Log in

View Full Version : Medium MS Organic as Substitute Medium MS


novisyatria
09-17-2009, 06:06 AM
sometime is hard to get or buy media MS for tissue culture. I want to share alternative media as substitute media MS. we called its Media MS organic. I hope this information will helpning you that interested with TC

Ingredient:
- Aquades 500 ml
- Coconut water 200 ml
- 4 gr fertilizer NPK 20 : 20 : 20 (like vitabloom, growmore, hyponex)
- Sugar 40 gr
- 7 gr seaweed (1 sachet)
- 500 gr bean spouts, mix in blender, filter (for rooting medium). For shoot medium change bean sprouts with stringbean
Methods
- Mix fertilizer, sugar, seaweed and bean sprout concentrate, coconut water in to baker glass ( 1 L). mix until homogenuos
- Add aquades until solution became 1 L
- Do PH 5,8
- Boiled the solution until boiling, put in the culture botol. Each bottle 25-30 ml solution. And autoclaving. wait medium until cold and ready for use


regards

novisyatria
09-17-2009, 07:20 AM
see picture tissue culture bananas growing in MS Organic medium. see in my gallery (IMGP2152_TC_Bananas_in_MS_Organic_medium).
i think its expalnt growth good enough in media substitude

jmoore
09-17-2009, 09:56 AM
That's marvellous!

Can I just check that where it says seaweed, does that mean agar?

novisyatria
09-17-2009, 10:18 AM
yes sewees = agar

xavierdlc61887
12-29-2009, 11:12 PM
Ok i did the media just as novi mentions and here is a bottle with the media

http://i84.photobucket.com/albums/k9/xavierdlc61887/p_00268.jpg

I will be doing some explants, using this organic medium so be on the look out on progress!

novisyatria
12-29-2009, 11:47 PM
cool Fernando. hope you succes in TC