novisyatria

Hi all
I promise you all to take photo subculture in TC bananas step by step. thats why i I'v done today. enjoy the pic.

Step 1. Explant banana 1month old. ready for subculture 1 (Photo 1)
Step 2. Put the explant on sterile petri dish/plate (Photo 2)
Step 3. Cut vertically become 2 explant (Photo 3)
Step 4. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo4)
Step 5. After 1 month next, explant has grow and ready for subculture 2. explant will have few baby bananas. about4-7 baby (photo 5)
Step 6. Put the explat on sterile petridish (Photo 6)
Step 7. Cut the explant become few. every parts could be consist of 2-3 baby bananas (Photo 7)
Step 8. put the explant/planted in the bootle. media same MS + 5 BAP/l (Photo8). Look at the next thread
Next Step like subculture 1 or subculture 2

Attached Images

	IMGP2243 Step 1 Ready for subculture 1.JPG (78.4 KB, 334 views)
	IMGP2245 Step 2.JPG (81.9 KB, 293 views)
	IMGP2246 step 3.JPG (63.8 KB, 308 views)
	IMGP2247 Step 4.JPG (76.4 KB, 278 views)
	IMGP2244 Step 5 Ready for subculture 2.JPG (77.3 KB, 300 views)
	IMGP2249 Step 6.JPG (70.0 KB, 269 views)
	IMGP2250 Step 7.JPG (66.6 KB, 305 views)

__________________
Novi Syatria
Lab of Tissue Culture , Dept of Forestry, Faculty of Agriculture, Univercity of Bengkulu, Indonesia www.kuljar.com email: novi_syatria@yahoo.com