It is a lot easier to take all the pups if the plant is potted, but depends upon the age of the pup and how close they are to the stems. I have seaparated a one-inch high pup and it grew well. Here's my pictorial diagram on how to do it, this is hosted over at bananavilla's website:
http://groups.msn.com/BananaVillaIma...atingapup.msnw