View Full Version : how to sterilized banana tissue culture
noliboy33
12-28-2014, 07:59 AM
hi there .. i am new here and i want a discussion regarding sterilization and protocols of banana tissue cukture.. i hope you guys will share some iddeas...
kubali
12-28-2014, 08:53 AM
Welcome aboard,
pull up a chair and have some fun learning.
2woodensticks
12-28-2014, 12:46 PM
to sterilize the medium I use a pressure cooker for my orchid media when I flask them..i have had success in oven,but easer to use pressure cooker...tissue itself..have here 10% bleach 3% hydrogen peroxide mix will surface sterilize..i use a diy glovebox I made from plywood and plastic sheet..i do also use a homebrew agar media of my own recipe...potato,suger,young coconut water,pineapple juice,charcoal,dash of fertilizer,agar powder..
noliboy33
12-29-2014, 08:43 AM
is there a banana enthusiast here knew on how to sterilize to eliminate contamination on tissue culture
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