My home tissue culture progress

Gabe15 · 06-25-2008, 12:46 AM

These are some photos of my first home grown tissue cultures, which have been growing for 6 days. You can see they have turned green and are starting to grow tiny leaves. When they grow large enough and start to form real leaves, pseudostems and pups (in a few more weeks), I can separate them and put them back into culture to form more plants.

chong · 06-25-2008, 01:06 AM

Looking good Gabe! How long has it been since you started them up to this point? And what variety(ies) is (are) this?

Gabe15 · 06-25-2008, 01:14 AM

They are 6 days old. The close up ones are of Little Prince, and on the shot of the vessels the front left 4 are Little Prince (from the same plant), the right one is Musella lasiocarpa, and the stacked ones in the back are M. ingens and E. perrieri embryos, M. laterita and an unknown Musa.

tony palmer · 06-25-2008, 02:09 AM

Gabe,
Thats fascinating stuff, i know very little about tissue culture, and have always assumed it would be vey scientific technical as well as expensive to set up, is there such a thing as a home TC kit? and is it expensive to set up, i am looking forward to seeing how your TC bananas come on.

Thanks Tony

tony palmer · 06-25-2008, 04:00 AM

Sorry Gabe I have just seen Kev’s post on the subject.

Mark Hall · 06-25-2008, 04:29 AM

Nice one Gabe. If the Ingens take off you will be very popular with all the members on here ( not that you arn't anyway)

Good luck with them. I shall follow this thread with interest

griphuz · 06-25-2008, 06:43 AM

Interesting Gabe!
Is it a lot of work to do, and could you tell me something about the hormone-works?
Do you forst add hormones to invoce rootgrowth, and next different hormones to invoke leaf and stem growth or how does that work?
Kind regards,
Remko.

Bananaman88 · 06-25-2008, 07:17 AM

Looking good, Gabe! Our resident Mad Scientist! Keep up the good work!

Chironex · 06-25-2008, 09:25 AM

Hey, way to go Gabe! Where did you find that hydrometer/thermometer? Looks like it would be handy for my TC lab - once I get started.

"Dibs" on the Ingens! lol

mskitty38583 · 06-25-2008, 11:33 AM

gabe is this your first try with the home tc? i think that is so very interesting! congrats on your new babies.

Dean W. · 06-25-2008, 11:41 AM

Way to go!

momoese · 06-25-2008, 12:31 PM

Very cool! Was it difficult?

hydrojeff · 06-25-2008, 01:30 PM

super sweet Gabe, thats awsome, i bought the book i think you were talking about, PLANTS FROM TESTTUBES, just started reading it, very interesting stuff.

damaclese · 06-25-2008, 05:34 PM

thanks Gabe ones again you continue to impress me thank you for sharing that and please don't hesitate to keep informing us i cant tell you how fascinating it is to read about TC maybe some day we can send you some TC of ares!
Pauly

TracyWV · 06-25-2008, 09:30 PM

Quote:

Originally Posted by Chironex

Hey, way to go Gabe! Where did you find that hydrometer/thermometer? Looks like it would be handy for my TC lab - once I get started.

"Dibs" on the Ingens! lol

You can find them in pet shops in the reptile section.

Chironex · 06-25-2008, 09:31 PM

I am almost finished with the book and have all of the chemicals ordered. We just need to make a laminar flow hood. Gabe sent me the instructions on how he made his, so we will be ready to go once I get there.
If you can round up couple of quart sized jars, wide-mouthed, (like Mason canning jars or a mayonnaise jar). Also, need some 4 or 6 oz baby food jars we should have just about everything we need. I have about 30 of them, but we will need a bunch eventually.

I am so excited to begin, I can't stand it!

banana berserker · 06-25-2008, 09:42 PM

that looks very intriguing! I'm stupid when it comes to tissue culturing. I've no clue what so ever of how to. can anyone explain please?

