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how to sterilized banana tissue culture
hi there .. i am new here and i want a discussion regarding sterilization and protocols of banana tissue cukture.. i hope you guys will share some iddeas...
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kubali
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PURA VIDA!!
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to sterilize the medium I use a pressure cooker for my orchid media when I flask them..i have had success in oven,but easer to use pressure cooker...tissue itself..have here 10% bleach 3% hydrogen peroxide mix will surface sterilize..i use a diy glovebox I made from plywood and plastic sheet..i do also use a homebrew agar media of my own recipe...potato,suger,young coconut water,pineapple juice,charcoal,dash of fertilizer,agar powder..
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is there a banana enthusiast here knew on how to sterilize to eliminate contamination on tissue culture
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