View Full Version : How to care for tissue cultures or young plants.
PR-Giants
03-22-2014, 02:20 PM
:woohoonaner:
pniksch
03-22-2014, 06:29 PM
Who is the grower, and have you purchased from them before?
Glad to see they're hanging in there and thriving!
Thanks,
Paul
bananimal
03-22-2014, 07:56 PM
Supplier is USDA ars - grin I believe. The PO made them what the are later on arrival. I can't get them - I'm not a commercial grower.
Abnshrek
03-22-2014, 08:18 PM
Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)
PR-Giants
03-22-2014, 10:39 PM
Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)
In the Tropics germplasm are actually very strong, all of the plantlets survived and a few produced sister shoots. Being prepared and having confidence in your ability sure helps. The people who have problems are generally adding too much crap too early, like fish emulsion, azomite, mycorrhizae, and fertilizer. If spending more money will help the plant grow then simply buy a jewel encrusted Tiffany spray bottle.
I chose to set the sleeves so that the germplasm received full morning sun from the very first day and waited about 10 to 12 days before removing the sleeves completely. By hardening the plants more quickly they can grow faster by using the full sun.
This photo was posted on Feb-15, it is of a fully hardened Tigua :ha: height 0.18".
Super tall fully hardened TC
http://www.bananas.org/gallery/watermark.php?file=51221 (http://www.bananas.org/gallery/showphoto.php?photo=51221)
Abnshrek
03-22-2014, 10:43 PM
That is a benefit of being in Banana Heaven.. :^)
Germplasm are much weaker than anything most people will order from WellSprings or the like.. Best thing is to keep them in semi-shade for 2 weeks and don't get them full sun till they are 18" or so for most of the south as it warms up or they will get fried especially come summer. :^)
It generally takes 4 to 5 weeks for plantlets that are removed from culture vessels to reach this size, after the initial hardening process (acclimatization) they will be transferred to a greenhouse.
http://i911.photobucket.com/albums/ac317/1tvg/btsy5ywby_zpse34efb32.jpg
They will need a secondary hardening process for two months in a greenhouse before they can be planted in the field.
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Abnshrek
03-22-2014, 11:03 PM
It generally takes 4 to 5 weeks for plantlets that are removed from culture vessels to reach this size, after the initial hardening process (acclimatization) they will be transferred to a greenhouse.
http://i911.photobucket.com/albums/ac317/1tvg/btsy5ywby_zpse34efb32.jpg
They will need a secondary hardening process for two months in a greenhouse before they can be planted in the field.
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That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)
That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)
Do they give you instructions on how to take care of them? :waving:
That's about the size I've gotten from Well Springs garden, maybe slightly bigger but not much.. :^)
Do they give you instructions on how to take care of them? :waving:
If you follow the instructions from the tissue culture lab, U. S. Department of Agriculture Tropical Agriculture Research Station, your plants are acclimatized in about 2 weeks. I probably saved the instruction sheet, but it matched perfectly to what I observed.
I meant instructions from wellspring gardens do they send you instructions with the plants.
:2738: I've read technical papers on the subject saying it would take three weeks or more.
Your technique of two weeks for hardening is impressive.:08:
The most noticeable difference with your photo is that the plant is tall with small leaves,
and mine are short with large leaves, it would be nice to know the cultivar.
They don't say which cultivar
Preparing Tissue-Cultured Banana Plantlets for Field Planting - pdf (http://www.ctahr.hawaii.edu/oc/freepubs/pdf/BIO-8.pdf)
http://www.cialca.org/files/files/extension_materials/tissue-culture_english.pdf (http://www.cialca.org/files/files/extension_materials/tissue-culture_english.pdf)
Abnshrek
03-23-2014, 11:41 AM
Dan, u don't need no GRIN, u's got friends.
Shrek is sending u a Datil and I'll send u a Patupi the following year,
this way u can harvest both at the same time.
That's right.. Mine should be big enough to flourish this year.. :^)
@ Keith We know how you ( a professional banana grower grows), but you have to factor in people that have no experience growing banana's, and will just buy dirt thinking its all the same, which isn't so.. water crystals or water retainers in soil is one way to kill any banana quick up here, and certainly anywhere North of where I am.
Nicolas Naranja
03-27-2014, 08:36 PM
I put the plants from GRIN underneath a bench that gets misted for about 2 weeks and then I place them on top of the bench.
For agristarts bananas, I put them in partial shade in a wind-protected location for 2 weeks and then move them out.
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