View Full Version : My home tissue culture progress
Gabe15
06-24-2008, 11:46 PM
These are some photos of my first home grown tissue cultures, which have been growing for 6 days. You can see they have turned green and are starting to grow tiny leaves. When they grow large enough and start to form real leaves, pseudostems and pups (in a few more weeks), I can separate them and put them back into culture to form more plants.
http://www.bananas.org/gallery/watermark.php?file=10677&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=10677&ppuser=5)
http://www.bananas.org/gallery/watermark.php?file=10675&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=10675&ppuser=5)
http://www.bananas.org/gallery/watermark.php?file=10676&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=10676&ppuser=5)
http://www.bananas.org/gallery/watermark.php?file=10678&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=10678&ppuser=5)
chong
06-25-2008, 12:06 AM
Looking good Gabe! How long has it been since you started them up to this point? And what variety(ies) is (are) this?
Gabe15
06-25-2008, 12:14 AM
They are 6 days old. The close up ones are of Little Prince, and on the shot of the vessels the front left 4 are Little Prince (from the same plant), the right one is Musella lasiocarpa, and the stacked ones in the back are M. ingens and E. perrieri embryos, M. laterita and an unknown Musa.
tony palmer
06-25-2008, 01:09 AM
Gabe,
Thats fascinating stuff, i know very little about tissue culture, and have always assumed it would be vey scientific technical as well as expensive to set up, is there such a thing as a home TC kit? and is it expensive to set up, i am looking forward to seeing how your TC bananas come on.
Thanks Tony
tony palmer
06-25-2008, 03:00 AM
Sorry Gabe I have just seen Kev’s post on the subject.:raftingnaner:
Mark Hall
06-25-2008, 03:29 AM
Nice one Gabe. If the Ingens take off you will be very popular with all the members on here ( not that you arn't anyway)
Good luck with them. I shall follow this thread with interest:waving:
griphuz
06-25-2008, 05:43 AM
Interesting Gabe!
Is it a lot of work to do, and could you tell me something about the hormone-works?
Do you forst add hormones to invoce rootgrowth, and next different hormones to invoke leaf and stem growth or how does that work?
Kind regards,
Remko.
Bananaman88
06-25-2008, 06:17 AM
Looking good, Gabe! Our resident Mad Scientist! Keep up the good work!
Chironex
06-25-2008, 08:25 AM
Hey, way to go Gabe! Where did you find that hydrometer/thermometer? Looks like it would be handy for my TC lab - once I get started.
"Dibs" on the Ingens! lol
mskitty38583
06-25-2008, 10:33 AM
gabe is this your first try with the home tc? i think that is so very interesting! congrats on your new babies.:2791:
Dean W.
06-25-2008, 10:41 AM
Way to go! :2126:
momoese
06-25-2008, 11:31 AM
Very cool! Was it difficult?
hydrojeff
06-25-2008, 12:30 PM
super sweet Gabe, thats awsome, i bought the book i think you were talking about, PLANTS FROM TESTTUBES, just started reading it, very interesting stuff.
damaclese
06-25-2008, 04:34 PM
thanks Gabe ones again you continue to impress me thank you for sharing that and please don't hesitate to keep informing us i cant tell you how fascinating it is to read about TC maybe some day we can send you some TC of ares!
Pauly
TracyWV
06-25-2008, 08:30 PM
Hey, way to go Gabe! Where did you find that hydrometer/thermometer? Looks like it would be handy for my TC lab - once I get started.
"Dibs" on the Ingens! lol
You can find them in pet shops in the reptile section. :2698:
Chironex
06-25-2008, 08:31 PM
I am almost finished with the book and have all of the chemicals ordered. We just need to make a laminar flow hood. Gabe sent me the instructions on how he made his, so we will be ready to go once I get there.
If you can round up couple of quart sized jars, wide-mouthed, (like Mason canning jars or a mayonnaise jar). Also, need some 4 or 6 oz baby food jars we should have just about everything we need. I have about 30 of them, but we will need a bunch eventually.
I am so excited to begin, I can't stand it!
banana berserker
06-25-2008, 08:42 PM
that looks very intriguing! I'm stupid when it comes to tissue culturing. I've no clue what so ever of how to. can anyone explain please?
