View Full Version : Can someone post a tc guide?
imclueless17
07-09-2010, 05:30 PM
Hi I really want to get into tissue culturing bananas. So my question is can someone post a complete guide to tissue culturing bananas in particular, Including at least some the following: materials needed, what media (or media's) to use to properly grow bananas, how to do all of the work including cutting down the meristem, and how to keep everything sterile. Big thanks to all u guys on the org!
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Abnshrek
07-09-2010, 05:45 PM
Info:Tissue Culturing - Bananas Wiki (http://www.bananas.org/wiki/Info:Tissue_Culturing) :^) Search the T/C threads as well or contact Novi.. via pm.. :^)
Gabe15
07-10-2010, 12:44 AM
Tissue culture is a rather complex process. It's not difficult if you know what you are doing, but to explain every detail in writing would take a very long time, and still be very confusing. More importantly, tissue culture is not unique to bananas at all, so any guides you find about tissue culturing bananas will be more on the specifics of banana in tissue culture, but it will not explain all of the really basic tissue culture things. I recommend you do some research on tissue culture in general, do not even think about bananas at first, and once you understand what it involves and get an idea of how it works, then focus on what is needed specifically for bananas.
My personal feeling is that unless you need to make over 1000 plants, tissue culture is not the best option for the hobbyist looking to propagate. It's fun and interesting and has many non-propagation uses, but if you are just looking to multiply the plants you have, it can be expensive, time consuming, confusing and might not meet your needs.
I have a great tissue culture alternative which I plan to share with the group soon. I am making a fully illustrated guide on techniques that can allow the hobbyist to produce many (not as much as tissue culture but more than enough for the average hobbyist) cloned banana plants in a much cheaper, quicker and easier fashion than with tissue culture.
chasbear
07-10-2010, 04:09 AM
I have a great tissue culture alternative which I plan to share with the group soon. I am making a fully illustrated guide on techniques that can allow the hobbyist to produce many (not as much as tissue culture but more than enough for the average hobbyist) cloned banana plants in a much cheaper, quicker and easier fashion than with tissue culture.
I for one will be anxiously awaiting your guide, as I have also toyed with the idea of starting my own tissue culture lab for my expansion here. Yes, I could use more than 1000 plants at a time, but this also has its own hassles. However, if its "cheaper, quicker and easier," then I'm in!
jmoore
07-10-2010, 07:22 AM
Wow Gabe I'm greatly interested in that.
oakshadows
07-31-2011, 07:01 PM
Tissue culture is a rather complex process. It's not difficult if you know what you are doing, but to explain every detail in writing would take a very long time, and still be very confusing. More importantly, tissue culture is not unique to bananas at all, so any guides you find about tissue culturing bananas will be more on the specifics of banana in tissue culture, but it will not explain all of the really basic tissue culture things. I recommend you do some research on tissue culture in general, do not even think about bananas at first, and once you understand what it involves and get an idea of how it works, then focus on what is needed specifically for bananas.
My personal feeling is that unless you need to make over 1000 plants, tissue culture is not the best option for the hobbyist looking to propagate. It's fun and interesting and has many non-propagation uses, but if you are just looking to multiply the plants you have, it can be expensive, time consuming, confusing and might not meet your needs.
I have a great tissue culture alternative which I plan to share with the group soon. I am making a fully illustrated guide on techniques that can allow the hobbyist to produce many (not as much as tissue culture but more than enough for the average hobbyist) cloned banana plants in a much cheaper, quicker and easier fashion than with tissue culture.
Very interestng, did it happen yet ? Following most of your posts I think this will be one to surely want to see.
Thanks
Gabe15
07-31-2011, 09:59 PM
I'm still working on it. The problem is I had many photos of the process, but due to some unfortunate hard drive accidents I lost many of them. I will be working on it more in the next few months as I will be using the technique for my masters project.
funboy30189
07-12-2012, 07:35 PM
gabe any more progress?
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