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Old 05-21-2009, 08:23 PM   #12 (permalink)
Frankco
 
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Default Re: what exactly is tissue culture?

Hi ArchAngeL01,

Ah, that's where the NAA comes in. With the your last round of multiplication, let the plants get a little bigger. Let them will grow into tall single shoots, 3 to 4 inches. Divide the shoots keeping the base on them and put them in media with .5mg of NAA.

Different strenghts of auxins and types of auxins, such as NAA or IBA will work differently for different species of banana. Reports of up to 2mg per liter of NAA have been found to promote rooting with some bananas. NAA at .5mg per liter will work fine for Musa Basjoo.

The NAA promotes the little banana plants to develop roots. When the bananas get to be about 6 to 7 inches tall, (you'll need tall jars), and have nice roots, take them out of the jars, wash all of the agar off under the sink and plant them in a covered container with good potting soil. Keep them well watered and out of direct sun light.

Let them grow some and slowly increase the amount of light they get and slowly allow more air to circulate into the covered container. You can allow more air in the container by increasing the gap of the cover. Do this slowly, they need time to adjust.

When they get to be about 20 inches tall they should be out in the sunlight and getting fresh air. At that point they are ready to brave the world on their own.
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Frankco
Zone 5
N.W. Indiana
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