Bananaman88
06-26-2008, 06:20 AM
Berserker,
Use the search feature and you'll find several threads on this subject. Gabe has a really good diagram illustrating how you start with one plant and end up with many. It's in another post. You can also search his photo gallery, which contains that diagram as well.
51st state
06-26-2008, 02:29 PM
excel there Gabe, keep 'em going. you got the Ingens to work then :2748:. I'd better think of some more exotic varieties for you to play with, 'Xishuangbannensis' maybe? or Musa Sp. Yangtse (whatever it is?).
regards
Kev
Gabe15
06-26-2008, 08:06 PM
Very cool! Was it difficult?
Not difficult if you know what you are doing, but without experience may take awhile to get right.
gabe is this your first try with the home tc? babies.:2791:
This is my first time doing it at home, but its my job in Hawaii, so I just took what I learned from there and modified it all to work without a lab and expensive equipment.
Interesting Gabe!
Is it a lot of work to do, and could you tell me something about the hormone-works?
Do you forst add hormones to invoce rootgrowth, and next different hormones to invoke leaf and stem growth or how does that work?
Kind regards,
Remko.
These cultures have in the media the hormone BA (benzyladenine), this suppresses root growth and shoot elongation but encourages shoot proliferation, so you can make multiple meristems on a single tissue mass. Eventually, I will use a very similar media except the BA will be absent, this will allow roots to grow, shoots to elongate, and no longer encourage multiplication, this will allow the growth of single rooted plants ready for transfer to soil.
excel there Gabe, keep 'em going. you got the Ingens to work then :2748:. I'd better think of some more exotic varieties for you to play with, 'Xishuangbannensis' maybe? or Musa Sp. Yangtse (whatever it is?).
regards
Kev
I don't know if they ingens will work or not, I just put them in culture and am in California for a week, so when I get back home next week I will be able to tell if it worked or not.
Chironex
06-30-2008, 06:36 AM
Gabe, please put me on the list for Ingens. Still trying to find some seeds or plants, but no luck so far.
tophersmith
06-30-2008, 11:34 AM
Gabe,
They are looking great. I have a question I received some TC of Siam Ruby this winter, one of which stayed green. Does this mean any pups produced by the TC will also be green or is there a chance of the pups being red?
51st state
06-30-2008, 02:10 PM
Gabe, please put me on the list for Ingens. Still trying to find some seeds or plants, but no luck so far.
Hiya
How many do you want (seeds that is) I supplied gabe with his a couple months back. $2 per seed though I'm afraid, includes shippping though ;)
nucci60
06-30-2008, 02:14 PM
Good for you Gabe, you are in the forefront of the hobby.
Gabe15
06-30-2008, 03:17 PM
Gabe,
They are looking great. I have a question I received some TC of Siam Ruby this winter, one of which stayed green. Does this mean any pups produced by the TC will also be green or is there a chance of the pups being red?
They will likely all stay green.
Gabe15
07-08-2008, 04:27 PM
UPDATE:
Things are going fairly well. The M. ingens seeds did not work (as expected) so I'm still working on that one. Pretty much everything else worked and is growing nicely. I had one unexpected contamination in my Musella vessel, but I may have corrected it (I'll know in a few days).
This 'Little Prince' culture may look like the creature from the black lagoon, but it is actually growing perfectly and is ready to be split into more plants.
http://www.bananas.org/gallery/watermark.php?file=11040&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=11040&ppuser=5)
This Musella lasiocarpa is growing great, but unfortunately there is some bacterial contamination (the off-white goo creeping around the edges). I made up a way to save these kinds of contaminations, but it doesn't work all the time so we shall see if it can be saved.
http://www.bananas.org/gallery/watermark.php?file=11041&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=11041&ppuser=5)
jpfloors
07-08-2008, 04:35 PM
Looks good Gabe! Gabe, I have a request for you, next time you go cutting into a plant to get the meristem would you mind taking pictures to share?!?!? Thanks if you can!
