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-   -   How to care for tissue cultures or young plants. (http://www.bananas.org/f2/how-care-tissue-cultures-young-plants-20017.html)

PR-Giants 03-22-2014 02:20 PM

How to care for tissue cultures or young plants.
 
:woohoonaner:

pniksch 03-22-2014 06:29 PM

Re: How to care for tissue cultures or young plants.
 
Who is the grower, and have you purchased from them before?
Glad to see they're hanging in there and thriving!
Thanks,
Paul

bananimal 03-22-2014 07:56 PM

Re: How to care for tissue cultures or young plants.
 
Supplier is USDA ars - grin I believe. The PO made them what the are later on arrival. I can't get them - I'm not a commercial grower.

Abnshrek 03-22-2014 08:18 PM

Re: How to care for tissue cultures or young plants.
 
Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)

PR-Giants 03-22-2014 10:39 PM

Re: How to care for tissue cultures or young plants.
 
Quote:

Originally Posted by Abnshrek (Post 241428)
Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)

In the Tropics germplasm are actually very strong, all of the plantlets survived and a few produced sister shoots. Being prepared and having confidence in your ability sure helps. The people who have problems are generally adding too much crap too early, like fish emulsion, azomite, mycorrhizae, and fertilizer. If spending more money will help the plant grow then simply buy a jewel encrusted Tiffany spray bottle.

I chose to set the sleeves so that the germplasm received full morning sun from the very first day and waited about 10 to 12 days before removing the sleeves completely. By hardening the plants more quickly they can grow faster by using the full sun.

This photo was posted on Feb-15, it is of a fully hardened Tigua :ha: height 0.18".

Quote:

Originally Posted by PR-Giants (Post 227418)

Super tall fully hardened TC


Abnshrek 03-22-2014 10:43 PM

Re: How to care for tissue cultures or young plants.
 
That is a benefit of being in Banana Heaven.. :^)

ez 03-22-2014 10:54 PM

Re: How to care for tissue cultures or young plants.
 
Quote:

Originally Posted by Abnshrek (Post 241428)
Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)

It generally takes 4 to 5 weeks for plantlets that are removed from culture vessels to reach this size, after the initial hardening process (acclimatization) they will be transferred to a greenhouse.



They will need a secondary hardening process for two months in a greenhouse before they can be planted in the field.
__________________

Abnshrek 03-22-2014 11:03 PM

Re: How to care for tissue cultures or young plants.
 
Quote:

Originally Posted by ez (Post 241440)
It generally takes 4 to 5 weeks for plantlets that are removed from culture vessels to reach this size, after the initial hardening process (acclimatization) they will be transferred to a greenhouse.



They will need a secondary hardening process for two months in a greenhouse before they can be planted in the field.
__________________

That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)

ez 03-22-2014 11:12 PM

Re: How to care for tissue cultures or young plants.
 
Quote:

Originally Posted by Abnshrek (Post 241441)
That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)

Do they give you instructions on how to take care of them? :waving:

ez 03-22-2014 11:58 PM

Re: How to care for tissue cultures or young plants.
 
Quote:

Originally Posted by Abnshrek (Post 241441)
That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)

Quote:

Originally Posted by ez (Post 241442)
Do they give you instructions on how to take care of them? :waving:

Quote:

Originally Posted by PR-Giants (Post 241446)
If you follow the instructions from the tissue culture lab, U. S. Department of Agriculture Tropical Agriculture Research Station, your plants are acclimatized in about 2 weeks. I probably saved the instruction sheet, but it matched perfectly to what I observed.

I meant instructions from wellspring gardens do they send you instructions with the plants.

:2738: I've read technical papers on the subject saying it would take three weeks or more.
Your technique of two weeks for hardening is impressive.:08:

ez 03-23-2014 01:12 AM

Re: How to care for tissue cultures or young plants.
 
Quote:

Originally Posted by PR-Giants (Post 241455)

The most noticeable difference with your photo is that the plant is tall with small leaves,

and mine are short with large leaves, it would be nice to know the cultivar.

They don't say which cultivar

Preparing Tissue-Cultured Banana Plantlets for Field Planting - pdf

http://www.cialca.org/files/files/extension_materials/tissue-culture_english.pdf

Abnshrek 03-23-2014 11:41 AM

Re: How to care for tissue cultures or young plants.
 
Quote:

Originally Posted by PR-Giants (Post 241470)
Dan, u don't need no GRIN, u's got friends.

Shrek is sending u a Datil and I'll send u a Patupi the following year,

this way u can harvest both at the same time.

That's right.. Mine should be big enough to flourish this year.. :^)

@ Keith We know how you ( a professional banana grower grows), but you have to factor in people that have no experience growing banana's, and will just buy dirt thinking its all the same, which isn't so.. water crystals or water retainers in soil is one way to kill any banana quick up here, and certainly anywhere North of where I am.

Nicolas Naranja 03-27-2014 08:36 PM

Re: How to care for tissue cultures or young plants.
 
I put the plants from GRIN underneath a bench that gets misted for about 2 weeks and then I place them on top of the bench.

For agristarts bananas, I put them in partial shade in a wind-protected location for 2 weeks and then move them out.


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