Is this correct, Cabe15?
Quartering or dividing your explant is subculturing. the two main reasons for this are: first the nutrients in the media are consumed; someday, nothing left to eat! Secondly, metabolism generates waste products and buildup or accumulation willbecome toxic.
So I assume that a culture with 500cc of medium could stand alone for more time than the same explant with only 100cc of media.
the problem is HOW to recognize or calculate when this is going to happen!
Originally Posted by Gabe15
They usually need at least 1 month of growth between subcultures (quartering), but if you can't get around to it right away they can hang out for awhile without much consequence.
What I tell people who ask about how to tissue culture bananas is that they need to do their own research about tissue culture in general and make sure they are well familiar with all the basics and what it actually involves and the theory behind it. Once that is all understood I can help to how to tissue culture bananas specifically, but it's very difficult and confusing to explain the process of tissue culture of bananas when people are only interested in getting more banana plants without understanding what is involved in the basic process with any plant in tissue culture. Its sort of like I could give you some pointers on how to drive a specific car model, but if you don't know how to drive any car, then anything I tell you is pretty meaningless until you know the basics of driving cars in general.