Gabe15
07-08-2008, 06:10 PM
Here is a short video of the actual tissue culture initiation. It starts out with a plant that has been whittled down to a piece of tissue with some pseuodstem above the meristem and some corm below the meristem. The two clips are of two different plants, but nothing is missed in the process. This is part of a video I'm working on detailing the full process.
p4DUwSGJYNE
austinl01
07-08-2008, 06:38 PM
Great work, Gabe. I'm constantly amazed by your banana skills. :2783:
jpfloors
07-08-2008, 06:56 PM
That's awsome Gabe... Now I'm curious though... the last part of the video you had taken the meristem and put it into a type of mason jar, but then you took out something else that you seemed to have been culturing, and put two of the other pieces that you had removed into that one... And what happened with the one you removed? LOL
Gabe15
07-08-2008, 07:15 PM
That's awsome Gabe... Now I'm curious though... the last part of the video you had taken the meristem and put it into a type of mason jar, but then you took out something else that you seemed to have been culturing, and put two of the other pieces that you had removed into that one... And what happened with the one you removed? LOL
In that vessel was an explant of another variety that was growing fine for a few weeks and had no problems, however, instead of using a new vessel, I just took out that explant and put the new ones in since the media was still good and it had no visible contamination. In our lab, if a vessel is contaminated and the plants are large enough, we plant them directly into soil without the normal transfer to the rooting medium to grow roots and leaves while in culture. This doesn't always work, and this explant is on the very smallest end of explants to be used like this, but I figured it was worth a shot to see if it will root in soil.
Here is that explant.
http://www.bananas.org/gallery/watermark.php?file=11042&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=11042&ppuser=5)
http://www.bananas.org/gallery/watermark.php?file=11043&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=11043)
Chironex
07-08-2008, 08:05 PM
This Musella lasiocarpa is growing great, but unfortunately there is some bacterial contamination (the off-white goo creeping around the edges). I made up a way to save these kinds of contaminations, but it doesn't work all the time so we shall see if it can be saved.
Gabe, Curious to know what the contamination is and also the way you are trying to save it.
Chironex
07-08-2008, 08:20 PM
I noticed that the vessels were not ventilated. Do you find that there is less contamination risk this way, or do you transfer to ventilated vessels later on?
Gabe15
07-08-2008, 08:27 PM
Gabe, Curious to know what the contamination is and also the way you are trying to save it.
Its a bacteria. A few times before, I've dipped the explant into the bleach solution, then into the alcohol and burned off all the alcohol and transfered to a new, clean vessel. If done "lightly" enough, I'm sure it damages the outer layers but the inner tissue (where the meristem is) seems to stay protected. I haven't done this very many times, so figured some more experimentation was in order.
I noticed that the vessels were not ventilated. Do you find that there is less contamination risk this way, or do you transfer to ventilated vessels later on?
The box ones allow for gas exchange, normally we seal off the opening with 3M micropore surgical tape (which allows for filtered gas exchange, very useful), however since I forgot to add it to my order, I instead just try to close the lid tightly and then put it in a plastic baggie. The mason jars are probably pretty well sealed (though I do break the tight seal formed by pressure cooking), however, since I'm only leaving these in culture short term, I'm not too worried about it. But then again, its all part of the home TC experimenting, so I'll find out soon enough.
Chironex
07-08-2008, 08:38 PM
Wow, kinda like roasting marshmallows, eh? What dilution of bleach solution, 10% or 20%? I also read where you can use some round band-aids that have a antiseptic gauze to cover a nail hole in the mason jar lids. I think Carol Stiff suggests these as a cheaper alternative than the 3M micropore tape. She says that they will remain in place after autoclaving a few times, too.
Gabe15
07-08-2008, 08:41 PM
Wow, kinda like roasting marshmallows, eh? What dilution of bleach solution, 10% or 20%? I also read where you can use some round band-aids that have a antiseptic gauze to cover a nail hole in the mason jar lids. I think Carol Stiff suggests these as a cheaper alternative than the 3M micropore tape. She says that they will remain in place after autoclaving a few times, too.
Hmmm...interesting on the bandaid bit, I'll add it to my things to test. Thanks for the tip!
I use a ~25% bleach solution, but I just sorta eyed it out to 1/4, no exact measuring.
mskitty38583
07-08-2008, 10:39 PM
gabe i have to say this is interesting stuff. but i also do have to say it looks like something my kitty ate. lol. i really like this genetic stuff.
Chironex
07-08-2008, 10:50 PM
And to think of the money I have spent on graduated cylinders, pipettes and such....oh well. Just my addiction again.
Dean W.
