Re: media
There is a broad spectrum fungicide and biocide on the market called 'ppm' which stands for plant preservation mixture, I think. The concentration is 1ml per litre of media. This may help, I'm using it at the moment, bit early to tell how effective it is yet.
Did you sterilise the explant in bleach before putting it into the media?
How good is your autoclave? Are all of your intruments sterilised prior to use?
The media you're using sounds OK, I think.
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