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Gabe15 · 11-09-2006, 01:17 PM

I have done embryo rescue germination with bananas before, which is basiclaly TC but instead of meristem you use embryos. Contamination was my biggest problem and immediatly kills the cultures. Everything needs to be sterile, it is very important because the medium that is used will grow mold overnight if not sterile.

D'Andra,
Its really not a matter of wheather your meristem is sterile, its everything else you use, your tools, your medium, your growing environment. During my trials last year I used a UV lamp and anhydrous ethanol on everything except the embryos and even then, mold took over everytime. I am doing a similar project this year and have already purchased over 100ml of PPM, it seems like it will really help.

11-09-2006, 01:17 PM	#10 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,892 BananaBucks : 13,496,672 Feedback: 5 / 100% Said "Thanks" 2 Times Was Thanked 8,246 Times in 2,201 Posts Said "Welcome to Bananas" 8 Times	Re: Going to buy the Tissue Culture kit I have done embryo rescue germination with bananas before, which is basiclaly TC but instead of meristem you use embryos. Contamination was my biggest problem and immediatly kills the cultures. Everything needs to be sterile, it is very important because the medium that is used will grow mold overnight if not sterile. D'Andra, Its really not a matter of wheather your meristem is sterile, its everything else you use, your tools, your medium, your growing environment. During my trials last year I used a UV lamp and anhydrous ethanol on everything except the embryos and even then, mold took over everytime. I am doing a similar project this year and have already purchased over 100ml of PPM, it seems like it will really help. __________________ Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.