07-08-2008, 10:53 PM
Great video, intresting to see banana plants being made.
harveyc
07-09-2008, 12:02 AM
My 10 year old son enjoyed the video too. But it makes me wonder what your parents think of this. Are the neighbors talking about the strange kid down the block growing bananas in test tubes???? ;)
Gabe15
07-09-2008, 12:16 AM
My 10 year old son enjoyed the video too. But it makes me wonder what your parents think of this. Are the neighbors talking about the strange kid down the block growing bananas in test tubes???? ;)
The neighbors have been saying that for years, haha. My parents are really very supportive with everything. When I first started growing plants, they would kinda oversee everything and limit the amount of plants I kept, but as time went on they realized I could handle it all better then they, and just kinda let me do whatever it is I was doing and left me alone with it. Now with the TC stuff, they are just amazed and confused enough with the whole thing they don't even care to say anything when I'm lighting torches and wielding knives in the back of the house, they just figure I must know what I'm doing (which I do), and that's good enough for them.
Gabe15
07-09-2008, 12:22 AM
I just recently found this video, it has to do with what I was talking about earlier (in another thread perhaps) about the real advantages of TC and why its an important science. This is where bananas really matter, and what drives me to learn and study them more and more.
25OIKGzZZIY
Bananaman88
07-09-2008, 06:21 AM
Gabe,
Just wanted to say thanks to you for taking the time to share all of this with us. I don't plan on trying it at home myself, but being a horticulturist, I've always been interested in the whole TC process. Great job!
damaclese
07-09-2008, 07:44 AM
Gabe,
thank you so much for all the fine information regarding TC. It almost makes me want to try. if i only had the space and time maybe after i get the green house built all start a lab its allot less water intensive then growing out pups at least in the beginning. have you giving any thought to are conversation regarding the contamination sources? perhaps there are people that could be contacted regarding this subject whom mite have come across this in there own Tc labs, or whom mite have discussed this with other Technions? on a slightly different note. I was thinking about how one could Tc Ae Ae. As has been discussed in other threads that it cant be done do to the fact that the variegation is not constant throw the entire meristem. I was thinking about that. As long as you got some of the two different tissues in the sample wouldn't it come true? i would think this would involve knowing exactly were the line of deviation is. and always making sure that when you divide that you get both types of tissues in the Varese samples. dos that make any sense? so in assents one would have to develop a technique for showing the two different tissues in the sample. has any one tried this? Perhaps a dye of some sort that sticks to the two in different intestacy delineating the different types of cells. Or using a microscope to see the varying lines of demarcation when cutting the sample up. Just some thoughts im sure this has been tryed but mabe not you never know!
Gabe15
07-09-2008, 10:02 AM
have you giving any thought to are conversation regarding the contamination sources? perhaps there are people that could be contacted regarding this subject whom mite have come across this in there own Tc labs, or whom mite have discussed this with other Technions?
I emailed someone who has tried it before, and also failed, but am waiting to hear back on their experiences. I also know a few other experts in the field, and will see what they think about it.
I was thinking about how one could Tc Ae Ae.
I have pictures of TC Aeae and have talked in person with the lady who did them. She claimed she didn't do anything special and treated them like all the other Musa. My boss thinks she knows how to do it to keep the variegation, and we would love to experiment with it, but don't have a source for any plants on the island right now. Its actually easier to get them on the mainland than in Hawaii, and banana pups cannot be taken between islands, so they would need to be growing on Oahu already. Perhaps if I can get my hands on a few one day we will try.
damaclese
07-09-2008, 10:10 AM
I emailed someone who has tried it before, and also failed, but am waiting to hear back on their experiences. I also know a few other experts in the field, and will see what they think about it.
I have pictures of TC Aeae and have talked in person with the lady who did them. She claimed she didn't do anything special and treated them like all the other Musa. My boss thinks she knows how to do it to keep the variegation, and we would love to experiment with it, but don't have a source for any plants on the island right now. Its actually easier to get them on the mainland than in Hawaii, and banana pups cannot be taken between islands, so they would need to be growing on Oahu already. Perhaps if I can get my hands on a few one day we will try.
thanks for the speedy reply well the Ae Ae would by far be one of the most profitable Tc projects do to the high cost of the naturally grown plants id like to see thees rare plants proliferate so the price could moderate letting more people have the joy of growing these vary beautiful musa
Dean W.
07-09-2008, 10:19 AM
Another great video, Gabe! I find it very interesting. I wish you well w/ your studies. The banana world can use more experts. :banana-computer:
jpfloors
07-09-2008, 11:52 PM
I have pictures of TC Aeae and have talked in person with the lady who did them. She claimed she didn't do anything special and treated them like all the other Musa. My boss thinks she knows how to do it to keep the variegation, and we would love to experiment with it, but don't have a source for any plants on the island right now. Its actually easier to get them on the mainland than in Hawaii, and banana pups cannot be taken between islands, so they would need to be growing on Oahu already. Perhaps if I can get my hands on a few one day we will try.
Maybe you can get together with someone here in the domestic states and teach them your boss's hypothetical method! I'll volunteer!!!! Too bad I don't have an AeAe :drum: YET! :goteam:
Tropicallvr
07-10-2008, 11:41 AM
Thanks for all the info Gabe. I'm wondering- can you take a diseased plant and tissue culture it, and end up with disease free plantlets?
nucci60
07-10-2008, 12:38 PM
thanks for the speedy reply well the Ae Ae would by far be one of the most profitable Tc projects do to the high cost of the naturally grown plants id like to see thees rare plants proliferate so the price could moderate letting more people have the joy of growing these vary beautiful musa
Wow,that would be great! Look what it did for Siam Ruby. I see them as low as $9.95 on Ebay now.:waving:
xavierdlc61887
07-10-2008, 03:47 PM
WOW that video is awesome ;D
Gabe15
07-10-2008, 07:11 PM
Thanks for all the info Gabe. I'm wondering- can you take a diseased plant and tissue culture it, and end up with disease free plantlets?
I would imagine it depends what disease it is, especially what kind (virus, fungus, insect etc...). I have read somewhere that bunchy top (a virus) does not transfer in culture, it may just be with some varieties under special circumstances though, Im not sure. I also know that other viruses definitely carry over, but I dont know any specifics.
microfarmer
07-11-2008, 12:16 AM
:lurk:
sandy0225
01-21-2009, 10:55 AM
plant patent #15,255, Little prince musa...you should get rid of those or pay the royalties. Watch what you culture unless you get appropriate licenses. Sorry to rain on your parade...
harveyc
01-21-2009, 11:46 AM
I believe he already killed them anyways....
On a related matter: Do we also need to pay royalties if we plant pups from a purchased plant?
Gabe15
01-21-2009, 12:46 PM
As I understood it, its only patent infringement if you plan on selling patented plants. I don't know how they would catch you propagating your own plants for yourself. If they are really that concerned about it then they can come after me, but its not going to stop me from creating mats of miniature bananas to plant around the dorms! I don't plan on selling any 'Little Prince' plants, they are easy to find elsewhere and I have things in TC that I think people will be much more interested in that are under no restrictions.
As Harvey mentioned, all of my TC experiments from last summer died anyway (issues with shipping...I gave my mom directions but sometimes you just gotta do things yourself! haha). However, after a successful collecting mission at the Denver Botanic Gardens over winter break I got a happy number of them back and they are back in TC. If anyone has things they would like for me to try, I'm better prepared to do that now than I was over the summer since I'm back at my lab again.
Gabe15
01-21-2009, 01:05 PM
Over the summer I had prepared tissue cultures of the following and was able to get back the ones listed as "recovered".
Some cool little red thing from China- recovered
Little Prince- recovered
"Red Raja" (my own temporary name for an unknown)- recovered
M. balbisiana x M. dasycarpa #1- recovered
Musella lasiocarpa- recovered
Siam Ruby- lost, but can get it here now so not a problem
Goldfinger- lost, but can get it here
Truly Tiny- lost
M. cheesmanii- lost
California Gold- lost
Ensete perrieri- lost
Ensete ventricosum "Mozambique form"- lost, might be able to get back someday
Hua Moa Freakazoid sport- lost...probably forever
probably interesting unknown seedling- lost...never to return
harveyc
01-21-2009, 01:27 PM
Why is it that when we buy stone fruit trees, roses, etc. they all have a plant patent label on them. However, I've seen Little Prince without such a tag?
Sandy, when you buy a flat of TC plants of something like Little Prince, do they each come with a tag for you to include when you sell them? Also, I'm not familiar with the patenting of something that self propagates such as bananas. How does the patent laws apply to such things?
sandy0225
01-21-2009, 04:42 PM
When you buy a patented plant to resell, you pay a royalty on the plant that goes back to the developer of the plant. That's why people want to patent them...
