Bananas.org - View Single Post - Almost ready for ordering...just a few doubts

rohsen · 09-17-2009, 04:23 AM

Dear Alberto,

in my experience the best growth stimulators or hormones, are the ones that are heat resistant. IBA, NAA, BAP, Kinetin are heat resistant others such as GA3 (gibberelic acid) or TDZ (thidiazuron) are not autoclavable and have to be added to media after autoclaving using a filter to cold sterilize them. Keep this in mind. Also you can use household caustic soda (little granules) to prepare a 1N Naoh solution (40 g of NaOH dissolved in distilled water and made upto 1 litre gives 1N NaOH solution)
measure 25mg of the hormone and disolve in 1N naoh (as little as possible) then add up with distilled water until 25ml, this will give you 25ml containing 1mg of hormone per 1 ml of water. you can store these in the freezer for a long time. do not use undiluted naoh in your media you will have to seriously adjust ph if you do. ( the product of neutralising NaOH with HCl is NaCl or salt the more you neutralise the salter your media, not good that is!)
i have for you, if you wish, protocols for micropropagating mango cashew and avacado

good luck my friend
any questions? ask!

Dave

ps. if you dissolve hormones in alcohol they may come out of solution when you add them to water a better solvent would be DMSO (dimethylsulfoxide) even better then naoh.

09-17-2009, 04:23 AM	#4 (permalink)
rohsen rohsen's Photo Gallery Location: Amsterdam, Netherlands Find me on the map! Name: Dave Rossen Join Date: Nov 2008 Posts: 32 BananaBucks : 24,333 Feedback: 0 / 0% Said "Thanks" 8 Times Was Thanked 74 Times in 21 Posts Said "Welcome to Bananas" 0 Times	Re: Almost ready for ordering...just a few doubts Dear Alberto, in my experience the best growth stimulators or hormones, are the ones that are heat resistant. IBA, NAA, BAP, Kinetin are heat resistant others such as GA3 (gibberelic acid) or TDZ (thidiazuron) are not autoclavable and have to be added to media after autoclaving using a filter to cold sterilize them. Keep this in mind. Also you can use household caustic soda (little granules) to prepare a 1N Naoh solution (40 g of NaOH dissolved in distilled water and made upto 1 litre gives 1N NaOH solution) measure 25mg of the hormone and disolve in 1N naoh (as little as possible) then add up with distilled water until 25ml, this will give you 25ml containing 1mg of hormone per 1 ml of water. you can store these in the freezer for a long time. do not use undiluted naoh in your media you will have to seriously adjust ph if you do. ( the product of neutralising NaOH with HCl is NaCl or salt the more you neutralise the salter your media, not good that is!) i have for you, if you wish, protocols for micropropagating mango cashew and avacado good luck my friend any questions? ask! Dave ps. if you dissolve hormones in alcohol they may come out of solution when you add them to water a better solvent would be DMSO (dimethylsulfoxide) even better then naoh. __________________ Reality Is What You Can Get Away With!! Robert Anton Wilson Last edited by rohsen : 09-17-2009 at 04:38 AM.