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Old 01-21-2009, 04:57 AM   #60 (permalink)
Chironex
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Default Re: Releasing seed germination inhibitors and breaking thermodormancy

Quote:
Originally Posted by griphuz View Post
No offence Scot, but we do waht to keep thing sterile-ish,....right?
It's not really necessary as these are intact seeds. Actually, they are in more sterile medium than if in regular soil. They are also inside of enclosed petri dishes within the bag, so there really isn't much that can happen. To put your mind at ease, I did not exhale into the bags. I simply opened a fresh can of cola and sealed the open can inside of the plastic bag. (If this were in embryo rescue or tissue culture, then yes, it would be necessary to keep things as sterile as possible.)

I may devise a way to containerize TC jars and keep them in a controlled atmosphere supplemented with CO2 at the proper amounts. But that will have to wait for more funds. I would need to regulate the CO2 to maintain the proper parts per million within the chamber. That sounds like expensive equipment and gauges.
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