegated bananas
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Old 09-24-2009, 06:47 AM   #5 (permalink)
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Default Re: 100 Bottle variegated bananas

looking good keep up the hard work!
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Old 09-24-2009, 08:06 AM   #6 (permalink)
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Default Re: 100 Bottle variegated bananas

Hi Novi!!!
I am so excited for you & your accomplishments!
Was the Mother Explant an Ae Ae?
I have been reading where it is near impossible to TC...you have accomplished great things!!

Wonderful!! ~Cheryl
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Old 09-24-2009, 09:41 AM   #7 (permalink)
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Default Re: 100 Bottle variegated bananas

Hi Cheryl
the mother explant is Pisang Raja. I think Ae ae also can be propagated by TC. The problem is I have not yet Ae ae. if I can get one Ae ae, i can TC becuse almost every kind musa can be propagated by TC. I want to buy ae ae. does anyone sale for me? I also interested with musa velutina. help me...
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Old 09-24-2009, 10:37 AM   #8 (permalink)
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Default Re: 100 Bottle variegated bananas

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Originally Posted by novisyatria View Post
Dear Harvey, you can see in my gallery. there is few photos of variegated bananas
Thanks, but I don't see photos of any large variegated musa in your gallery, just the black.

My Ae Ae pups are too small at this point to remove to send you one and I'm afraid they may succumb to the cold winter weather in a few months. I'd love to see you give TC of Ae Ae a try, though.
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Old 09-24-2009, 12:55 PM   #9 (permalink)
 
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Default Re: 100 Bottle variegated bananas

Quote:
Originally Posted by novisyatria View Post
Hi Cheryl
the mother explant is Pisang Raja. I think Ae ae also can be propagated by TC. The problem is I have not yet Ae ae. if I can get one Ae ae, i can TC becuse almost every kind musa can be propagated by TC. I want to buy ae ae. does anyone sale for me? I also interested with musa velutina. help me...
I can help you out with some seeds or a plant of velutina. The plant is about 30cms high so still quite small. Not sure if it will make it to Indonesia.

Do you use your organic medium when propagating these variegated bananas? I'm really impressed with your results.
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Old 09-24-2009, 07:07 PM   #10 (permalink)
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Default Re: 100 Bottle variegated bananas

dear harvey
for TC, we dont need big plant. its better if smaal plant. bananas seedling heigt 30 cm/ just appearr from ground is good. even this plant have not yet leaf.
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Old 09-24-2009, 10:00 PM   #11 (permalink)
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Default Re: 100 Bottle variegated bananas

My biggest is about 30cm but I'm hoping for it to get big enough to remove and pot up to save it in my greenhouse as a back-up in case we have a cold winter. The others are very tiny and hardly moving, not really sure why....
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Old 09-24-2009, 10:17 PM   #12 (permalink)
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Default Re: 100 Bottle variegated bananas

Dear James
for Tc variegated bananas I use real stuff medium MS. organic mediumused for other kind bananas.
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Old 09-25-2009, 01:04 AM   #13 (permalink)
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Default Re: 100 Bottle variegated bananas

PM replied.
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Old 11-21-2009, 09:24 PM   #14 (permalink)
 
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Default Re: 100 Bottle variegated bananas

Quote:
Originally Posted by novisyatria View Post
Dear all
see 100 bottle variegated bananas in my gallery

(just comeback from hometown at west java to celebrate great day Idul Fitri 1430 H. happy Idul Fitri for all Moeslim all over the world that celebrate it. and in the way back I have seen a group black banana from high way. but i can not stop to take some photos)

regards

novi Syatria
You are great, Novi. Each time I visit these forums I get a bit more convinced that there are still nice people around. You are very helpful.
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Old 11-21-2009, 11:11 PM   #15 (permalink)
 
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You are great, Novi. Each time I visit these forums I get a bit more convinced that there are still nice people around. You are very helpful.
I forgot to tell you; you make me feel a bit sorry that Im not moeslem.
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Old 11-22-2009, 07:12 PM   #16 (permalink)
 
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Default Re: 100 Bottle variegated bananas

Great job novisyatria!

Do you have problems with phenolic extrudes? If so, how do you deal with it?
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Old 11-22-2009, 07:23 PM   #17 (permalink)
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Default Re: 100 Bottle variegated bananas

for variegated bananas and generally, there is no phenolic problem. phenolic compounds i see for black banana. the media become black. what i do is do subculture soon. if normally subculture 1 month after initiation, i do it 2 weeks after initiation. before the media become black all and its can be poison for explant.
the second problem solved, i try add active carcoal 0,2 gr/liter media. and so far the result is good. phenolic can be reduced.
hope can help all you with this information

regards
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Old 11-22-2009, 07:57 PM   #18 (permalink)
 
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Default Re: 100 Bottle variegated bananas

Thanks Novi!

I have a phenolic problem with Musa Basjoo. I used ascorbic acid (vitamin C) and that helped a little but not enough. Haven't tried activated charcoal yet.

Phenolic extrudes only happen about the third multiplication with these. The first two times it's not much of a problem. I'll have to start tranferring to new media every couple of weeks. Oh what fun that will be:-)

I love your setup. The compact florescent lights look nice and neat. You sure pack a lot of jars in there. Impressive!
May I ask what gelling agent you are using? Looks nice and clear.

Thanks again Novi!
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Old 11-22-2009, 08:12 PM   #19 (permalink)
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Default Re: 100 Bottle variegated bananas

I am using agar-agar that we can eat and can buy it in market. no special agar. this kind agar is cheap and easy to buy. if we can do TC with something
cheap and easy, so there is no reason why have to use expensive one.
in My home lab i use second hand jars, second hand culture rack, etc. even not all material is second hand. but i can reduced cost of TC.
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