Log in

View Full Version : New to the forum - (Tissue Culturing Banana Plants)


MurCurY
10-18-2008, 10:17 AM
Hello world.

I work at a tissue culture lab that does small fruit here in the US. I actually work in the greenhouse as Assistant Grower. we harden off the plants that come out of TC stage II media into plugs that are field planted.

My true love for the lab stems from my love of fungi. After building my flowhood and learning sterile technique and agar.....i expanded my knowledge from microscopic tissue culture of spores and mycellium...into something slightly larger.....multicellular organisms.

I found a local greenhouse that sold banana plants....that they tissue cultured. I purchased a few...Super Dwarf Cavendish, Rajapuri, and Velutina, with the hopes of enjoying a few fruit....maybe......
Now I feel I should do something with them....like TC!
2 plants are throwing pups. I plan on removing and cleaning them tomorrow. I'm going to search around the site....however....if someone has pictures of how large a pup has to be before removing for culture...THAT WOULD BE GREAT!

I have NAA and 6-BA that I plan on using with MS media in ball jars.
I order my chemicals through work...Sigma plant cell culture section.


Well.....here I am. I'll post some pics of my hood at some point.

john_ny
10-18-2008, 10:20 AM
Looking forward to hearing more from you.

Tog Tan
10-18-2008, 01:05 PM
Welcome to the 'naner world. It will be nice to hear from your work on 'naner TC.

MediaHound
10-18-2008, 02:00 PM
Welcome aboard!

D_&_T
10-19-2008, 11:10 AM
Welcome aboard!

We are talking about trying TC at home!

Chironex
10-19-2008, 11:27 AM
Great to have you join us! Having experience in a TC lab is an added bonus. Those of us doing home TC benefit from your experience.

mike
10-19-2008, 07:25 PM
Hello and welcome aboard.

Chironex
01-21-2009, 02:39 AM
The smallest pup that I have TC'ed so far was about 12-14" long. I was able to get the apical meristem quite easily. It is ready for its third sub-culture now.

mskitty38583
01-21-2009, 08:42 AM
welcome to the org....i havent tc anything...well at least not yet..i have my eyes on 2 types of nanas i would like to try putting together.....:lurk:

buzzwinder
01-21-2009, 12:09 PM
Welcome to the Org. :bananas_b

MurCurY
02-27-2009, 03:10 PM
ok so....with things at work getting hectic i didn't get to the bananas when i wanted. I'm sick...so....tomorrow i'm going to disinfect and plate up a pup.

Caloosamusa
02-27-2009, 04:28 PM
Welcome aboard!:bananaflipflop:

I look forward to you insights and experience!! :7):2239: