Quote:
Originally Posted by Chironex
Gabe, that's great news. Was that 1 microliter/liter or 1 micromole/liter. Just being sure. I think a few of the surviving, uncontaminated E. perrieri are showing signs of growth, but not much!
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1 microliter per liter.
In our lab, we use 6 different mediums. Depending on the variety, they will get either 1/2 strength MS (which is standard) or full strength MS (if they are too slow in the 1/2).Within those, we use either 5µl/L of BA for shoot multiplication, 1 µl/L for shoot elongation, and 0µl/L for rooting.
For embryo rescue, I haven't done enough experiments but have done enough to see that each species responds differently in different media. Sometimes the 5µl/L was too much and they multiplied like crazy with no way of regenerating plants easily, and sometimes the 0µl/L did nothing. So I chose the 1µl/L because it is not extreme like the 5µl/L but still has a little bit in there to help induce some growth hopefully.
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