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Gabe15 · 10-04-2008, 08:28 PM

Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare.

10-04-2008, 08:28 PM	#5 (permalink)
Gabe15 Moderator Gabe15's Photo Gallery Location: Oahu, Hawaii Find me on the map! Zone: 12 Name: Gabe Join Date: Jul 2005 Posts: 3,892 BananaBucks : 13,339,811 Feedback: 5 / 100% Said "Thanks" 1 Times Was Thanked 8,237 Times in 2,200 Posts Said "Welcome to Bananas" 8 Times	Re: My home TC lab is open, Finally! Looks good. Before you start trying to culture anything, I would recommend going through your normal procedure of making your medium and putting it in the vessel, and with the hood on, leave the vessel open in there for about 10min or so, then seal it again. If after a few days you don't see any contamination, you should be good to go. If you do see contamination, then check over your whole setup and methods to try to find out where it is coming from. Even with a professional setup and experience, contamination can be a problem and it would be good to make sure you are at least capable of having a truly sterile environment before trying to TC anything rare. __________________ Growing bananas in Colorado, Washington, Hawaii since 2004. Commercial banana farmer, 200+ varieties.