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Old 10-10-2009, 12:33 PM   #1 (permalink)
Java16
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Default Questions about media

Hi everyone,
I am a horticulture major at Penn State and would like to start experimenting with banana tissue culture. I have a few questions. First, when tissue culturing bananas, do you tissue culture the explants in different media for different stages (for example is it necessary to use one media for shoots and then transfer them into another media for roots?) or can you simply keep them in the same media for shoot and root growth? Also, does anyone have an easy to follow recipe for making media? Thanks
-Dave
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