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View Full Version : Step by Step Subculture In Tissue CUlture Bananas (part 1)


novisyatria
10-07-2009, 03:14 AM
Hi all
I promise you all to take photo subculture in TC bananas step by step. thats why i I'v done today. enjoy the pic.

Step 1. Explant banana 1month old. ready for subculture 1 (Photo 1)
Step 2. Put the explant on sterile petri dish/plate (Photo 2)
Step 3. Cut vertically become 2 explant (Photo 3)
Step 4. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo4)
Step 5. After 1 month next, explant has grow and ready for subculture 2. explant will have few baby bananas. about4-7 baby (photo 5)
Step 6. Put the explat on sterile petridish (Photo 6)
Step 7. Cut the explant become few. every parts could be consist of 2-3 baby bananas (Photo 7)
Step 8. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo8). Look at the next thread
Next Step like subculture 1 or subculture 2

Jack Daw
10-07-2009, 11:18 AM
A thread many of us have been waiting for. Thanks for this one, pal.

r3tic
10-07-2009, 12:11 PM
That's great!....Thanks for taking the time to share the information and photos.

griphuz
10-07-2009, 04:27 PM
Very Nice!
Could you give us a little more detail though about each stage, and how you got there, and to the next stage?

Kind regards,
Remko.

novisyatria
10-07-2009, 07:20 PM
Hi Remko
before this thread i hav been post step by step how TC bananas. just look in TIssue Culturing Forum jump

regrads

ArchAngeL01
10-07-2009, 08:41 PM
sweet@!

xmoose
10-09-2009, 02:17 PM
Awesome !!!:02:

Indrani Chetia
04-24-2019, 10:04 PM
I am facing problems in the shoot multiplication step.Can you please help me out why there is no growth even after adding adequate hormones and frequent subcultures?

novisyatria
04-30-2019, 09:03 PM
I am facing problems in the shoot multiplication step.Can you please help me out why there is no growth even after adding adequate hormones and frequent subcultures?

hi my WA no +62811732212

thanks

Bananagardendreamboy
05-19-2020, 10:20 AM
Nice photos, pretty remarkable this plant can be divided so early and often