View Full Version : Composition of the TC media
YAKUZA
09-04-2011, 07:39 AM
hi tc scientists.:ha:
what media you use for musa?
Composition of the media.
Greetings Yakuza
jmoore
09-04-2011, 08:24 AM
Try this:
To make 1 litre of media, use distilled water.
2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM
You can add vitamins and coconut water and Indole Acetic acid, but start with this.
YAKUZA
09-04-2011, 08:37 AM
Try this:
To make 1 litre of media, use distilled water.
2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM
You can add vitamins and coconut water and Indole Acetic acid, but start with this.
interesting. you do not use acid?
For example, NAA or IAA
jmoore
09-04-2011, 10:28 AM
NAA is for rooting and IAA can be added, but apparently it's not heat stable so will break down when you autoclave. I read that on another post here, I don't think it's essential and only really used for making callus, which you wont be doing.
YAKUZA
09-04-2011, 12:54 PM
are there members who use a different composition?
kentiopsis
09-04-2011, 05:46 PM
I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this.
Aloha from Honolulu,
Ken
Default Re: Composition of the media.
Try this:
To make 1 litre of media, use distilled water.
2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM
You can add vitamins and coconut water and Indole Acetic acid, but start with this.
YAKUZA
09-05-2011, 12:14 PM
I've been a lurker and am a newbie here. I don't know what the purpose of this medium is or what all the abbreviations refer to. This is a bit esoteric for me. Is it a liquid medium for propagation? I'd be grateful if somebody would help me with this.
Aloha from Honolulu,
Ken
Default Re: Composition of the media.
Try this:
To make 1 litre of media, use distilled water.
2.1 grams MS medium
30 grams sugar
5 mg of BAP (5ml of stock 1000 mg / L solution)
8 grams of agar
1 ml PPM
You can add vitamins and coconut water and Indole Acetic acid, but start with this.
It is a medium for tissue culture
In a nutshell.
vitamins, growth hormones / regulators, sugar, anti mold
the agar makes everything from liquid to solid.
such as gelatin
kentiopsis
09-05-2011, 12:37 PM
Thank you, Yakuza. I'm familiar with tissue culture; it's widely used for orchids and seems like a stone natural for bananas. I plan to retire soon to Thailand and plan to devote serious effort to interesting banana cultivars. I hope I can connect with some Bananas.org members over there.
Aloha,
Ken
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