Bananaman88 · 06-26-2008, 07:20 AM

Berserker,

Use the search feature and you'll find several threads on this subject. Gabe has a really good diagram illustrating how you start with one plant and end up with many. It's in another post. You can also search his photo gallery, which contains that diagram as well.

51st state · 06-26-2008, 03:29 PM

excel there Gabe, keep 'em going. you got the Ingens to work then

. I'd better think of some more exotic varieties for you to play with, 'Xishuangbannensis' maybe? or Musa Sp. Yangtse (whatever it is?).
regards
Kev

Gabe15 · 06-26-2008, 09:06 PM

Quote:

06-25-2008, 12:46 AM	#1 (permalink)
Gabe15 MC Banana Commander Gabe15's Photo Gallery Location: Honolulu, HI Find me on the map! Zone: 11 Join Date: Jul 2005 Posts: 1,545 BananaBucks : 17,291 Feedback: 5 / 100% Thanks: 1 Thanked 795 Times in 332 Posts Welcomes: 1	My home tissue culture progress These are some photos of my first home grown tissue cultures, which have been growing for 6 days. You can see they have turned green and are starting to grow tiny leaves. When they grow large enough and start to form real leaves, pseudostems and pups (in a few more weeks), I can separate them and put them back into culture to form more plants. __________________ The only hemp Im growing is Manila.

06-25-2008, 01:06 AM	#2 (permalink)
chong Senior Member chong's Photo Gallery Location: Seattle, WA Find me on the map! Zone: 8-9 Name: Chong Join Date: Nov 2006 Posts: 930 BananaBucks : 6,704 Feedback: 3 / 100% Thanks: 170 Thanked 375 Times in 222 Posts Welcomes: 232	Re: My home tissue culture progress Looking good Gabe! How long has it been since you started them up to this point? And what variety(ies) is (are) this?

06-25-2008, 01:14 AM	#3 (permalink)
Gabe15 MC Banana Commander Gabe15's Photo Gallery Location: Honolulu, HI Find me on the map! Zone: 11 Join Date: Jul 2005 Posts: 1,545 BananaBucks : 17,291 Feedback: 5 / 100% Thanks: 1 Thanked 795 Times in 332 Posts Welcomes: 1	Re: My home tissue culture progress They are 6 days old. The close up ones are of Little Prince, and on the shot of the vessels the front left 4 are Little Prince (from the same plant), the right one is Musella lasiocarpa, and the stacked ones in the back are M. ingens and E. perrieri embryos, M. laterita and an unknown Musa. __________________ The only hemp Im growing is Manila.

06-25-2008, 02:09 AM	#4 (permalink)
tony palmer Junior Member tony palmer's Photo Gallery Join Date: Jan 2006 Posts: 116 BananaBucks : 2,357 Feedback: 0 / 0% Thanks: 13 Thanked 45 Times in 26 Posts Welcomes: 0	Re: My home tissue culture progress Gabe, Thats fascinating stuff, i know very little about tissue culture, and have always assumed it would be vey scientific technical as well as expensive to set up, is there such a thing as a home TC kit? and is it expensive to set up, i am looking forward to seeing how your TC bananas come on. Thanks Tony

06-25-2008, 04:00 AM	#5 (permalink)
tony palmer Junior Member tony palmer's Photo Gallery Join Date: Jan 2006 Posts: 116 BananaBucks : 2,357 Feedback: 0 / 0% Thanks: 13 Thanked 45 Times in 26 Posts Welcomes: 0	Re: My home tissue culture progress Sorry Gabe I have just seen Kev’s post on the subject.

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06-25-2008, 04:29 AM	#6 (permalink)
Mark Hall Mark Mark Hall's Photo Gallery Location: Windsor u.k. Find me on the map! Name: Mark Hall Join Date: Oct 2006 Posts: 438 BananaBucks : 5,065 Feedback: 1 / 100% Thanks: 91 Thanked 61 Times in 37 Posts Welcomes: 2	Re: My home tissue culture progress Nice one Gabe. If the Ingens take off you will be very popular with all the members on here ( not that you arn't anyway) Good luck with them. I shall follow this thread with interest __________________ Time Flies like an Arrow.....Fruit flies like a Banana.