They should come with a tag that states it's a patented plant. If you want to sell those plants, you have to pay the royalty. You're not authorized to reproduce the plant and sell it. I guess for yourself it would be ok, I'm not really clear on that.
I just know I can't do it and then sell them or pass them on as named varieties. Big no no! Since Gabe wants to go further into this, possibly for $$, I thought he might want to know to confine himself to non-patented varieties.
I have a friend who purchased propagation licenses on several plants--it was expensive. Then he has to collect the royalties on the plants he produces and sells and forward it back to the owner. Crazy huh? And he's supposed to keep tabs on how many he produces vs how many sells in case they want to audit him.
Gabe15
01-21-2009, 10:36 PM
Since Gabe wants to go further into this, possibly for $$, I thought he might want to know to confine himself to non-patented varieties.
Thank you for the info, but as stated, I am not selling any of the patented varieties, or for that matter, any varieties at this point. Really all I want is to do is have a Little Prince desktop banana in my dorm, and to try a few outside in my garden. I put as much as I can into TC backup because it is very likely the plants outside will contract Bunchy Top Virus at some point. With the backups from the lab I can generate more, but if I only have one small plant and I lose it, then I will no longer have that variety.
sandy0225
02-04-2009, 03:33 PM
I hate to seem like I'm not letting this go, but it's not just if you're going to sell them. It's if you're propagating them at ALL. It's just a no-no, no matter how tempting it is. Even if you have "warm fuzzy feelings for the plant and just don't want to lose it". If you lose it, you buy another. Sorry, I didn't make up the rules, I just play within them.
From the website where you register plant patents:
Patents Guidance, Tools & Manuals (http://www.uspto.gov/web/offices/pac/plant/#5)
Rights Conveyed by a Plant Patent
Grant of a patent for a plant precludes others from asexually reproducing or selling or using the patented plant. A plant patent is regarded as limited to one plant, or genome. A sport or mutant of a patented plant would not be considered to be of the same genotype, would not be covered by the plant patent to the parent plant, and would, itself, be separately patentable, subject to meeting the requirements of patentability. A plant patent expires 20 years from the filing date of the patent application. As with utility applications, when the plant patent expires, the subject matter of the patent becomes public domain.
harveyc
02-04-2009, 05:23 PM
Sandy, I wish you would drop this or start a new thread and this is taking on the character of "flaming". First you assumed Gabe was "going further with this" and now you're criticizing something which in just your last post you had guessed "would be okay".
When you buy a patented plant to resell, you pay a royalty on the plant that goes back to the developer of the plant. That's why people want to patent them...
They should come with a tag that states it's a patented plant. If you want to sell those plants, you have to pay the royalty. You're not authorized to reproduce the plant and sell it. I guess for yourself it would be ok, I'm not really clear on that.
I just know I can't do it and then sell them or pass them on as named varieties. Big no no! Since Gabe wants to go further into this, possibly for $$, I thought he might want to know to confine himself to non-patented varieties.
I have a friend who purchased propagation licenses on several plants--it was expensive. Then he has to collect the royalties on the plants he produces and sells and forward it back to the owner. Crazy huh? And he's supposed to keep tabs on how many he produces vs how many sells in case they want to audit him.
Now, I'm sure you are aware, that some of your own online customers should be paying "use" taxes for their online purchases from you (though plants grown for food are not subject to California residents they are in some other states). Do you take the same steps to enforce these laws and reminding your customers about their obligations?
Sorry...end of rant.
Moving on,
Harvey
sandy0225
02-04-2009, 05:49 PM
Sorry, I wasn't trying to flame anybody. I just thought this stuff was interesting. I didn't know the answers so I researched it more, and updated with what I found out. I thought people might want to know. But I guess we really don't...
Sorry to offend anyone....
jigar1231
04-19-2009, 03:52 AM
Here is a short video of the actual tissue culture initiation. It starts out with a plant that has been whittled down to a piece of tissue with some pseuodstem above the meristem and some corm below the meristem. The two clips are of two different plants, but nothing is missed in the process. This is part of a video I'm working on detailing the full process.
p4DUwSGJYNE
hi,
if you have a full video or procedure for banana plant tissue culture
then please send me on jigar1231@yahoo.com
Jack Daw
04-20-2009, 08:32 AM
Considering that you are experts with quite many experience, I would like to ask you some details.
I have been wondering for quite some time:
- What is the precise list of tools, liquids and materials needed for succesful tissue culturing process?
- How much would it cost to make a smaller lab for not large amounts of plants?
- Did you use someone's advice/help on fitting your lab with tools...?
As I'm growing more and more interested about this topic, I would be really glad, if you could sum up your experience and knowledge into a list of all the necessary things, materials and stuff and their apx. costs
What are the special requirements for the room (future labs), meaning like sun/no sun, wet/dry, cold/medium/hot and so on... just everything that crosses your mind. That estimate would be lovely, just to see how much it would be.
If there's been a post like this already, I apologize, but I didn't find it. Just link me. :D Thanks
Dalmatiansoap
04-20-2009, 09:06 AM
That the spirit Jack. Do some TC for Slavic members:)
:woohoonaner:
Jack Daw
04-20-2009, 09:12 AM
That the spirit Jack. Do some TC for Slavic members:)
:woohoonaner:
That wasn't the point, I'm just curious in knowing, how much would it cost. I'm not about to start a lab, at least not until I fully understand what goes with it, what are the monthly/annual costs, what can I expect of building problems etc.
I also take in consideration going to our university of Medicine or Natural Sciences and ask them, if it would be possible to use the laboratory, considering some "rent" and of course, bringing my own material. It all depends on how much the total costs are and how much material and technology is needed. :D And the point was "European", not just "Slavic" ... :) There are lots of eager and pashionate collectors in the EU, the User Map shows that as well. :D
Dalmatiansoap
04-20-2009, 09:18 AM
I was just joking Jack and have to admit that I would also like that we in Europe have a few naner TC experts.
:woohoonaner:
Chironex
04-20-2009, 09:44 AM
Jack, all of this information can be found at: Home Tissue Culture* Group (http://www.hometissueculture.org/)
Jack Daw
04-20-2009, 09:50 AM
Anyone? I know guys you TC a lot... Chironex? Gabe? :nanadrink:
Chironex
04-20-2009, 09:54 AM
The lab can be set-up for under $50. It is not expensive for a home user to do. Once you purchase the chemicals and culture vessels, then all you need are somewhere to keep them and some lights.
As they start to grow and be sub-cultured, that's when the costs increase. You have to buy more media, more containers and it takes more space, so you need more lights. It is best to keep it small production to prevent the cost escalation.
Jack Daw
04-20-2009, 10:47 AM
The lab can be set-up for under $50. It is not expensive for a home user to do. Once you purchase the chemicals and culture vessels, then all you need are somewhere to keep them and some lights.
As they start to grow and be sub-cultured, that's when the costs increase. You have to buy more media, more containers and it takes more space, so you need more lights. It is best to keep it small production to prevent the cost escalation.
Thank you very much Scot, I'll do some more studying. If I have questions, is it OK just to pm you?
Chironex
04-20-2009, 08:15 PM
You can PM me, but you may have to wait for a reply. I have been getting lots of PM's and I have become very busy at work, so I don't always get to the PM's right away.
pinkyteng87
12-18-2009, 09:07 AM
hi Gabe,
Do u mind to tell ime wats de media dat u used to culture banana at home?
novisyatria
02-16-2010, 05:37 PM
Hi Gabe
Glad to hear you work in Tc also like me. can you post your tc plant? and what type you tc now?
sddarkman619
08-03-2012, 12:14 AM
Over the summer I had prepared tissue cultures of the following and was able to get back the ones listed as "recovered".
Some cool little red thing from China- recovered
Little Prince- recovered
"Red Raja" (my own temporary name for an unknown)- recovered
M. balbisiana x M. dasycarpa #1- recovered
Musella lasiocarpa- recovered
Siam Ruby- lost, but can get it here now so not a problem
Goldfinger- lost, but can get it here
Truly Tiny- lost
M. cheesmanii- lost
California Gold- lost
Ensete perrieri- lost
Ensete ventricosum "Mozambique form"- lost, might be able to get back someday
Hua Moa Freakazoid sport- lost...probably forever
probably interesting unknown seedling- lost...never to return
Did you ever reculture a california gold?
and why do you think you lost this one?
Thanks
Larry
Gabe15
08-03-2012, 12:44 AM
That was quite awhile ago, I don't remember exactly, but I think I lost the cultures because I was transporting them in the TC bags and some of them broke. I never tried that one again.
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