Quote:
Originally Posted by Gabe15
We need a lot more information on your technique before any useful advice can be given.
Do you use an autoclave or pressure cooker to sterilize your media? Do you have a functioning laminar flow hood or other sterile environment? Do you flame sterilize your tools before manipulation?
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I use an autoclave to sterilize the media and tools.
I transfer the plants to the media using laminar flow hood .
Within 1 week most plants die due to contamination.