06-25-2008, 06:43 AM	#7 (permalink)
griphuz Junior Member Location: Netherlands (Zone 8) Find me on the map! Zone: 7 - 8a Name: Remko Join Date: Sep 2006 Posts: 77 BananaBucks : 1,833 Feedback: 0 / 0% Thanks: 16 Thanked 28 Times in 14 Posts Welcomes: 217	Re: My home tissue culture progress Interesting Gabe! Is it a lot of work to do, and could you tell me something about the hormone-works? Do you forst add hormones to invoce rootgrowth, and next different hormones to invoke leaf and stem growth or how does that work? Kind regards, Remko.

06-25-2008, 07:17 AM	#8 (permalink)
Bananaman88 Bananaculturist Bananaman88's Photo Gallery Location: Pearland, TX Find me on the map! Zone: 9 Name: Brent Join Date: Jun 2007 Posts: 1,104 BananaBucks : 8,833 Feedback: 15 / 100% Thanks: 113 Thanked 311 Times in 214 Posts Welcomes: 8	Re: My home tissue culture progress Looking good, Gabe! Our resident Mad Scientist! Keep up the good work!

06-25-2008, 09:25 AM	#9 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,227 BananaBucks : 544 Feedback: 5 / 100% Thanks: 1,910 Thanked 323 Times in 226 Posts Welcomes: 251	Re: My home tissue culture progress Hey, way to go Gabe! Where did you find that hydrometer/thermometer? Looks like it would be handy for my TC lab - once I get started. "Dibs" on the Ingens! lol __________________ Scot "Grow 'em if ya got 'em!" Last edited by Chironex : 06-25-2008 at 09:25 AM. Reason: because I forgot to claim dibs

06-25-2008, 11:33 AM	#10 (permalink)
mskitty38583 banana junkie mskitty38583 's Photo Gallery Location: sparta, tn. Find me on the map! Zone: 6-7 Name: mskitty Join Date: Nov 2007 Posts: 3,598 BananaBucks : 16,920 Feedback: 24 / 100% Thanks: 23 Thanked 466 Times in 396 Posts Welcomes: 59	Re: My home tissue culture progress gabe is this your first try with the home tc? i think that is so very interesting! congrats on your new babies. __________________ WELCOME TO THE GATOR NATION "kokomo" beach boys:

06-25-2008, 11:41 AM	#11 (permalink)
Dean W. Dean W.'s Photo Gallery Location: Cedar Park, TX Zone: 8b Name: Dean Join Date: Sep 2007 Posts: 1,199 BananaBucks : 8,178 Feedback: 6 / 100% Thanks: 645 Thanked 223 Times in 210 Posts Welcomes: 199	Re: My home tissue culture progress Way to go! __________________ Dean

06-25-2008, 12:31 PM	#12 (permalink)
momoese Organic Mechanic momoese's Photo Gallery Location: West Los Angeles CA Find me on the map! Zone: zone 10 Name: Mitchel Join Date: Aug 2005 Posts: 1,983 BananaBucks : 30,152 Feedback: 9 / 100% Thanks: 36 Thanked 380 Times in 207 Posts Welcomes: 91	Re: My home tissue culture progress Very cool! Was it difficult? __________________ Mitchel

06-25-2008, 01:30 PM	#13 (permalink)
hydrojeff Bananacitus Location: orange city,FL Find me on the map! Zone: zone 9b Name: jeff Join Date: Oct 2007 Posts: 250 BananaBucks : 1,322 Feedback: 8 / 100% Thanks: 85 Thanked 39 Times in 38 Posts Welcomes: 98	Re: My home tissue culture progress super sweet Gabe, thats awsome, i bought the book i think you were talking about, PLANTS FROM TESTTUBES, just started reading it, very interesting stuff. __________________ Jeff

