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Arun kedia
12-31-2016, 08:18 AM
We are starting a tissue culture lab in Nepal. I am searching if cell culture rout is possible. It has to be a well established protocol. Also trying from where to source the technology. Any leads given will be a great help thanks

JBijl
12-31-2016, 09:24 AM
Welcome here Arun and a happy new year

HMelendez
12-31-2016, 09:56 AM
Welcome to the banana gang!....:bananarow:

edwmax
12-31-2016, 01:53 PM
We are starting a tissue culture lab in Nepal. I am searching if cell culture rout is possible. It has to be a well established protocol. Also trying from where to source the technology. Any leads given will be a great help thanks


Yes, it is ... but tissue culture at the (single or very few) cellular level requires highly specialize labs and equipment.

I think it might be well for you to define which "cell culture rout" or method to which you refer. There are several methods for "micro-propagation" of banana plantlets. Some of these methods are in-vitro by small labs and similar methods by propagation beds by the farmer.

There is a lot of information and training material available. Do a web search for 'micro propagation of bananas' and 'tissue culture of bananas' There is much info & videos on YouTube too.

2 Links that I have at the moment:
Banana Expert System (http://agritech.tnau.ac.in/expert_system/banana/plantingmaterial.html)

Plant tissue culture techniques of Banana (http://www.slideshare.net/sathes32/tissue-culture-techniques-of-banana)

Richard
12-31-2016, 03:33 PM
We are starting a tissue culture lab in Nepal. I am searching if cell culture rout is possible. It has to be a well established protocol. Also trying from where to source the technology. Any leads given will be a great help thanks

Arun, there is a sub-forum here dedicated to tissue culture:
Tissue Culturing & Other Propagation Techniques of Banana Plants (http://www.bananas.org/f260/)

... Do a web search ...

Most people who are serious about tissue culture -- either for business or hobby -- take 2 college horticultural courses in the subject. Here in the U.S. they are offered by many community (2-year) colleges. Arun is probably aware that there is at least one agricultural college right across the border in India.

sputinc7
12-31-2016, 08:16 PM
The best advice I can give you is to be certain of the species you are cloning and keep good records. There is nothing more frustrating than spending months on a plant to find out it is not what you paid for.

Juicy Bananas
01-01-2017, 01:19 AM
Tissue culture can be done with bare minimum equipment. All the expensive equipment does make it much easier though.

I know of this nice little Asian man who lives near by... he has orchids and other various goodies. We are in process of trying to TC various native nanners.

AdotKarl
02-07-2017, 01:14 PM
My university apparently holds the largest banana gene bank in the world, so you
might have a look here in your search;

International Musa Germplasm Transit Centre (http://www.bioversityinternational.org/banana-genebank/)