View Full Version : Tissue Culture late planting
Narnia
09-02-2010, 07:52 AM
Ther 30 T.C's arrived today but [due to a faxed registration form failing to arrive ]they are about 3 weeks late!
Looking akin to overgrown seedings, with several green leaves and rather dense, long roots. My ? is - because the TC is well advanced from the long stay in the flasks at the Lab, should I put them outside in the sun shaded by double layer of shade cloth, sooner than you normally would . The date on the flasks from the Lab is JULY 25th. THANKS for your wonderful help God Bless Today is Sept 2 ( day 2 of Spring over here)
Jack Daw
09-02-2010, 08:34 AM
I wouldn't risk it, unless you an guarantee warmth at night (provided that u r from the unnamed country down-below). Also, no less than partial shade protection should be used. If and when they are tissue cultures, they were started at the protected greenhouse to guarantee illness-free enviroment, so no direct sunlight. There's no point in hasting it.
Congrats on the new plants. :02:
Gabe15
09-02-2010, 11:49 AM
Putting them into sun will likely kill them. They need to be kept in a very humid environment for at least 1-2 weeks. They need light of course, but it should not be direct sunlight, something like the light they would receive if sitting in a window that never receives direct sunlight would be ideal, or even just putting them under a normal fluorescent light would be fine. Usually I put them up into small containers (2-4in) and cover them with a sheet of clear plastic or put the pots in a clear plastic bag. This is especially critical if they are just coming out of the vessels as the humidity needs to remain very high until they can adjust to being outside of the vessel.
Narnia
09-03-2010, 03:46 AM
Thanks JACK DAWand GABE.
I planted the TC's in a polystrene Tomato Box, not into the polystrene cups I'd prepared; the reason being, the roots were dense and long and too small for the cup. 4" containers would've been good but there were none on hand. So I opted for planting in 2 tomato boxes. Using good new potting mix. Then each box was put into a big plastic bag. This proceedure seems to have worked well. The plants are looking very happy,there's a good amount of moisture in the soil and condensation on the plastic"roof " the bag has made. The temp at night from tonights forecast is:
Temperature 19.0°C
Dew Point 17.1°C
Feels Like 19.0°C
Relative Humidity 89%
THANKS guys for your expertise . God Bless you
Nicolas Naranja
09-03-2010, 02:04 PM
I have grown out everything from 4" TC plantlets to monstrous 12"-15" TC plantlets. The shade requirement is always the same I had mine under window screen in a wind protected area. I think there is a picture of it in my gallery. I put 1500 plants thought that system, they were under shade for a month before I moved them out.
http://www.bananas.org/gallery/watermark.php?file=30928&size=1 (http://www.bananas.org/gallery/showphoto.php?photo=30928)
Narnia
09-04-2010, 07:36 AM
THANKS Nicolas for the photo of the plants .They're all looking good. Every one of 'em!
My little bunch of 30, I hope will develop as well as yours have-- but because they are planted in the box side by side, not in seperate containers, I hope their roots as they grow won't get entwined in each other.
If that happens(!) what then?
Nicolas Naranja
09-05-2010, 06:34 PM
I would try to get them in 6" pots if you can, because it can be quite difficult to separate them if they get too intertwined. Thankfully, bananas can handle some root pruning without dying so you should just be able to cut them directly apart.
Narnia
09-05-2010, 11:56 PM
Thanks Nicolas. I'll get some 6" pots . And some more potting mix.Will 6" pots be adequate 'till its time to plant them out?
Nicolas Naranja
09-06-2010, 07:31 PM
Yes, a 6" pot will be full of roots by the time it is ready to plant, you could go bigger. I won't say that it is the only way or necessarily the right way, but it worked for me.
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