eaperez

Hello to everyone,
My name is Eden A. Perez. I am from the Philippines and I'm here at the University of Hawaii since 1999. I graduated in 2006 with Ph. D. in Plant Pathology (Virology). I am currently working on my project entitled "Rehabilitation of BBTV infected areas in Hawaii" thus my work involves indexing bananas for use as starting material of in vitro cultures and micropropagation of virus-free bananas. Hope to meet all of the members through chat here. I welcome questions regarding tissue culture and genetic improvement of bananas. Aloha......Eden

xavierdlc61887

u should meet "GABE" hes in hawaii too and does what u do!!!!

mskitty38583

welcomoe to the org....ok question number 1: in laymans terms please....why cant we tc an aeae and get a varigated species??????????????

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WELCOME TO THE GATOR NATION

eaperez

Hi to all,
Thanks so much for welcoming me.......eden

eaperez

sorry, i think i don't know an aeae. is this a varigated banana? i think i have an idea if it is.....eden

eaperez

know what an aeae is.

sorry, i replied to all. now i discovered how to send a reply to each of the emails. i do not know what an aeae is. if it is varigated banana, i think i know why it cannot be tc to produce more varigated plantlets.....

eaperez

i know one Gabe. i think he is one and the same Gabe who is one of my lab assistants.....

xavierdlc61887

look for him here maybe it is!!!

Caloosamusa

Welcome to Bananas dot org Dr. Perez,

If we can help you in any way just ask if you can't find it on the threads. I will be asking you many questions about plant pathology, especially mangos, and some other plants. I know your focus may be viruses, but I know you know more than we do about plant pathogens.

Best wishes and welcome to the organization!!

Taylor

Welcome Eden.
His username is Gabe15. And yes, he is a lab assistant. I'd gather that it is the same person!
Here he is:
Gabe's Member Profile

Thanks for all the work you are doing to produce virus-free crops in Hawaii.

eaperez

I looked at Gabe15's profile and found out that he's my lab assistant. i will tell him about this tomorrow.
thanks,
eden

Taylor

It's a small world!

Any information you will share with us here in your visits will be greatly appreciated.

eaperez

Thanks. I'll try to give you the best answer on all your questions. If I didn't answer today, you'll get them the next day. it is now 4:10 pm here (HI time) and will continue my work till 6:00 pm.

buzzwinder

Welcome to the Org. Eden, Glad you joined us, and looking forward to your insites on some of our members questions, this is and has been a GREAT place to learn about Banana culture, as well as alot of humor tossed in for good measure!

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Bill for my friend

conejov

Welcome!

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eaperez

Thanks!!!!
eden

Chironex

Hi Eden! I am particularly glad that you joined us here. As an amateur tissue culturer I am happy to be able to learn more about new studies and sharing of techniques.
I have been experiencing several challenges in embryo rescue, finding a good medium to promote growth. I would also like to find out a technique for some of the Ensete varieties such as ventricosum Maurelii and perrieri. Apparently there has been little success with traditional M&S medium with Ensete. I may also have access to E. v. 'Montebeliardii' which is far more rare than 'Maurelii'. I have read some research on a newer medium, but wondered about any experience that you may have.
Welcome to Bananas.org!

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Scot

eaperez

Aloha! thanks to your message. i have not done any embryo rescue on bananas. and i would like to direct your inquiries to Gabe15 (also a member and one of my lab assistants) whom i know has been doing a lot of embryo rescues in our lab for his own research. i only do micropropagation of bananas right now and i assume embryo rescue should not be difficult unless the seeds are not viable. are you excising the embryos from the seed? are those seeds mature or immature? by the way, are you doing research? i have been doing tissue culture for more than 30 years on a lot of different crops. i am willing to give you some advice on your next mail. thanks,
eden

Chironex

Thanks Eden, yes I have talked at length with Gabe about the struggles. In particular we have been trying to rescue embryos from M. ingens. They seem to have an endogenous contamination issue. I was going to attempt surface sterilization of the seeds, prior to excision, using silver nitrate as described by Dr Frederic Bakry. But I believe that this still will not cure the issue of endogenous contamination. I have used standard surface sterilization using bleach solutions, physan 20 and hydrogen peroxide. Every time, I either get contamination, or no growth. I have also tried modified N&N medium with the additions of kinetin and also ethrel. Still nothing. I am wondering if I should increase the percentage of PPM in the medium? I was also curious whether you thought it would help by placing the embryos directly into a PPM soak prior to placing them onto the medium? Would this tend to kill the embryo? I don't have much luck with PPM so far.

I did a lot of research, particularly with sharks and cubomedusae when I was at the U of Miami in 1976, but not much since then. I worked with Dr Samuel Gruber. I was pre-med with a Biology major. Began working on my thesis, but due to family medical issues, had to withdraw from continuing my education. I miss research as you can probably sense! Quite a bit rusty, but nonetheless, the fire still burns.

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Scot

Tog Tan

Hiya Eden and welcome to the board from the other side of the world! It's great to have an expert to lead this bunch of crazy 'naner nuts.

Looking forward to learn more from you. Have a good day!

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Tog Tan has sadly suddenly passed away 6/16/09. We will miss you Tog Tan.