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Old 09-08-2009, 09:47 PM   #7 (permalink)
Location: CAracas, Venezuela, South America
Zone: Tropics
Name: Alberto Albert
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Default Re: Tissue Culture MS Medium Help

Hi Frankco. Please excuse me for deleting part ( OK, almost all ) of your reply.
IŽm sure weŽll understand each other better this way.

Originally Posted by Frankco View Post

Hi Miguel,
For multiplication you should use between 3mg/L and 5 m/L of BAP depending on what type of banana you are trying to tissue culture. Your other ingredients look fine. Just wondering how much sugar you are using. 30 grams to 40 grams per liter is what you should add.

Miguel is using MSP03. So for banana MULTIPLICATION, I should add 3-5mg/l
of BAP plus 30-40g of sucrose ( sugar ). This should work for multiplication.

Now, for rooting, can I just use the same medium, without BAP, and add
an auxin like IBA, 2.4-D or alpha-NAA? If so , how much?

Here is a video that shows you what to use for your explant.
At the end this guy takes out a shoot? for subculture?
He puts two pieces of explants into the same vessel.
Can you explain that?

"Place this material on it's side and carefully trim the associated corm
so as to reduce the amount present "without damaging" the meristem."

This is from KGBensonŽs article.
CanŽt I quarter this explant?

Thanks, Alberto.
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