06-25-2008, 05:34 PM	#14 (permalink)
damaclese Living in Exile damaclese's Photo Gallery Location: Henderson NV Find me on the map! Zone: 9 Name: Pauly Join Date: Apr 2008 Posts: 716 BananaBucks : 6,792 Feedback: 0 / 0% Thanks: 381 Thanked 249 Times in 187 Posts Welcomes: 41	Re: My home tissue culture progress thanks Gabe ones again you continue to impress me thank you for sharing that and please don't hesitate to keep informing us i cant tell you how fascinating it is to read about TC maybe some day we can send you some TC of ares! Pauly

06-25-2008, 09:31 PM	#16 (permalink)
Chironex Dayyyyyyyy-O Chironex's Photo Gallery Location: Las Vegas, NV Find me on the map! Zone: 9 Name: Scot Join Date: Apr 2008 Posts: 1,227 BananaBucks : 544 Feedback: 5 / 100% Thanks: 1,910 Thanked 323 Times in 226 Posts Welcomes: 251	Re: My home tissue culture progress I am almost finished with the book and have all of the chemicals ordered. We just need to make a laminar flow hood. Gabe sent me the instructions on how he made his, so we will be ready to go once I get there. If you can round up couple of quart sized jars, wide-mouthed, (like Mason canning jars or a mayonnaise jar). Also, need some 4 or 6 oz baby food jars we should have just about everything we need. I have about 30 of them, but we will need a bunch eventually. I am so excited to begin, I can't stand it! __________________ Scot "Grow 'em if ya got 'em!"

06-25-2008, 09:42 PM	#17 (permalink)
banana berserker Location: sacramento, CA Find me on the map! Zone: 9 Join Date: Jan 2008 Posts: 152 BananaBucks : 4,215 Feedback: 0 / 0% Thanks: 2 Thanked 7 Times in 5 Posts Welcomes: 1	Re: My home tissue culture progress that looks very intriguing! I'm stupid when it comes to tissue culturing. I've no clue what so ever of how to. can anyone explain please?

06-26-2008, 07:20 AM	#18 (permalink)
Bananaman88 Bananaculturist Bananaman88's Photo Gallery Location: Pearland, TX Find me on the map! Zone: 9 Name: Brent Join Date: Jun 2007 Posts: 1,104 BananaBucks : 8,833 Feedback: 15 / 100% Thanks: 113 Thanked 311 Times in 214 Posts Welcomes: 8	Re: My home tissue culture progress Berserker, Use the search feature and you'll find several threads on this subject. Gabe has a really good diagram illustrating how you start with one plant and end up with many. It's in another post. You can also search his photo gallery, which contains that diagram as well.

06-26-2008, 03:29 PM	#19 (permalink)
51st state Mimi's dad's grown a Nana 51st state's Photo Gallery Location: salisbury, UK Find me on the map! Zone: 8b ish Name: Kev Join Date: Nov 2006 Posts: 281 BananaBucks : 7,797 Feedback: 2 / 100% Thanks: 50 Thanked 67 Times in 46 Posts Welcomes: 0	Re: My home tissue culture progress excel there Gabe, keep 'em going. you got the Ingens to work then . I'd better think of some more exotic varieties for you to play with, 'Xishuangbannensis' maybe? or Musa Sp. Yangtse (whatever it is?). regards Kev __________________ a>

06-26-2008, 09:06 PM	#20 (permalink)
Gabe15 MC Banana Commander Gabe15's Photo Gallery Location: Honolulu, HI Find me on the map! Zone: 11 Join Date: Jul 2005 Posts: 1,545 BananaBucks : 17,291 Feedback: 5 / 100% Thanks: 1 Thanked 795 Times in 332 Posts Welcomes: 1	Re: My home tissue culture progress Quote: