jarceleste
09-10-2010, 09:41 AM
Hello ppl.:bananas_b
Three weeks ago, I've started my Musella lasiocarpa tissue culture.
In the first 4 days it starts to increase the central part, but after that and till the momment, I don't see any changes in the explant.
I don't have contamination in the media.
I've used MS media with vitamines, and no plant hormones.
Can anybody tell me what's wrong?
I have some photos since the beginning <br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/18/IMG00128-20100823-2242-compiled.jpg?ts=1284128064685<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/28/IMG00129-20100823-2242-compiled.jpg?ts=1284128295427<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/53/IMG00133-20100826-1951-compiled.jpg?ts=1284128340116<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/148/IMG00137-20100826-1953-compiled.jpg?ts=1284128740671<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/68/IMG00141-20100827-2147-compiled.jpg?ts=1284128416285<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/73/IMG00150-20100831-1732-compiled.jpg?ts=1284128456034<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/83/IMG00151-20100831-1732-compiled.jpg?ts=1284128495455<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/103/IMG00152-20100831-1733-compiled.jpg?ts=1284128536298
Three weeks ago, I've started my Musella lasiocarpa tissue culture.
In the first 4 days it starts to increase the central part, but after that and till the momment, I don't see any changes in the explant.
I don't have contamination in the media.
I've used MS media with vitamines, and no plant hormones.
Can anybody tell me what's wrong?
I have some photos since the beginning <br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/18/IMG00128-20100823-2242-compiled.jpg?ts=1284128064685<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/28/IMG00129-20100823-2242-compiled.jpg?ts=1284128295427<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/53/IMG00133-20100826-1951-compiled.jpg?ts=1284128340116<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/148/IMG00137-20100826-1953-compiled.jpg?ts=1284128740671<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/68/IMG00141-20100827-2147-compiled.jpg?ts=1284128416285<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/73/IMG00150-20100831-1732-compiled.jpg?ts=1284128456034<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/83/IMG00151-20100831-1732-compiled.jpg?ts=1284128495455<br>http://www.pikipimp.com/pp/pimped_photo/s/image/54/434/103/IMG00152-20100831-1733-compiled.jpg?ts=1284128536298