Zac in NC

Yes, any updates? I am goign to Plant Delights this afternoon and I will ask Tony if he has this one yet. I'll let you know what I find out.

Zac

Basjoofriend

And also to Austria and Germany? One friend of me need this.

Ryan, please email me! I'm still waiting for one reply from you because of the original full version of the pics of fruits of Ae Ae Variegata, Musa siamensis and Musa 'Palawan Red Variegated' for my book and some plants! I have to complete my book as soon as possible! Time is money! Please go online again, look at emails and go to our forum and look at your PN and postings!

Best thanks in advance!

Best wishes
Joachim

kgbenson

"I know very little about tissue culture to do them myself and I am very ambivalent about turning it over to the big TC operations."

TC is not terribly challenging with bananas. I would go for it. you can get into it for <100 dollars.

Keith

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http://www.geocities.com/k2benson/TC...ban/TCban.html

MediaHound

How would you go about getting into it on a level like that?

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kgbenson

Check out the following: The page is being re-constructed so there may be a few dead links.

http://www.geocities.com/k2benson/TC...ban/TCban.html

http://www.geocities.com/k2benson/TC...a-explant.html

The above are for the "at home" version.

Iam McGuire has a nice website at: http://litzlab.ifas.ufl.edu/banana.htm He did hie stuff in a lab, but frankly there is nothing he shows that cannot be done in your kitchen - and with some of the new media formulations, most of the scales, pH meters etc. are superfluous. He has terrific photos.

There is a group of fine folks who do home TC: http://www.kitchenculturekit.com/index.htm

THe media can be purchased inexpensively - with all of the eingredients already mixed in, so no messy chemistry:
http://www.phytotechlab.com/TABSTYLE/searchresult.asp?CartId={10502052-E396-432F-A91A-5836D65EVEREST59A40}
You do not need laminar flow hoods and such.

Keith

__________________
Bee Sting Honey . . . So Good It Hurts
http://www.geocities.com/k2benson/TC...ban/TCban.html

Zac in NC

I would hesitate to TC at home. I know that the Botany Department at my university had trouble with getting it to work for their student labs. Contamination is a big problem. And this was trying to TC easy things, like Pelargoniums I believe. I think if I wanted it TC'd, I would see if I could find a nurseryman who would have it done for me, much like I am doing with a variegated Agave which Tony Avent now has.

With mentioning him, I did ask him if he had any of this banana, and he told me that none of the ones he brought back had made it. That is pretty sad, but I think he'll have it eventually.

Zac

kgbenson

It is easier than you think - contamination is an occasional problem, but I think I had less than a 1% contmaination rate.

Keith

__________________
Bee Sting Honey . . . So Good It Hurts
http://www.geocities.com/k2benson/TC...ban/TCban.html

MediaHound

Thanks for the links, Keith -
The last one was an expired shopping cart. Could you post the items you were linking to? They seem to have an extensive product line on their homepage.

JoeReal did a thread on TC a while back
Micropropagation of bananas

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kgbenson

Sure can. Just type Musa into the search engine at Phytotech.

The stuff you want ior initiation and propagation is:

M462-10L MUSA (BANANA) MULTIPLICATION MEDIUM IITA FORMULATION AS DESCRIBED BY VUYLSTEKE (1989). PLANT TISSUE CULTURE TESTED

Then just get some MS media without the BA for rooting.

If you want you can buy a single liter of any media - but it is best to buy 10 1 Liter bottles. Just add distilled water and serilize. Everything is pre-measured.

Also - while I am editing, the following two very inexpensive publications are excellent. They contain all the facts, and nothing but the facts for propagating and post-flask management of Musa.

http://www.iita.org/info/spread.asp?id=TE/125

http://www.iita.org/info/spread.asp?id=TE/127

I am updating my website - should have better pics and more detail over the next few weeks.



Keith

__________________
Bee Sting Honey . . . So Good It Hurts
http://www.geocities.com/k2benson/TC...ban/TCban.html